{"id":96660,"date":"2026-05-28T15:15:39","date_gmt":"2026-05-28T19:15:39","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/?page_id=96660"},"modified":"2026-06-23T14:39:13","modified_gmt":"2026-06-23T18:39:13","slug":"sequencage-nanopore-nouveau-service","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/sanger-sequencing\/sequencage-nanopore-nouveau-service\/","title":{"rendered":"Nanopore Sequencing &#8211; New Service"},"content":{"rendered":"<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Director<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Vincent Raymond, M.D., Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Managers<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        L\u00e9a Lambert, M.Sc. <br \/>\r\nPatrick Laplante, M.Sc.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">SANGER sequencing platform<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center, CHUL<br \/>\r\n2705, boulevard Laurier, TR-72<br \/>\r\nQu\u00e9bec, Qu\u00e9bec<br \/>\r\nCANADA G1V 4G2\n                                    <\/div>\n                                \n                                                                    <div class=\"mb-1\">\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2299 \">+1 418-654-2299 <\/a>\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-525-4444, ext. 42299 \">+1 418-525-4444, ext. 42299 <\/a>\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2298 (fax)\">+1 418-654-2298 (fax)<\/a>\n                                                                            <\/div>\n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:sequences@crchudequebec.ulaval.ca\" title=\"sequences@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">sequences@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                            \n                                            <div class=\"buttons mt-5 flex flex-col flex-wrap gap-4 max-w-72\">\n                                                            <a href=\"https:\/\/sequences.ulaval.ca\/murin\/servseq.pageaccueil\" class=\"btn\" target=\"_blank\">Connection<\/a>\n                                                    <\/div>\n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                    \n                                            <div class=\"highlight-text md:mb-12 mb-7\">\n                            <p>\n                                The Sanger Sequencing Platform now offers a brand-new Whole Plasmid Sequencing service!\n                            <\/p>\n                        <\/div>\n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p>This new generation of sequencing, developed by Oxford Nanopore Technologies, provides long-read sequencing capabilities that enable the complete sequencing of plasmids without the need for primers. Our expertise is currently focused on plasmids, but the possibilities offered by ONT technology are extensive. Stay tuned!<\/p>\n<div>\n<p>Our current turnaround time is 1\u20132 weeks, depending on the number of samples received. Please contact us by email to schedule the submission of your samples.<\/p>\n<h4>Technique<\/h4>\n<p>The use of a Rapid Barcoding Kit enables the simultaneous sequencing of multiple samples by tagging each DNA molecule with a unique sample-specific barcode. A transposase randomly cleaves the DNA and attaches a \u201ctag\u201d sequence to the plasmid. The library of tagged plasmids is then ligated with an adapter that allows nanopores to sequence all DNA strands and to sort the resulting data based on their associated barcodes.<\/p>\n<p>Oxford Nanopore devices use a flow cell composed of an electro-resistant membrane embedded with nanoscale pores (nanopores). Each nanopore measures the electrical current passing through it via an electrode connected to a channel and a sensor chip. When a DNA molecule passes through the pore, the electrical current is disrupted. This current change generates a characteristic signal known as a \u201csquiggle,\u201d which is subsequently decoded by a basecalling algorithm. Each DNA base produces a distinct squiggle pattern, and the succession of these signals allows for the determination of the plasmid sequence.<\/p>\n<\/div>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n\n\n<section id=\"\" data-block=\"block_41a4b46cea4b2de9d53f2a9c0f4d5efe\" class=\"block-accordions align bg-white\">\n    <div class=\"container\">\n        <div class=\"padd\">\n            <div class=\"wrap\">\n                <h2 class=\"lg:mb-14 mb-7 sm:text-48 text-36\">Procedures<\/h2>\n\n    <div class=\"w-full divide-y divide-blue-200 border-blue-200 last:border-b border-t\">\n                                    <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    DNA Preparation\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>Here are the sample submission and preparation guidelines: <a href=\"#FAQ-Sanger\">click here<\/a><\/p>\n<div>\n<h5>PLASMID<\/h5>\n<ul>\n<li>Concentration: 50 ng\/\u00b5L in 10 mM Tris (pH 8.0) or H\u2082O<br \/>\nIt is important to accurately measure the concentration of your DNA. If the concentration is high and dilution is required, it is recommended to re-quantify the sample after dilution to 50 ng\/\u00b5L in order to report the exact concentration on the submission form.<\/li>\n<li>Ratio: A260\/280: between 1.8 and 2.0 and A260\/230: between 2.0 and 2.2<br \/>\nThese ratios are good indicators of DNA purity. Residual RNA, solvents, salts, or proteins in your sample may negatively affect sequencing quality.<\/li>\n<li>Volume: Minimum of 15 \u00b5L per sample. If the concentration does not reach 50 ng\/\u00b5L, please double the volume per sample.<\/li>\n<li>Warning! The main cause of Nanopore sequencing failure is DNA degradation. Do not vortex your plasmids. To ensure DNA quality, it is recommended to run your samples on an agarose gel prior to submission.<\/li>\n<\/ul>\n<\/div>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Primers\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>No primers are required for this type of sequencing.<\/p>\n<div>\n<div class=\"fai-CopilotMessage__actions rmlvq19 ___7qar2c0 fcthzvy\" role=\"toolbar\" data-tabster=\"{&quot;mover&quot;:{&quot;cyclic&quot;:false,&quot;direction&quot;:2,&quot;memorizeCurrent&quot;:true}}\"><\/div>\n<\/div>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Order creation\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>Order creation is currently handled by email.<\/p>\n<div>\n<p>Please contact us by email (<a href=\"mailto:sequences@crchudequebec.ulaval.ca\">sequences@crchudequebec.ulaval.ca<\/a>) to submit your request for Nanopore sequencing services. We will provide you with the procedure and the necessary documents to complete your service request.<\/p>\n<\/div>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                        <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/section>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n<section id=\"FAQ-Sanger\" data-block=\"block_3807f63c674d12d498bbbb57f335992f\" class=\"block-accordions align bg-white\">\n    <div class=\"container\">\n        <div class=\"padd\">\n            <div class=\"wrap\">\n                <h2 class=\"lg:mb-14 mb-7 sm:text-48 text-36\">FAQ<\/h2>\n\n    <div class=\"w-full divide-y divide-blue-200 border-blue-200 last:border-b border-t\">\n                                    <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    What is included in the results?\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>The analysis is performed using the wf-clone-validation workflow from the Epi2Me software. The results include:<\/p>\n<div>\n<ul>\n<li>An assembly report (wf-clone-validation-report.html)<\/li>\n<li>A consensus sequence (FASTA)<\/li>\n<li>An annotated map generated by pLannotate (.gbk)<\/li>\n<\/ul>\n<\/div>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    What are the fees for this service?\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>Whole plasmid Nanopore sequencing costs $20 per sample.<\/p>\n<div>\n<div class=\"fai-CopilotMessage__actions rmlvq19 ___7qar2c0 fcthzvy\" role=\"toolbar\" data-tabster=\"{&quot;mover&quot;:{&quot;cyclic&quot;:false,&quot;direction&quot;:2,&quot;memorizeCurrent&quot;:true}}\"><\/div>\n<\/div>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    What are the service hours?\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>Nanopore sequencing is offered once every 1 to 2 weeks, depending on the number of samples received. To be included in the Monday sequencing run, samples must be received no later than Thursday afternoon of the previous week. Results are expected to be available by the following Wednesday and include, among other outputs, a plasmid map, the FASTA sequence, and a sequencing report.<\/p>\n<div>\n<p>Please contact us at <a href=\"mailto:sequences@crchudequebec.ulaval.ca\">sequences@crchudequebec.ulaval.ca<\/a> to schedule the submission of your samples.<\/p>\n<\/div>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    What are the DNA submission and preparation guidelines?\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <div>\n<ul>\n<li>Always label your tubes with the sample name, your name, and the mention \u201cNanopore\u201d or \u201cONT\u201d to distinguish them from samples intended for Sanger sequencing.<\/li>\n<li>Purification methods compatible with Nanopore sequencing include magnetic beads or high-quality commercial spin columns.<\/li>\n<li>We strongly recommend performing a second wash during miniprep-style purifications, as well as a dry spin before elution to remove any residual contaminants from the column.<\/li>\n<li>The use of phenol\u2013chloroform negatively affects Nanopore sequencing flow cells by damaging their physical components and inhibiting the sequencing process. Do NOT send samples purified using this method.<\/li>\n<li>All samples must be submitted in 1.5 mL microcentrifuge tubes (Eppendorf-style). When submitting multiple samples (24 or more), plates or tube strips are easier for us to handle. This also reduces labeling effort and minimizes handling errors.<\/li>\n<li>We recommend protecting your samples in a 50 mL plastic tube (a tip box can also be suitable). The use of bubble wrap is also recommended.<\/li>\n<\/ul>\n<\/div>\n<p><img decoding=\"async\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/10\/Image-Sanger-1.jpg\" \/> <img decoding=\"async\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/10\/Image-Sanger-2.jpg\" \/><\/p>\n<ul>\n<li>\n<div>\n<div id=\"copilot-message-r1q3\" class=\"fai-CopilotMessage__content r1izxv8h ___7qar2c0 fcthzvy\" dir=\"auto\">\n<div>\n<div dir=\"auto\">\n<div class=\"___1vve6yh f22iagw f1vx9l62 f122n59 f3bhgqh f1mnxvew fly5x3f\">\n<div class=\"___j4vmuw0 f22iagw f1vx9l62 f1869bpl fly5x3f fbc6vix\">\n<div tabindex=\"-1\" data-testid=\"loading-message\" data-fui-focus-visible=\"\">\n<div>\n<div>\n<div id=\"response-idr1q1\" class=\"___1yop6mf f336tjw f9ijwd5 fz7g6wx fod5ikn f1s184ao\">\n<div class=\"___1j4t0a1 f1lmfglv f1abmfm4\" dir=\"auto\" aria-hidden=\"true\" data-testid=\"markdown-reply\" data-message-type=\"Chat\">\n<div class=\"___1obcdld f22iagw f1vx9l62 f1i1t7jz fk15e71 f1k953kz f137keqj f1rncccw f1stllg3 f1w4kmmc f1tric3v f10dk5ic fx8cdsv f16nlwsl f1eookvz fhjrts3 fqugt2f f18d18k8 f1v1vhmp f1hdmul3 f198vjnp f2dshw1 fjpjndq fnmj2pj ffrrb40 f1mon110 fhajzam fimndk6 fxv4dng fb1rn9k f59wlhv f1xmfh30 f18yf5o5 fmr6chf fw8qy2q ff161sd f1qr7t77 f1yyogn1 foogchg fp5872b f1tpb8gf f1hdgrc8 f1vatr43 frkxccd f9pdzkb f158oiaz ft5kggy f5l4ui7 f13j03li f18xdoka f8off4e f1go6zfc f128n2ra f1jpxw2j f1lwd9n0 fy0b8hm f1g2633v fx4kvgi fkvolse f1gxrt3a f1os76ua f1js9cnw f1g87ej0 freluf8 f1yggaq6 f2i3chp f1151osp florpoh f35kzfz ftp9m57 f1e288vi fz62hnz fixvuys f1nmofwh fc1c6mv f18a2er7 fhvkrdl fhxnn68 f1oce4bo fvhlfan f7tc0t0 f1ngf1n2 ffrzq6x f1yjiq2m f1aqdgql f1huvaf f10ij78l f1k94sa4 fkmfumq f1jvzkyl f1eu24ob f1xbfc1f fmf0lwf f107dsy8 f3w566c f4648sl f13zpkng f7unjfp f8p1rz fzu6h1w f1i1bi6h f1smo7hi f1sk1xod f1kgogdq f1gjktkp ft608jz f19dd9cz fg3xr24 fhd155k f1b8gl2e fg4b9ou flb4lo fdicbfk fgwcyh1 f1jbds0d f1fb9uea f1stuka4 fcrtu6g fwqh0xn f11w66yg f6e0e65 f1vvgu0 f1607507 fgct6un f1ec1nbe f1m9bycv f1ntoah8 f1b694rt faql4r2 fpjuhzh fmavl6h f1d9bsk fmzhlt0 f145l92p fwkyaqm f129obh1 f19la9g f127w39w fr0ux9m fxhidck f1cu4h9j f1wnbo7v f1eueg4z fy9c24f f1h2o91j f1iw08fq\">\n<p>If you are using 96-well plates, avoid cell culture plates (not leak-proof). Adhesive films for PCR plates tend to leak and are not recommended for transport. Caps from the same manufacturer as your plates provide a simple and effective solution to this issue.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/li>\n<\/ul>\n<p><img decoding=\"async\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/10\/Image-Sanger-3.jpg\" \/> <img decoding=\"async\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/10\/Image-Sanger-4.jpg\" \/><\/p>\n<div>\n<ul>\n<li>If you are using parafilm to seal your tubes, use the minimum amount.<\/li>\n<li>Do not use adhesive tape to label your tubes, as it tends to come off at \u221220 \u00baC.<\/li>\n<li>Do not use adhesive tape to bundle your samples together, as it is difficult to separate and may smear the ink on the tubes. Instead, use an envelope, a resealable plastic bag (e.g., Ziploc\u00ae), or any other easy-to-handle container.<\/li>\n<li>It is not necessary to place your DNA on ice or dry ice if it is not contaminated with endonucleases.<\/li>\n<li>It is recommended to protect your samples during shipping by mail or courier: use a padded envelope with bubble wrap or a rigid container such as a 50 mL screw-cap Falcon tube.<\/li>\n<\/ul>\n<p>Warning: please ensure that your samples do not arrive over the weekend or on a public holiday.<\/p>\n<\/div>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    \u00c0 quelle adresse envoyer les \u00e9chantillons?\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>For external submissions:<\/p>\n<ul>\n<li>Patrick Laplante<br \/>\nPlateforme de s\u00e9quen\u00e7age et de g\u00e9notypage (CRCHU de Qu\u00e9bec-CHUL)<br \/>\n2705, boulevard Laurier, T0-72<br \/>\nQu\u00e9bec, Qu\u00e9bec<\/li>\n<\/ul>\n<p>CANADA G1V 4G2<\/p>\n<p>Pour internal submissions:<\/p>\n<div>\n<ul>\n<li>Samples can be dropped off at the reception desk of Block T, room TR-72, or at the entrance of the Infectious Disease Research Centre, room RC-709.<\/li>\n<li>You may also deliver them directly to room R-4756 if you have access.<\/li>\n<li>Samples can be sent via internal mail; however, please allow an additional day for delivery.<\/li>\n<\/ul>\n<\/div>\n<p>&nbsp;<\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    For more information&#8230;\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>For more information about Nanopore sequencing or to schedule the submission of samples, please feel free to contact L\u00e9a Lambert directly (<a href=\"mailto:lea.lambert@crchudequebec.ulaval.ca\">lea.lambert@crchudequebec.ulaval.ca<\/a>).<\/p>\n<div>\n<p>To learn more about the technology used for Nanopore sequencing, the following video provides a clear overview of how it works: <a href=\"https:\/\/www.youtube.com\/watch?v=RcP85JHLmnI\">https:\/\/www.youtube.com\/watch?v=RcP85JHLmnI<\/a><u>.<\/u><\/p>\n<\/div>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                        <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/section>\n\n\n<section id=\"\" data-block=\"block_7dc428b9a20ccab12325bc6098f7dcee\" class=\"block-content-image-cta align bg-white\">\n    <div class=\"container\">\n        <div class=\"padd\">\n            <div class=\"wrap\">\n                <div class=\"flex sm:flex-row flex-col justify-between md:items-center lg:gap-x-[8.3%] gap-x-6 sm:flex-row-reverse\">\n                    \n                    <div class=\"lg:w-5\/12 sm:w-1\/2\">\n                        \n                        \n                                            <\/div>\n                <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Plasmid and PCR product sequencing service (to be purified or already purified).<\/p>\n","protected":false},"author":110,"featured_media":0,"parent":92941,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"template-platforms-services.blade.php","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-96660","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Nanopore Sequencing - New Service - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval<\/title>\n<meta name=\"description\" content=\"La plateforme de s\u00e9quen\u00e7age Sanger offre maintenant un tout nouveau service de s\u00e9quen\u00e7age permettant de s\u00e9quencer les plasmides en entier.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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