Images from confocal microscopy and electron microscopy can be used to reconstruct objects of interest, whether to highlight them in the overall image or to reveal their three-dimensional (3D) structure. In confocal microscopy, 3D reconstruction makes it possible to visualize the spatial distribution of objects of interest and their mutual interactions. In electron microscopy, 3D reconstruction reveals the spatial ultrastructure of subcellular organelles, in particular images obtained with a focused ion beam.