Quantitative PCR, qPCR, or real-time PCR is a polymerase chain reaction method that allows continuous “real-time” monitoring of the PCR amplification process by detecting the fluorescence emitted by newly formed PCR products at each cycle to measure the initial quantity of DNA.
This technique is useful for gene expression analysis, pathogen detection, and microbiome analysis.
The service is complete: specific primer design, cDNA synthesis, and qPCR.
The real-time gene expression service by PCR (qPCR) (LightCycler 480, Roche) offers precise quantification analyses using SYBRGreen, TaqMan, or multiplexing technologies:
Gene expression analysis
- Full service: assay design, specific primer design, cDNA synthesis (RT), and qPCR
- Experimentation following the MIQE guidelines
- Absolute quantification (standard curves)
- Relative quantification
Microbiome analysis
- Bacterial load (16S rDNA)
- Specific qPCR
Mouse genotyping
Virus titration