Epifluorescence and Phase Contrast Microscope: Nikon E600

Alias: 

E600 

Primary Applications: 

Acquisition in epifluorescence DAPI, GFP, mCherry, and CY5 and/or transmitted light (phase contrast)

Specifications

Microscope Stand: 

  • Upright microscope Nikon Eclipse 600

Camera: 

  • Photometrics CoolSnap HQ2 1392 (H) x 1040 (V); 6,45µm/pixel 

Fluorescence Illumination Source: 

  • Mercury arc lamp

Objectives and calibrations : 

Objectives Calibration (bin 1×1) 
Plan Fluor 10x 0.25 NA Ph1  0,668 µm/pixel 
Plan Fluor 20x 0.50 NA Ph1  0,333 µm/pixel 
Plan Fluor 40x 0.75 NA Ph2  0,168 µm/pixel 
Plan Fluor 60x 0.85 NA  0,113 µm/pixel 
Plan Fluor 100x 1.30 NA Ph3 huile   0,069 µm/pixel 

Other Nikon objectives available upon request. 

Fluorescence Filters: 

  • DAPI: Chroma 31000v2; Ex : 350/50 nm EM : 460/50 nm DM :400 nm LP 
  • GFP: Chroma 41001; Ex: 480/40 nm EM: 535/50 nm DM: 505 nm LP 
  • CY3: Chroma 41002; EX: 535/50 nm EM: 610/75 nm DM: 565 nm LP 
  • CY5: Chroma 31023; EX: 620/50 nm EM: 690/50 nm DM 655 nm  

Acquisition software : 

  • Metamorph 7.7.7