{"id":66447,"date":"2024-05-14T13:48:09","date_gmt":"2024-05-14T17:48:09","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/genomics-center\/proteomique\/analyse-proteomique\/"},"modified":"2024-07-05T09:55:06","modified_gmt":"2024-07-05T13:55:06","slug":"analyse-proteomique","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/proteomique\/analyse-proteomique\/","title":{"rendered":"Proteomic analysis"},"content":{"rendered":"<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Director<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Arnaud Droit, Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Coordinator<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Florence Roux-Dalvai, M.Sc.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Proteomics<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        2705 boul. Laurier, local R2-2710<br \/>\r\nQu\u00e9bec (Qu\u00e9bec) Canada G1V 4G2\n                                    <\/div>\n                                \n                                                                    <div class=\"mb-1\">\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2261\">+1 418-654-2261<\/a>\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-654-2159 (fax)\">+1 418-654-2159 (fax)<\/a>\n                                                                            <\/div>\n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:prot\u00e9omique@crchudequebec.ulaval.ca\" title=\"prot\u00e9omique@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">prot\u00e9omique@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                            \n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                    \n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p><span class=\"TextRun SCXW89301306 BCX0\" lang=\"EN-US\" xml:lang=\"EN-US\" data-contrast=\"none\"><span class=\"NormalTextRun SCXW89301306 BCX0\">The <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">proteome<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">is<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">defined<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> as the set of <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">proteins<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">expressed<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> in an <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">organism<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">, tissue, <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">cell<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">, <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">biological<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">fluid<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">, organelle <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">under<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">given<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> conditions and at a <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">given<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> time (Mark Wilkins, 1994). <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">Unlike<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> the <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">genome<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">, the <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">proteome<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">is<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">dynamic<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">, and <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">its<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">study<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">elucidates<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">many<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">biological<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">processes<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">. It <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">is<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> important to note <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">that<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">proteins<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> are direct <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">products<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> of <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">genes<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">, <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">that<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> a <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">genetic<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">sequence<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> can <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">produce<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">several<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">protein<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">products<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">, and <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">each<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> of <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">them<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> can <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">undergo<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">various<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> post-<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">translational<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> modifications. <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">Together<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">, <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">these<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">numerous<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW89301306 BCX0\">proteoforms<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> (<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">estimated<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> to <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">be<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> over a million in <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">humans<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">) <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">reflect<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> cellular <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">function<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\"> and <\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">explain<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">biological<\/span> <span class=\"NormalTextRun SCXW89301306 BCX0\">complexity<\/span><span class=\"NormalTextRun SCXW89301306 BCX0\">.<\/span><\/span><span class=\"EOP SCXW89301306 BCX0\" data-ccp-props=\"{&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335559739&quot;:120,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<figure id=\"attachment_65178\" aria-describedby=\"caption-attachment-65178\" style=\"width: 750px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-65178\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Proteomique_Services_AnalyseProteomique_1.png\" alt=\"\" width=\"750\" height=\"400\" srcset=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Proteomique_Services_AnalyseProteomique_1.png 902w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Proteomique_Services_AnalyseProteomique_1-300x160.png 300w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Proteomique_Services_AnalyseProteomique_1-768x410.png 768w\" sizes=\"auto, (max-width: 750px) 100vw, 750px\" \/><figcaption id=\"caption-attachment-65178\" class=\"wp-caption-text\">Source : thermofisher.com<\/figcaption><\/figure>\n<p><span class=\"TextRun SCXW6980467 BCX0\" lang=\"EN-US\" xml:lang=\"EN-US\" data-contrast=\"none\"><span class=\"NormalTextRun SCXW6980467 BCX0\">Proteomic<\/span> <span class=\"NormalTextRun SCXW6980467 BCX0\">analysis<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\"> by mass <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">spectrometry<\/span> <span class=\"NormalTextRun SCXW6980467 BCX0\">is<\/span> <span class=\"NormalTextRun SCXW6980467 BCX0\">currently<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\"> the <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">most<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\"> effective <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">method<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\"> for <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">obtaining<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\"> maximum information about the <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">proteins<\/span> <span class=\"NormalTextRun SCXW6980467 BCX0\">composing<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\"> a <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">biological<\/span> <span class=\"NormalTextRun SCXW6980467 BCX0\">sample<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">. It can <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">provide<\/span> <span class=\"NormalTextRun SCXW6980467 BCX0\">numerous<\/span> <span class=\"NormalTextRun SCXW6980467 BCX0\">pieces<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\"> of information <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">such<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\"> as <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">protein<\/span> <span class=\"NormalTextRun SCXW6980467 BCX0\">abundance<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">, <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">protein-protein<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\"> interactions or <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">with<\/span> <span class=\"NormalTextRun SCXW6980467 BCX0\">other<\/span> <span class=\"NormalTextRun SCXW6980467 BCX0\">molecules<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">, the <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">presence<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\"> of post-<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">translational<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\"> modifications, <\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">subcellular<\/span> <span class=\"NormalTextRun SCXW6980467 BCX0\">localization<\/span><span class=\"NormalTextRun SCXW6980467 BCX0\">, etc.<\/span><\/span><span class=\"EOP SCXW6980467 BCX0\" data-ccp-props=\"{&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335559739&quot;:120,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<figure id=\"attachment_65180\" aria-describedby=\"caption-attachment-65180\" style=\"width: 751px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-65180\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Proteomique_Services_AnalyseProteomique_2.png\" alt=\"\" width=\"751\" height=\"751\" srcset=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Proteomique_Services_AnalyseProteomique_2.png 895w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Proteomique_Services_AnalyseProteomique_2-300x300.png 300w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Proteomique_Services_AnalyseProteomique_2-150x150.png 150w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/Proteomique_Services_AnalyseProteomique_2-768x768.png 768w\" sizes=\"auto, (max-width: 751px) 100vw, 751px\" \/><figcaption id=\"caption-attachment-65180\" class=\"wp-caption-text\">Source : Zengin et al. MOJ Proteomics Bioinform. 2017;5(3):86-92<\/figcaption><\/figure>\n<p><span data-contrast=\"none\">It is also important to note that unlike gene expression analysis by transcriptomics (RNA seq), proteomics provides access to proteins, which are the final effectors of genes. It has been demonstrated that there is a low correlation between protein expression levels and mRNA levels because the efficiency of translation and the half-life of mRNA and proteins can have a significant impact on the proteome content.\u00a0<\/span><span data-ccp-props=\"{&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335559739&quot;:120,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">By allowing the identification and quantification of thousands of proteins from less than 1\u00b5g of protein extract in less than an hour, proteomic analysis by mass spectrometry has become an essential tool in many fields: biology, diagnostics, microbiology, toxicology, biotechnology, nutrition, etc.<\/span><span data-ccp-props=\"{&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335559739&quot;:120,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Proteomic analysis by mass spectrometry enables the identification and quantification of hundreds or thousands of proteins from all types of biological samples.   <\/p>\n","protected":false},"author":10,"featured_media":0,"parent":46835,"menu_order":12,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-66447","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - 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