{"id":66495,"date":"2024-02-21T11:56:28","date_gmt":"2024-02-21T16:56:28","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/"},"modified":"2024-07-09T12:09:39","modified_gmt":"2024-07-09T16:09:39","slug":"preparation-dechantillons","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/","title":{"rendered":"Sample Preparation"},"content":{"rendered":"\n

<\/p>\n\n\n

\n
\n
\n
\n
\n
\n \n \n
\n

Director<\/h3>\n \n
\n Arnaud Droit, Ph.D\n <\/div>\n \n \n \n <\/div>\n
\n

Coordinator<\/h3>\n \n
\n Florence Roux-Dalvai, M.Sc\n <\/div>\n \n \n \n <\/div>\n
\n

Proteomics<\/h3>\n \n
\n Plateforme de prot\u00e9omique 2705 boul. Laurier, local R2-2710
\r\nQu\u00e9bec (Qu\u00e9bec) Canada G1V 4G2
\r\n\n <\/div>\n \n
\n \n 1 418-654-2261<\/a>\n \n 1 418-654-2159 (fax)<\/a>\n <\/div>\n \n \n <\/div>\n \n \n \n \n <\/div>\n
\n \n \n
\n
\n

Extraction<\/span><\/b>\u00a0<\/span><\/h4>\n

All types of biological samples are analyzable by mass spectrometry provided that proteins can be extracted from them.<\/span>\u00a0<\/span><\/p>\n

Our staff are experts in extracting proteins from a wide variety of samples (tissues, cells, biological fluids, culture media, etc.) and organisms (human, mammalian, plant, microorganisms, etc.).\u00a0 <\/span>\u00a0<\/span><\/p>\n

We use mechanical extraction methods (grinding, sonication, bead beating, etc.) or chemical methods (detergents, solvents, etc.).<\/span>\u00a0<\/span><\/p>\n

We adapt our protocols to your project.<\/span>\u00a0<\/span><\/p>\n

After extraction, proteins are denatured, usually by heating, and we also proceed with reduction and alkylation of disulfide bridges to fully unfold the proteins.<\/span>\u00a0<\/span> \u00a0<\/span><\/p>\n

\"\"<\/p>\n

Enzymatic<\/span> Digestion<\/span> <\/span><\/h4>\n

The type of analysis we perform is called “Bottom-Up Proteomics.” This strategy involves digesting the extracted proteins with an enzyme. The resulting peptides are then injected into the mass spectrometer. In most cases, we use trypsin, which cleaves after basic amino acids (lysine and arginine), thus generating peptides of suitable size and easily ionizable in the instrument’s source.<\/span>\u00a0<\/span><\/p>\n

It is also possible to directly digest proteins present in an SDS-PAGE gel band or immobilized on beads (immunoprecipitation, pull-down).<\/span>\u00a0<\/span><\/p>\n

Protein<\/span> or Peptide <\/span>Assay<\/span><\/span>\u00a0<\/span><\/h4>\n

To <\/span>verify<\/span> if <\/span>your<\/span> samples<\/span> contain<\/span> sufficient<\/span> proteins<\/span> for <\/span>analysis<\/span>, <\/span>we<\/span> can <\/span>perform<\/span> protein<\/span> assays<\/span> after<\/span> extraction (Bradford <\/span>method<\/span>) or peptide <\/span>assays<\/span> after<\/span> digestion (<\/span>nanodrop<\/span> assay<\/span> at 205nm). This <\/span>step<\/span> also<\/span> allows<\/span> for <\/span>analysis<\/span> normalization<\/span> by <\/span>injecting<\/span> identical<\/span> amounts<\/span> of <\/span>different<\/span> samples<\/span> into<\/span> the mass <\/span>spectrometer<\/span>.<\/span><\/span>\u00a0<\/span><\/p>\n

Peptide Purification<\/span><\/span> <\/span><\/h4>\n

The peptides must then be purified before injection. For this purpose, we use Solid Phase Extraction (SPE) method on C18 beads, which <\/span>retain<\/span> peptides based on hydrophobicity.<\/span><\/span>\u00a0<\/span><\/p>\n

Other<\/span> Preparations<\/span><\/h4>\n

Depending<\/span> on the <\/span>desired<\/span> analysis<\/span> type, <\/span>it<\/span> is<\/span> also<\/span> possible to <\/span>perform<\/span> other<\/span> sample<\/span> preparation<\/span> steps<\/span>:<\/span><\/span>\u00a0<\/span><\/p>\n

    \n
  • Concentration by ultrafiltration<\/span>\u00a0<\/span><\/li>\n
  • P<\/span>ost-<\/span>translationnal<\/span> modifications (PTMs)<\/span> enrichment<\/span><\/span>\u00a0<\/span><\/li>\n
  • Deglycosylation<\/span><\/span>\u00a0<\/span><\/li>\n
  • TMT <\/span>labeling<\/span><\/li>\n
  • High pH <\/span>fractionation<\/span><\/li>\n
  • Etc.<\/li>\n<\/ul>\n\n <\/div>\n <\/div>\n \n \n \n \n <\/div>\n <\/div>\n <\/div>\n <\/div>\n<\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"

    The proteomics platform can handle all stages of sample preparation prior to analysis (extraction, assay, digestion, enrichment of PTMs, etc.). <\/p>\n","protected":false},"author":6,"featured_media":0,"parent":46835,"menu_order":13,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-66495","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nSample Preparation - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Sample Preparation - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval\" \/>\n<meta property=\"og:description\" content=\"The proteomics platform can handle all stages of sample preparation prior to analysis (extraction, assay, digestion, enrichment of PTMs, etc.).\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/\" \/>\n<meta property=\"og:site_name\" content=\"Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/crchuqc\" \/>\n<meta property=\"article:modified_time\" content=\"2024-07-09T16:09:39+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@crchuqc\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/\",\"url\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/\",\"name\":\"Sample Preparation - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval\",\"isPartOf\":{\"@id\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/#website\"},\"datePublished\":\"2024-02-21T16:56:28+00:00\",\"dateModified\":\"2024-07-09T16:09:39+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Platforms and Services\",\"item\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/plateformes-et-services\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Genomics Center\",\"item\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/plateformes-et-services\/genomique\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"Proteomics\",\"item\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/plateformes-et-services\/genomique\/proteomique\/\"},{\"@type\":\"ListItem\",\"position\":5,\"name\":\"Sample Preparation\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/#website\",\"url\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/\",\"name\":\"Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/#organization\",\"name\":\"Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval\",\"url\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/04\/CRCHU-Logo-LinkedIn-blanc.png\",\"contentUrl\":\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/04\/CRCHU-Logo-LinkedIn-blanc.png\",\"width\":1200,\"height\":1200,\"caption\":\"Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval\"},\"image\":{\"@id\":\"https:\/\/www.crchudequebec.ulaval.ca\/en\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.facebook.com\/crchuqc\",\"https:\/\/x.com\/crchuqc\",\"https:\/\/www.linkedin.com\/company\/crchuq\/\",\"https:\/\/bsky.app\/profile\/crchudequebec.bsky.social\",\"https:\/\/www.youtube.com\/@crchudequebec8404\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Sample Preparation - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/","og_locale":"en_US","og_type":"article","og_title":"Sample Preparation - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval","og_description":"The proteomics platform can handle all stages of sample preparation prior to analysis (extraction, assay, digestion, enrichment of PTMs, etc.).","og_url":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/","og_site_name":"Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval","article_publisher":"https:\/\/www.facebook.com\/crchuqc","article_modified_time":"2024-07-09T16:09:39+00:00","twitter_card":"summary_large_image","twitter_site":"@crchuqc","schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/","url":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/","name":"Sample Preparation - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval","isPartOf":{"@id":"https:\/\/www.crchudequebec.ulaval.ca\/en\/#website"},"datePublished":"2024-02-21T16:56:28+00:00","dateModified":"2024-07-09T16:09:39+00:00","breadcrumb":{"@id":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/preparation-dechantillons\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/www.crchudequebec.ulaval.ca\/en\/"},{"@type":"ListItem","position":2,"name":"Platforms and Services","item":"https:\/\/www.crchudequebec.ulaval.ca\/en\/plateformes-et-services\/"},{"@type":"ListItem","position":3,"name":"Genomics Center","item":"https:\/\/www.crchudequebec.ulaval.ca\/en\/plateformes-et-services\/genomique\/"},{"@type":"ListItem","position":4,"name":"Proteomics","item":"https:\/\/www.crchudequebec.ulaval.ca\/en\/plateformes-et-services\/genomique\/proteomique\/"},{"@type":"ListItem","position":5,"name":"Sample Preparation"}]},{"@type":"WebSite","@id":"https:\/\/www.crchudequebec.ulaval.ca\/en\/#website","url":"https:\/\/www.crchudequebec.ulaval.ca\/en\/","name":"Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval","description":"","publisher":{"@id":"https:\/\/www.crchudequebec.ulaval.ca\/en\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.crchudequebec.ulaval.ca\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.crchudequebec.ulaval.ca\/en\/#organization","name":"Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval","url":"https:\/\/www.crchudequebec.ulaval.ca\/en\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.crchudequebec.ulaval.ca\/en\/#\/schema\/logo\/image\/","url":"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/04\/CRCHU-Logo-LinkedIn-blanc.png","contentUrl":"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/04\/CRCHU-Logo-LinkedIn-blanc.png","width":1200,"height":1200,"caption":"Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval"},"image":{"@id":"https:\/\/www.crchudequebec.ulaval.ca\/en\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/crchuqc","https:\/\/x.com\/crchuqc","https:\/\/www.linkedin.com\/company\/crchuq\/","https:\/\/bsky.app\/profile\/crchudequebec.bsky.social","https:\/\/www.youtube.com\/@crchudequebec8404"]}]}},"_links":{"self":[{"href":"https:\/\/www.crchudequebec.ulaval.ca\/en\/wp-json\/wp\/v2\/pages\/66495","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.crchudequebec.ulaval.ca\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.crchudequebec.ulaval.ca\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.crchudequebec.ulaval.ca\/en\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.crchudequebec.ulaval.ca\/en\/wp-json\/wp\/v2\/comments?post=66495"}],"version-history":[{"count":2,"href":"https:\/\/www.crchudequebec.ulaval.ca\/en\/wp-json\/wp\/v2\/pages\/66495\/revisions"}],"predecessor-version":[{"id":66851,"href":"https:\/\/www.crchudequebec.ulaval.ca\/en\/wp-json\/wp\/v2\/pages\/66495\/revisions\/66851"}],"up":[{"embeddable":true,"href":"https:\/\/www.crchudequebec.ulaval.ca\/en\/wp-json\/wp\/v2\/pages\/46835"}],"wp:attachment":[{"href":"https:\/\/www.crchudequebec.ulaval.ca\/en\/wp-json\/wp\/v2\/media?parent=66495"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}