Laser Scanning Confocal Microscope: Olympus FV1000

Alias: 

FV1000 

Primary Applications: 

High-resolution image acquisition ~200 nm (x, y), FRAP protocol by acceptor photobleaching. High-resolution 3D acquisition

Specifications

Microscope stand:

• Inverted microscope Olympus IX81

Confocal: 

• FV1000: 4 PMTs including 2 spectral detectors
• PMT1 and PMT2: spectral detection
• PMT3: RFP emission filters: BA575-620 nm and BA575-675 nm
• PMT4: CY5 emission filter BA655-755 nm

Objectives: 

• UPLANSAPO 20x 0.75 NA 
• UPLANSAPO 40x 0.90 NA 
• PLANAPO N 60x 1.42 NA OIL 
• UPLANSAPO 100x 1.40 NA OIL 

Laser Sources (for acquisitions): 

• Diode Lasers 405 nm, 559 nm, and 635 nm
• Argon-ion Laser Showa Optronics GLG3135: 458 nm, 488 nm, and 515 nm

Fluorescence Observation Light Source: 

• Mercury arc lamp

Other Components: 

• SIM module for simultaneous laser stimulation during acquisition for FRAP or FRET protocols
• Environmental chamber 37^C/5%CO²