{"id":66517,"date":"2024-05-14T13:57:51","date_gmt":"2024-05-14T17:57:51","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/genomics-center\/proteomique\/proteomique-ciblee\/"},"modified":"2024-07-05T11:59:02","modified_gmt":"2024-07-05T15:59:02","slug":"proteomique-ciblee","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/proteomique-ciblee\/","title":{"rendered":"Targeted Proteomics"},"content":{"rendered":"<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Director<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Arnaud Droit, Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Coordinator<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Florence Roux-Dalvai, M.Sc.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Proteomics<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        2705 boul. Laurier, local R2-2710<br \/>\r\nQu\u00e9bec (Qu\u00e9bec) Canada G1V 4G2\n                                    <\/div>\n                                \n                                                                    <div class=\"mb-1\">\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2261\">+1 418-654-2261<\/a>\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2159 (fax)\">+1 418-654-2159 (fax)<\/a>\n                                                                            <\/div>\n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:prot\u00e9omique@crchudequebec.ulaval.ca\" title=\"prot\u00e9omique@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">prot\u00e9omique@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                            \n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                    \n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p><span class=\"TextRun SCXW72540561 BCX0\" lang=\"FR-FR\" xml:lang=\"FR-FR\" data-contrast=\"none\"><span class=\"NormalTextRun SCXW72540561 BCX0\">In <\/span><span class=\"NormalTextRun ContextualSpellingAndGrammarErrorV2Themed SCXW72540561 BCX0\">cases<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">where<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">we<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> are <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">interested<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> in a <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">limited<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">number<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> of <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">proteins<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">within<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">complex<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">proteomes<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\">, <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">it<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">is<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> possible to <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">perform<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> a <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">targeted<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">analysis<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> of <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">these<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">proteins<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\">. <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">We<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> use the PRM (<\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">Parallel<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">Reaction<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> Monitoring) <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">method<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">based<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> on high-<\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">resolution<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> mass <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">spectrometry<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\">, <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">which<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">reduces<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> the <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">risk<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> of <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">interference<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\">. <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">During<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">this<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">analysis<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\">, the mass <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">spectrometer<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">specifically<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">filters<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> peptides <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">corresponding<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> to the <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">proteins<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> of <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">interest<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> and fragments <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">them<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> to <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">obtain<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> MS2-type <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">spectra<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\">. The <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">most<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> intense fragments of <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">each<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">spectrum<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> are <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">extracted<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> to <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">reconstruct<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> the <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">elution<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">peak<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> of the <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">corresponding<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> peptide and <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">obtain<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> quantification by <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">integrating<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> the area <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">under<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\"> the <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW72540561 BCX0\">peak<\/span><span class=\"NormalTextRun SCXW72540561 BCX0\">.<\/span><\/span><span class=\"EOP SCXW72540561 BCX0\" data-ccp-props=\"{&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559739&quot;:120,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-65194\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Proteomique_Services_ProteomiqueCiblee-e1718800888689.png\" alt=\"\" width=\"800\" height=\"181\" \/><\/p>\n<p><span data-contrast=\"none\">About a hundred proteins can be monitored simultaneously in a single analysis.<\/span><span data-ccp-props=\"{&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559739&quot;:120,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">This method allows for the validation of potential biomarkers identified during global proteomic analyses. It is also an alternative to Western blot when antibodies are not available or when a large number of samples need to be analyzed.<\/span><span data-ccp-props=\"{&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559739&quot;:120,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">Signal normalization is done by adding an internal standard (cytochrome C digest) or isotopically labeled synthetic peptides.\u00a0<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">The use of high-purity synthetic peptides can also provide an absolute quantification of the protein in the sample (\u00b5g\/mL) and not just a relative quantification between several samples.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Targeted proteomics analysis provides relative or absolute quantification of proteins of interest in each sample. <\/p>\n","protected":false},"author":10,"featured_media":0,"parent":46835,"menu_order":8,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-66517","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - 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