Alias: 

Bob 

Primary Applications: 

Acquisition in epifluorescence and/or transmitted light (phase contrast). 

Specifications

Microscope Stand: 

  • Inverted microscope Zeiss Axio Observer Z.1 

Objectives and calibrations : 

Objectives Calibration (bin1x1) 
   µm/pixel 
A-Plan 10x 0.25 NA  0,630 
LD A-Plan 20x 0.35 NA  0,310 
LD Plan-NEOFLUAR 40x 0.6 NA  0,160 
Plan-Apochromat 40x Korr 0.95 NA  0,160 
EC-Plan NOEFLUAR 63x oil IRIS 0.7-1.25 NA   0,100 

Other Zeiss objectives available upon request.

Camera: 

  • Zeiss Axiocam MRm CCD 1388 x 1040 pixels @ 6.45 µm 

Fluorescence Illumination Source: 

  • LED: Excelitas X-Cite 120LEDBOOST UV 

Fluorescence Filter Sets: 

  • DAPI :  Zeiss Filter Set 1; Ex: 365/12 Em: 397LP DM: 395 nm  
  • GFP :  Chroma 49002 ET-GFP; Ex: 470/40 nm Em: 525/50 nm DM: 495 nm  
  • mCherry : Zeiss Filter Set 45; Ex: 560/40 nm Em: 630/75 nm DM: 585 nm  
  • CY5 : Chroma 49006 ET-CY5; Ex: ET620/60 nm Em: ET700/75 nm DM: 660 nm LP 

Acquisition Software: 

  • Zeiss Zen Blue 2.0