Alias: 

LSM 900 

Key Applications: 

High-resolution imaging (~140 nm in x,y) with AiryScan II module. Z-stack acquisition. FRAP (Fluorescence Recovery After Photobleaching). 

Specifications

Microscope Stand: 

  • Inverted microscope Zeiss Axio Observer Z.1 

Confocal Head: 

  • LSM 900 with 2 PMT GaAsP detectors and 1 AiryScan II GaAsP detector

Objectives: 

  • Plan-Apochromat 20x 0.80 NA 
  • Plan-Apochromat 63x OIL 1.40 NA 
  • C-Apochromat 40x 1.1 NA Water 
  • Plan-NeoFluar 40x OIL 1.30 NA 
  • LD Plan-NeoFluar 40x 0.60 NA KORR 

Laser Sources (acquisition): 

  • Laser diodes: 405 nm, 488 nm, 561 nm, 639 nm 

Filters (acquisition): 

  • Variable spectral detection (VSD) 

Fluorescence Illumination Source: 

  • Exfo X-Cite metal halide 

Fluorescence Observation Filters: 

  • DAPI Zeiss filter set 49: EX 365/30 nm — EM 445/50 nm 
  • GFP Zeiss filter set 10: EX 470/40 nm — EM 540/50 nm 
  • RFP Zeiss filter set 20: EX 549/12 nm — EM 608/65 nm 

Software: 

  • Zeiss Zen Blue 3.3 

Other Components: 

  • Environmental chamber at 37°C and 5% CO2 for 35 mm plates and petri dishes 
  • Motorized stage 
  • Transmitted light detector (T-PMT)