{"id":92951,"date":"2024-05-14T14:50:51","date_gmt":"2024-05-14T18:50:51","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/genomics-center\/sanger-sequencing\/identification-de-lignee-cellulaire\/"},"modified":"2025-10-08T15:54:30","modified_gmt":"2025-10-08T19:54:30","slug":"cell-line-identification","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/sanger-sequencing\/cell-line-identification\/","title":{"rendered":"Cell Line Identification"},"content":{"rendered":"<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Director<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Vincent Raymond, M.D., Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Manager<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Patrick Laplante, M.Sc.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">SANGER sequencing platform<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center<br \/>\r\nCHUL<br \/>\r\n2705, boulevard Laurier, TR-72<br \/>\r\nQu\u00e9bec, Qu\u00e9bec<br \/>\r\nCANADA G1V 4G2\n                                    <\/div>\n                                \n                                                                    <div class=\"mb-1\">\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2299\">+1 418-654-2299<\/a>\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-525-4444, ext 42299\">+1 418-525-4444, ext 42299<\/a>\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2298 (FAX)\">+1 418-654-2298 (FAX)<\/a>\n                                                                            <\/div>\n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:sequences@crchudequebec.ulaval.ca\" title=\"sequences@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">sequences@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                            \n                                            <div class=\"buttons mt-5 flex flex-col flex-wrap gap-4 max-w-72\">\n                                                            <a href=\"https:\/\/sequences.ulaval.ca\/murin\/servseq.pageaccueil\" class=\"btn\" target=\"_blank\">Login<\/a>\n                                                    <\/div>\n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                    \n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p>The GlobalFiler\u2122 amplification kit is a multiplex test that identifies a set of 24 markers (STRs), allowing for the identification of human cell lines from genomic DNA. STRs are short repeated DNA sequences. The number of repetitions varies greatly among individuals and alleles. This technique should be used exclusively for research projects.<\/p>\n<p>Here are some examples of its use :<\/p>\n<ul>\n<li>Comparing 2 cell lines\n<ul>\n<li>Cell Line A vs. Cell Line B<\/li>\n<\/ul>\n<\/li>\n<li>Identifying a cell line (for publication)\n<ul>\n<li>Cell Line C vs. databases:\n<ul>\n<li>https:\/\/www.atcc.org\/search-str-database<\/li>\n<li>http:\/\/dsmz.de\/fp\/cgi-bin\/str.html<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<li>Comparing an in-house cell line\n<ul>\n<li>Immortalized Cell Line vs. Original Donor DNA<\/li>\n<\/ul>\n<\/li>\n<li>Verifying the purity of a cell line\n<ul>\n<li>Contamination if more than 2 alleles are present<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><strong>Technique<\/strong><\/p>\n<p>The identification is performed using the Globalfiler PCR amplification kit from genomic DNA.<br \/>\nThe PCR kit includes specific primers for each marker. The resulting PCR will then be migrated by capillary electrophoresis on the DNA Analyzer 3730 XL (Applied Biosystems\u2122). We use the analysis modules described in the Globalfiler user kit.<\/p>\n<p>The kit includes an allelic scale control allowing for the automatic genotyping of most alleles for the kit&#8217;s loci and a positive DNA control for experiment validation.<\/p>\n<p>The raw data is analyzed using GeneMapper.<\/p>\n<p>Two Excel files will be sent to you by email:<\/p>\n<p>The results report:<\/p>\n<ul>\n<li>The results report:\n<ul>\n<li>Consolidates all the results of the 24 markers for each sample as well as the control samples.<\/li>\n<\/ul>\n<\/li>\n<li>Comparison file:\n<ul>\n<li>Allows for easy evaluation of samples\/lines among themselves by obtaining a percentage of homology.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>Our processing and analysis times are approximately 1-2 weeks.<\/p>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\"><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n\n\n<section id=\"\" data-block=\"block_5801dc024b68c65f84bdb696e8176635\" class=\"block-accordions align bg-white\">\n    <div class=\"container\">\n        <div class=\"padd\">\n            <div class=\"wrap\">\n                <h2 class=\"lg:mb-14 mb-7 sm:text-48 text-36\">Procedure<\/h2>\n\n    <div class=\"w-full divide-y divide-blue-200 border-blue-200 last:border-b border-t\">\n                                    <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Sample Preparation\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>We only process pure and high-quality genomic DNA.<\/p>\n<ul>\n<li>Volume : 10-20 uL<\/li>\n<li>Concentration : 25-50 ng\/uL.<\/li>\n<\/ul>\n<p>These samples should be sent in sealed 1.5 mL microtubes (Eppendorf style), strips, or 96-well plates.<\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Shipping Guidelines\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>Please contact us to request our cell line identification. We will provide you with more details on the procedure for sending samples and setting up your project.<\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                        <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Cell Line Identification Service using the GlobalFiler\u2122 PCR Amplification Kit. For comparison with known markers or between 2 cell lines.<\/p>\n","protected":false},"author":10,"featured_media":0,"parent":92941,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-92951","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Cell Line Identification - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/sanger-sequencing\/cell-line-identification\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cell Line Identification - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval\" \/>\n<meta property=\"og:description\" content=\"Cell Line Identification Service using the GlobalFiler\u2122 PCR Amplification Kit. 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