{"id":66594,"date":"2024-06-18T15:13:35","date_gmt":"2024-06-18T19:13:35","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/equipement-orbitrapfusion\/"},"modified":"2024-07-05T14:05:35","modified_gmt":"2024-07-05T18:05:35","slug":"equipement-orbitrapfusion","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/equipement-orbitrapfusion\/","title":{"rendered":"Orbitrap Fusion\u202f- U3000"},"content":{"rendered":"<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Director<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Arnaud Droit, Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Coordinator<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Florence Roux-Dalvai, M.Sc.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Proteomics<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        2705 boul. Laurier, local R2-2710<br \/>\r\nQu\u00e9bec (Qu\u00e9bec) Canada G1V 4G2\n                                    <\/div>\n                                \n                                                                    <div class=\"mb-1\">\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2261\">+1 418-654-2261<\/a>\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2159 (fax)\">+1 418-654-2159 (fax)<\/a>\n                                                                            <\/div>\n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:prot\u00e9omique@crchudequebec.ulaval.ca\" title=\"prot\u00e9omique@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">prot\u00e9omique@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                            \n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                                            <figure class=\"md:mb-12 mb-6\">\n                            <img loading=\"lazy\" decoding=\"async\" width=\"697\" height=\"345\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Instruments_Fusion.png\" class=\"rounded-3xl\" alt=\"\" srcset=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Instruments_Fusion.png 697w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Instruments_Fusion-300x148.png 300w\" sizes=\"auto, (max-width: 697px) 100vw, 697px\" \/>\n\n                                                    <\/figure>\n                    \n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p><span data-contrast=\"auto\">The Orbitrap Fusion Tribrid (Thermo Fisher Scientific) is a mass spectrometer that combines three different analyzers: a quadrupole (enabling efficient ion selection), a linear trap (allowing rapid detection of fragmentation spectra), and an Orbitrap (providing very precise mass measurement with ultra-high resolution up to 450K).<\/span><span data-ccp-props=\"{&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335559739&quot;:120,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"auto\">In addition to the classical CID fragmentation, this mass spectrometer is also capable of performing other types of peptide fragmentation thanks to its HCD cell (particularly useful for TMT quantifications) and its ETD source, which is more efficient for identifying post-translational modifications such as phosphorylations, glycosylations, etc.<\/span><span data-ccp-props=\"{&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335559739&quot;:120,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"auto\">This instrument is coupled with an ultra-high-pressure liquid chromatography system (up to 800 bars) U3000 RSLC nano (Thermo Fisher Scientific) and a FAIMS Pro ion mobility system allowing background noise reduction in spectra.<\/span><span data-ccp-props=\"{&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335559739&quot;:120,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"auto\">Although it is an older instrument compared to others in the platform, its precision and versatility make the Orbitrap Fusion an instrument of choice for the analysis of post-translational modifications (PTMs) or protein complexes (immunoprecipitations, pull-downs).<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":10,"featured_media":0,"parent":0,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-66594","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Orbitrap Fusion\u202f- U3000 - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.crchudequebec.ulaval.ca\/en\/equipement-orbitrapfusion\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta 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