Different biological tests are offered to evaluate the therapeutic potential of your molecules of interest. 

Endocrinology 

  • Determination of the inhibitory activity of molecules for different enzymes of steroidogenesis: steroid sulfatase, 17β-HSD1, 17β-HSD2, 17β-HSD3, 17β-HSD5, 17β-HSD7, 17β-HSD10 (Donald Poirier) 
  • Determination of estrogenic, anti-estrogenic, androgenic and anti-androgenic activity in vitro (ER+ and AR+ cells) (Donald Poirier) 

Oncology 

  • Cell viability test (normal and cancer cells*) (e.g.: MCF-10A, Lymphocyte, MCF-7*, T-47D*, ZR-75-1*, LNCaP*, PANC-1*, OVCAR-3*, HL-60*, Shionogi*) ( Donald Poirier) 
  • Evaluation of the antiproliferative activity of compounds on several chemoresistant or non-chemoresistant tumor cell lines 
  • Evaluation of the effect of molecules on the progression of the cell cycle (René C.-Gaudreault) 
  • Evaluation of the affinity of antimitotics for the colchicine binding site (René C.-Gaudreault) 
  • Separation and detection of proteins by electrophoresis (western blot) (René C.-Gaudreault) 
  • Evaluation of the antitumor activity and systemic toxicity of organic compounds on human fibrosarcomas (HT-1080) grafted to the chorioallantoic membrane (CAM test) of chicken embryos. This method makes it possible to quickly evaluate important pharmacodynamic parameters such as embryo toxicity, anticancer activity and antiangiogenic activity of a molecule. In addition, the test can validate the effectiveness of a galenic formulation for intravenous administration of a compound. (René C.-Gaudreault)