Comprehensive characterization of an iPSC clone

Characterization of iPSCs by immunofluorescence, FACS, embryoid body formation (EB), RT-qPCR, hPSC Scorecard Panel 

This service spans approximately one month and includes: 

• Immunofluorescence for stem cell markers: OCT4, SOX2, SSEA4, and TRA-1-60 
• Flow cytometry for surface markers TRA-1-60, TRA-1-81, and SSEA4 
• Flow cytometry for intracellular markers OCT4, NANOG, and SOX2 
• RNA extraction, reverse transcription, and qPCR for the following 10 stem cell markers: NANOG, REX1, DNMT3B, hTERT, ZFP42, TDGF1, UTF1, LIN28A, GDF3, and FOXD3. 
• Spontaneous embryoid body formation (21 days of differentiation), RNA extraction, reverse transcription, analysis with the ThermoFisher hPSC Scorecard Panel. 
• RNA extraction, reverse transcription, and qPCR for markers verifying the presence of transgenes and the SeV genome. 

The results obtained during characterization will be sent via email in the form of a report.