Derivation of a mouse strain allows for achieving a compliant health status by removing all potentially present pathogens.
Derivation with cryopreservation of sperm and embryos
This procedure allows for achieving a compliant health status while benefiting from the advantages of cryopreservation. For this procedure, you must provide a minimum of 2 males aged between 3 and 6 months. It is possible to perform the procedure with only one male; however, the yield may be affected.
The procedure involves harvesting epididymal sperm and freezing them. To ensure the viability of the sperm, validation will be conducted. This involves thawing a straw of cryopreserved sperm, performing in vitro fertilization, and observing the first cell division upon incubation (two-cell stage). The resulting embryos will be implanted into pseudopregnant females. Once the pups are weaned, a health assessment will be conducted before transferring the animals to the researcher. The costs related to this health assessment are not included in the procedure cost.
The cost of this procedure includes the following:
- Guarantee of 18 straws of cryopreserved sperm
- Minimum guarantee of one breeding pair (1 male and 1 female)
Please note that fertilization will be performed using eggs from “wildtype” females (genetic background corresponding to that of your strain). For example, if the sperm from a homozygous male is cryopreserved, the mice produced after thawing will automatically be heterozygous, and further breeding will be necessary if you wish to obtain homozygous mice.
This procedure allows for decontamination of a strain while benefiting from the advantages of cryopreservation. For this procedure, you must provide either 2 males to obtain fresh sperm along with 20 to 25 females, or 2 straws of frozen sperm and 20 to 25 females.
The procedure involves superovulating females to collect eggs and performing in vitro fertilization with fresh or frozen sperm. The resulting embryos will be cryopreserved, and one straw of embryos will be used for implantation into a pseudopregnant female. Once the pups are weaned, a health assessment will be conducted along with the transfer of animals to the researcher. The costs related to this health assessment are not included in the procedure cost.
The cost of this procedure includes the following:
- Guarantee of 10 straws of cryopreserved embryos
- Minimum guarantee of one breeding pair (1 male and 1 female)