{"id":92949,"date":"2024-05-14T14:51:50","date_gmt":"2024-05-14T18:51:50","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/genomics-center\/sanger-sequencing\/identification-de-bacterie-levure-via-16s-its\/"},"modified":"2025-10-08T15:54:38","modified_gmt":"2025-10-08T19:54:38","slug":"bacteria-and-yeast-identification-via-16s-its","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/sanger-sequencing\/bacteria-and-yeast-identification-via-16s-its\/","title":{"rendered":"Bacteria and Yeast Identification via 16S\/ITS"},"content":{"rendered":"<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Director<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Vincent Raymond, M.D., Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Manager<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Patrick Laplante, M.Sc.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">SANGER sequencing platform<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center<br \/>\r\nCHUL<br \/>\r\n2705, boulevard Laurier, TR-72<br \/>\r\nQu\u00e9bec, Qu\u00e9bec<br \/>\r\nCANADA G1V 4G2\n                                    <\/div>\n                                \n                                                                    <div class=\"mb-1\">\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2299\">+1 418-654-2299<\/a>\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-525-4444, ext 42299\">+1 418-525-4444, ext 42299<\/a>\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2298 (FAX)\">+1 418-654-2298 (FAX)<\/a>\n                                                                            <\/div>\n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:sequences@crchudequebec.ulaval.ca\" title=\"sequences@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">sequences@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                            \n                                            <div class=\"buttons mt-5 flex flex-col flex-wrap gap-4 max-w-72\">\n                                                            <a href=\"https:\/\/sequences.ulaval.ca\/murin\/servseq.pageaccueil\" class=\"btn\" target=\"_blank\">Login<\/a>\n                                                    <\/div>\n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                    \n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p>Microorganism identification techniques are evolving, increasingly favoring PCR-based methods. Indeed, PCR amplification coupled with Sanger sequencing enables the identification of bacteria and yeast. This is achieved by referencing the sequences in the 16S and ITS databases using BlastTM on NCBI. This approach helps identify bacteria or yeast that may produce ambiguous results in traditional galleries.<\/p>\n<p>Please note that for certain genera of bacteria, the sequence may resemble that of two very similar species.<\/p>\n<p><strong>Technique<\/strong><br \/>\nIdentification is carried out by PCR using genomic DNA, colony, or culture broth. The primers are specific to the 16S and ITS regions.<\/p>\n<p><span style=\"text-decoration: underline;\">16S-Bacteria<\/span><br \/>\n16S-27F AGAGTTTGATCCTGGCTCAG<br \/>\n16S-1541R AAGGAGGTGATCCAGCCGCA<br \/>\nAmplicon: About 1500 pb<\/p>\n<p><span style=\"text-decoration: underline;\">ITS-Yeast<\/span><br \/>\nITS1 TCCGTAGGTGAACCTGCGG<br \/>\nITS4 TCCTCCGCTTATTGATATGC<br \/>\nAmplicon: Variable depending on the species, ranging from 600 to 900 bp.<\/p>\n<p>PCRs are purified, quantified, diluted, and sequenced using the Sanger method. The results are provided in the form of chromatograms and DNA sequences. With this sequence, you will be able to perform a BLAST and compare your sequence with those deposited in the database. This will allow you to determine the percentage of homology with certain species of bacteria or yeast, thereby confirming the identification.<\/p>\n<p>Our current processing time is approximately 1 week. Typically, we process samples on Wednesday afternoon, and the results are available on Monday morning.<\/p>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n\n\n<section id=\"\" data-block=\"block_56542389e15c941ff2724d9d789156a1\" class=\"block-accordions align bg-white\">\n    <div class=\"container\">\n        <div class=\"padd\">\n            <div class=\"wrap\">\n                <h2 class=\"lg:mb-14 mb-7 sm:text-48 text-36\">Procedure<\/h2>\n\n    <div class=\"w-full divide-y divide-blue-200 border-blue-200 last:border-b border-t\">\n                                    <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Sample Preparation\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p><a href=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/PDF-Procedure-Envoi-Bacteries-et-levure-Francais.pdf\" target=\"_blank\" rel=\"noopener\"><span data-contrast=\"auto\">PDF Proc\u00e9dure Envoi Bact\u00e9ries et levures (In French)<\/span><\/a><\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Shipping Guidelines\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>Please note that we are not adequately equipped to handle biosafety level 2 microorganisms. Therefore, we only accept samples of biosafety level 1.<\/p>\n<p>Please contact us to submit your request for 16S-ITS identification service. We will provide you with further details on the procedure to complete the service request.<\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                        <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Bacteria\/Yeast Identification Service by Rapid DNA Extraction, PCR Amplification of 16S\/ITS, and Sequencing.<\/p>\n","protected":false},"author":10,"featured_media":0,"parent":92941,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-92949","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Bacteria and Yeast Identification via 16S\/ITS - 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