{"id":92945,"date":"2024-05-13T10:01:32","date_gmt":"2024-05-13T14:01:32","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/genomics-center\/sanger-sequencing\/genotypage-de-marqueurs-microsatellites-microsat-et-autres\/"},"modified":"2025-10-08T15:53:24","modified_gmt":"2025-10-08T19:53:24","slug":"sanger-sequencing-microsatellite-marker-genotyping","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/sanger-sequencing\/sanger-sequencing-microsatellite-marker-genotyping\/","title":{"rendered":"Sanger Sequencing &#8211; Microsatellite marker genotyping (MicroSAT) and others"},"content":{"rendered":"\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Director<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Vincent Raymond, M.D., Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Manager<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Patrick Laplante, M.Sc.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">SANGER sequencing platform<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center<br \/>\r\nCHUL<br \/>\r\n2705, boulevard Laurier, TR-72<br \/>\r\nQu\u00e9bec, Qu\u00e9bec<br \/>\r\nCANADA G1V 4G2\n                                    <\/div>\n                                \n                                                                    <div class=\"mb-1\">\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2299 \">+1 418-654-2299 <\/a>\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-525-4444, ext 42299 \">+1 418-525-4444, ext 42299 <\/a>\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2298 (FAX)\">+1 418-654-2298 (FAX)<\/a>\n                                                                            <\/div>\n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:sequences@crchudequebec.ulaval.ca\" title=\"sequences@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">sequences@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                            \n                                            <div class=\"buttons mt-5 flex flex-col flex-wrap gap-4 max-w-72\">\n                                                            <a href=\"https:\/\/sequences.ulaval.ca\/murin\/servseq.pageaccueil\" class=\"btn\" target=\"_blank\">Login<\/a>\n                                                    <\/div>\n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                    \n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p>Microsatellite markers are short repeated DNA sequences. The number of repetitions varies widely, depending on the species, the individual, and even the allele. Since they are found throughout the genome and are highly polymorphic, they are ideal for genetic mapping.<\/p>\n<p>Genotyping on the DNA Analyzer 3730 XL allows the analysis of DNA fragments labeled with a fluorochrome ranging from 60 to 1200 base pairs. There are several multicomponent matrices available for analyzing different types of fluorochromes.<\/p>\n<p><span style=\"text-decoration: underline;\">Here are those available at the platform:<\/span><\/p>\n<p>DS-33 Matrix Standard Kit (Dye Set G5): 6-FAM\u2122, VIC\u2122, NED\u2122, PET\u2122, and LIZ<\/p>\n<p>DS-36 Matrix Standard (Dye Set J6): 6-FAM\u2122, VIC\u2122, NED\u2122, SID\u2122, TAZ\u2122, and LIZ<\/p>\n<p>Depending on the matrix used, it is possible to automatically analyze 4 to 5 fragments labeled with a different fluorochrome in a single capillary. The same fluorochrome can also be used more than once when the fragments differ by more than 100 base pairs. This allows us to multiplex up to 12 markers per individual in a single sample.<\/p>\n<p><strong>Technique<\/strong><\/p>\n<p>Genotyping of microsatellite markers is done through PCR amplification. These polymorphic regions are amplified by labeling one of the primers with a fluorochrome. Then, through capillary electrophoresis, it is possible to determine the exact length of each microsatellite studied in an individual.<\/p>\n<p><span style=\"text-decoration: underline;\">Applications<\/span><\/p>\n<ul>\n<li>Genotyping of different microsatellites (MicroSAT)<\/li>\n<li>Human cell line identification &#8211; Globalfiler 24 STR Kit<\/li>\n<li>HLA &#8211; Sequencing<\/li>\n<li>Microsatellite instability &#8211; TrueMark MSI Test<\/li>\n<li>Examination of the 3D structure of nucleic acids &#8211; QuShape<\/li>\n<\/ul>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n\n\n<section id=\"\" data-block=\"block_59a01617e26f3b76bd1eb87c48ded200\" class=\"block-accordions align bg-white\">\n    <div class=\"container\">\n        <div class=\"padd\">\n            <div class=\"wrap\">\n                <h2 class=\"lg:mb-14 mb-7 sm:text-48 text-36\">Procedure<\/h2>\n\n    <div class=\"w-full divide-y divide-blue-200 border-blue-200 last:border-b border-t\">\n                                    <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Sample Preparation\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>We only process PCR reactions meeting the following conditions:<\/p>\n<ul>\n<li>Utilizing one or more fluorochromes available on the platform<\/li>\n<li>PCR product length: Between 60 and 1200 base pairs<\/li>\n<li>Clear band(s) on gel, if possible<\/li>\n<li>Volume: Dried PCR pellet or a minimum of 10 \u03bcL of volume<\/li>\n<\/ul>\n<p>These should be sent in a 96-well plate or in 1.5 mL microtubes.<\/p>\n<ul>\n<li>Priority will be given to dried PCR plates.<\/li>\n<\/ul>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Shipping Guidelines\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>Please contact us to submit your request for microsatellite services. We will provide you with more details on the procedure for sending samples, multiplexing, and further information.<\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                        <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Microsatellite Electrophoresis Service on the 3730xl DNA Analyzer by Applied Biosystems\u2122. Custom projects available upon request.<\/p>\n","protected":false},"author":37,"featured_media":0,"parent":92941,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"template-platforms-services.blade.php","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-92945","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Sanger Sequencing - Microsatellite marker genotyping (MicroSAT) and others - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/sanger-sequencing\/sanger-sequencing-microsatellite-marker-genotyping\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Sanger Sequencing - Microsatellite marker genotyping (MicroSAT) and others - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval\" \/>\n<meta property=\"og:description\" content=\"Microsatellite Electrophoresis Service on the 3730xl DNA Analyzer by Applied Biosystems\u2122. 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