{"id":66432,"date":"2024-05-14T07:52:52","date_gmt":"2024-05-14T11:52:52","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/genomics-center\/sequencage-ngs\/"},"modified":"2026-03-23T14:55:00","modified_gmt":"2026-03-23T18:55:00","slug":"next-generation-sequencing","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/next-generation-sequencing\/","title":{"rendered":"Next-generation sequencing (NGS) and high-throughput genotyping"},"content":{"rendered":"<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Director<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Jacques Simard, Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Manager<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Martine Dumont, Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Next-generation sequencing platform<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        2705 Laurier Boulevard<br \/>\r\nOffice R-4784, Delivery R-4720<br \/>\r\nQu\u00e9bec, Qu\u00e9bec\n                                    <\/div>\n                                \n                                                                    <div class=\"mb-1\">\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-525-4444, ext 46406 \/ 42296\">+1 418-525-4444, ext 46406 \/ 42296<\/a>\n                                                                            <\/div>\n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">High-throughput genotyping service (available in 2026)<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        <strong>Manager<\/strong><br \/>\r\nMathias Cavaill\u00e9, MD<br \/>\r\n<a class=\"phone\" href=\"mailto:mathias.cavaill\u00e9@crchudequebec.ulaval.ca\">mathias.cavaill\u00e9@crchudequebec.ulaval.ca<\/a>\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                            \n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                                            <figure class=\"md:mb-12 mb-6\">\n                            <img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"1080\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/MHA_17-090_542-1920x1080.jpg\" class=\"rounded-3xl\" alt=\"\" \/>\n\n                                                    <\/figure>\n                    \n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p>The Next-Generation Sequencing Platform has been offering high-throughput sequencing services on an Illumina infrastructure since 2014. Over the years, it has acquired solid expertise in a wide range of applications in genomics, transcriptomics, and epigenomics. See the details of the services offered in the <a href=\"#NGS_Services_EN\">Our Services section<\/a>.<\/p>\n<p>The sequencing infrastructure is based on Illumina technology, which remains the most widely used technology worldwide. The platform is notably equipped with a NovaSeq 6000, which offers very high sequencing throughput (up to 20 billion paired reads per sequencing cycle) at a very low cost per base, as well as lower throughput sequencing instruments, including the iSeq100 and MiSeq V3. The platform is well complemented by a Biomek FXP automated workstation offering numerous sequencing-related services, including several automated methods for library preparation for various applications, a high-performance Covaris ultrasonicator, and quality control instruments such as a TapeStation 4200 and a Qubit 3.0. Our sequencing infrastructure is a versatile next-generation sequencing platform that can accommodate genomics projects of any scale (from a few samples to several thousand). We also provide sequencing services for libraries prepared by clients as well as quality control services for samples and libraries.<\/p>\n<p>&nbsp;<\/p>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                                            <div class=\"buttons mt-5 flex flex-wrap gap-4\">\n                                                            <a href=\"#NGS_Services_EN\" class=\"btn\" target=\"\">Our Services<\/a>\n                                                            <a href=\"#NGS_Equipements_EN\" class=\"btn\" target=\"\">Our Equipment<\/a>\n                                                            <a href=\"#ngs_demande-EN\" class=\"btn\" target=\"\">Submit a Request<\/a>\n                                                    <\/div>\n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n\n\n<section class=\"bg-white align wp-block-acf-team\">\n    <section class=\"bg-white slider\">\n        <div class=\"padd\">\n            <div class=\"container\">\n                <div class=\"wrap\">\n                    <div class=\"flex relative justify-between items-center sm:pb-14 pb-7\">\n                        <div class=\"w-1\/2\">\n                            <h2 class=\"mb-0\">Team<\/h2>\n                        <\/div>\n                        <div class=\"w-auto\">\n                            <div class=\"flex sm:gap-x-4 gap-x-2.5 arrows\">\n                                <div class=\"swiper-button-prev\"><i class=\"fa-light fa-arrow-left\"><\/i><\/div>\n                                <div class=\"swiper-button-next\"><i class=\"fa-light fa-arrow-right\"><\/i><\/div>\n                            <\/div>\n                        <\/div>\n                    <\/div>\n                <\/div>\n            <\/div>\n\n            <div class=\"fit-l-to-container\">\n                <div class=\"swiper\">\n                    <div class=\"swiper-wrapper\">\n                                                    <div class=\"relative swiper-slide\">\n                                <h3>Martine Dumont<\/h3>\n                                <h4>Manager<\/h4>\n                                <div class=\"flex flex-col\">\n                                                                                                                        <a class=\"phone flex\" href=\"tel:1 418 525-4444, ext. 46406 \/ 42296\"\n                                                title=\"Dial\">1 418 525-4444, ext. 46406 \/ 42296<\/a>\n                                                                            \t\t\t\t                                                                                                                        <a class=\"group flex items-center mt-2\" href=\"mailto:Martine.Dumont@crchudequebec.ulaval.ca\"\n                                                title=\"Write to Martine Dumont (Martine.Dumont@crchudequebec.ulaval.ca)\">\n                                                <i\n                                                    class=\"fa-light fa-envelope shrink-0 mr-2 group-hover:border-blue-900 group-hover:bg-blue-200\/0\"><\/i>\n                                                <span\n                                                    class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500\">Martine.Dumont@crchudequebec.ulaval.ca<\/span>\n                                            <\/a>\n                                                                            \t\t\t\t                                <\/div>\n                            <\/div>\n                                                    <div class=\"relative swiper-slide\">\n                                <h3>St\u00e9phane Dubois<\/h3>\n                                <h4>Research professional<\/h4>\n                                <div class=\"flex flex-col\">\n                                                                                                                        <a class=\"phone flex\" href=\"tel:1 418 654-2296\"\n                                                title=\"Dial\">1 418 654-2296<\/a>\n                                                                            \t\t\t\t                                                                                                                        <a class=\"group flex items-center mt-2\" href=\"mailto:Stephane.Dubois@crchudequebec.ulaval.ca\"\n                                                title=\"Write to St\u00e9phane Dubois (Stephane.Dubois@crchudequebec.ulaval.ca)\">\n                                                <i\n                                                    class=\"fa-light fa-envelope shrink-0 mr-2 group-hover:border-blue-900 group-hover:bg-blue-200\/0\"><\/i>\n                                                <span\n                                                    class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500\">Stephane.Dubois@crchudequebec.ulaval.ca<\/span>\n                                            <\/a>\n                                                                            \t\t\t\t                                <\/div>\n                            <\/div>\n                                                    <div class=\"relative swiper-slide\">\n                                <h3>Annie-Claude Collin-Deschesnes<\/h3>\n                                <h4>Specialized research technician<\/h4>\n                                <div class=\"flex flex-col\">\n                                                                                                                        <a class=\"phone flex\" href=\"tel:1 418 654-2296\"\n                                                title=\"Dial\">1 418 654-2296<\/a>\n                                                                            \t\t\t\t                                                                                                                        <a class=\"group flex items-center mt-2\" href=\"mailto:Annie-C.C-Deschesnes@crchudequebec.ulaval.ca\"\n                                                title=\"Write to Annie-Claude Collin-Deschesnes (Annie-C.C-Deschesnes@crchudequebec.ulaval.ca)\">\n                                                <i\n                                                    class=\"fa-light fa-envelope shrink-0 mr-2 group-hover:border-blue-900 group-hover:bg-blue-200\/0\"><\/i>\n                                                <span\n                                                    class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500\">Annie-C.C-Deschesnes@crchudequebec.ulaval.ca<\/span>\n                                            <\/a>\n                                                                            \t\t\t\t                                <\/div>\n                            <\/div>\n                                                    <div class=\"relative swiper-slide\">\n                                <h3>Ariane Forgues<\/h3>\n                                <h4>Research technician<\/h4>\n                                <div class=\"flex flex-col\">\n                                                                                                                        <a class=\"phone flex\" href=\"tel:1 418 654-2296\"\n                                                title=\"Dial\">1 418 654-2296<\/a>\n                                                                            \t\t\t\t                                                                                                                        <a class=\"group flex items-center mt-2\" href=\"mailto:Ariane.forgues@crchudequebec.ulaval.ca\"\n                                                title=\"Write to Ariane Forgues (Ariane.forgues@crchudequebec.ulaval.ca)\">\n                                                <i\n                                                    class=\"fa-light fa-envelope shrink-0 mr-2 group-hover:border-blue-900 group-hover:bg-blue-200\/0\"><\/i>\n                                                <span\n                                                    class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500\">Ariane.forgues@crchudequebec.ulaval.ca<\/span>\n                                            <\/a>\n                                                                            \t\t\t\t                                <\/div>\n                            <\/div>\n                                            <\/div>\n                    <div class=\"swiper-pagination\"><\/div>\n                <\/div>\n            <\/div>\n        <\/div>\n    <\/section>\n<\/section>\n\n\n<section id=\"NGS_Services_EN\" data-block=\"block_5e84ba9403e42eb8c4feeee0be1cef8a\" class=\"block-accordions align bg-white\">\n    <div class=\"container\">\n        <div class=\"padd\">\n            <div class=\"wrap\">\n                <h2 class=\"lg:mb-14 mb-7 sm:text-48 text-36\">Our Services<\/h2>\n\n    <div class=\"w-full divide-y divide-blue-200 border-blue-200 last:border-b border-t\">\n                                    <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Library preparation for genomics\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <ul>\n<li>Human genome sequencing including FFPE tissue genome<\/li>\n<li>Microbial genome sequencing<\/li>\n<li>Exome sequencing<\/li>\n<li>Targeted gene capture sequencing<\/li>\n<li>Specific PCR amplicon sequencing<\/li>\n<li>Sequencing of ribosomal RNA gene regions 16S (bacteria), ITS (fungi), 18S and other targets with a multiplexing capacity of up to 1152 samples<\/li>\n<li>Quality control service (bioanalysis and fluorescence quantification) for samples and libraries<\/li>\n<\/ul>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Library preparation for transcriptomics\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <ul>\n<li>Total RNA sequencing with RNA quantities ranging from 5 ng to 1000 ng<\/li>\n<li>Total RNA sequencing on FFPE tissues<\/li>\n<li>mRNA sequencing<\/li>\n<li>MicroRNA sequencing<\/li>\n<li>Single-cell transcriptome sequencing<\/li>\n<\/ul>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Library preparation for epigenomics\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <ul>\n<li>ChIP-seq (CHromatin ImmunoPrecipitation)<\/li>\n<li>CUT&amp;RUN (Cleavage Under Targets &amp; Release Using Nuclease)<\/li>\n<li>ATAC-seq (Assay for Transposase Accessible Chromatin)<\/li>\n<li>RRBS (Reduced-representation bisulfite sequencing)<\/li>\n<\/ul>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Sequencing of client-prepared libraries according to the requested sequencing depth\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <ul>\n<li>Multiplexing of libraries with other projects<\/li>\n<li>Library verification option through low-depth sequencing<\/li>\n<li>Sequencing on NovaSeq 6000; read lengths between 100 bp and 250 bp in paired-end format<\/li>\n<li>Sequencing on MiSeq v3 (standard, micro, or nano); read lengths between 100 bp and 300 bp in paired-end format<\/li>\n<\/ul>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                        <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/section>\n\n\n<section id=\"NGS_Equipements_EN\" data-block=\"block_81cf7bd353dcb4be7933cc1958213ae3\" class=\"block-equipment align bg-white\">\n    <div class=\"container\">\n        <div class=\"padd padd--bottom\">\n            <div class=\"wrap\">\n                                    <h2 class=\"mb-9\">Our Equipment<\/h2>\n                \n                <div class=\"flex flex-col gap-y-6\">\n                                            <div class=\"gradient-transition-gray p-6 rounded-3xl\" x-data=\"{ expanded: false }\">\n                            <div class=\"flex sm:flex-row flex-col xl:gap-x-12 gap-x-6\">\n                                <div class=\"lg:w-1\/4 sm:w-5\/12 w-full\">\n                                                                            <figure class=\"overflow-hidden aspect-w-3 aspect-h-1.8 sm:mb-0 mb-4\">\n                                            <img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"1080\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/02\/sequencageNGS_Novaseq-600-1920x1080.jpg\" class=\"rounded-2xl w-full h-full\" alt=\"\" \/>\n                                                                            <\/figure>\n                                <\/div>\n\n                                <div class=\"lg:w-3\/4 sm:w-7\/12 w-full\">\n                                    <div class=\"w-full\">\n                                                                                    <h3 class=\"lg:text-24 lg:mb-5 mb-3 text-18 leading-normal xl:mt-7\">\n                                                Illumina NovaSeq 6000\n                                            <\/h3>\n                                                                                                                            <div class=\"small-text\">\n                                                <p>Data production can reach up to 20 billion (G) paired reads per sequencing cycle.<\/p>\n\n                                            <\/div>\n                                                                                                                            <div class=\"mt-3.5\">\n                                                <a\n                                                    class=\"relative group text-18 font-medium text-blue-600 hover:text-blue-900 duration-300\"\n                                                    href=\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/next-generation-sequencing\/illumina-novaseq-6000\/\"\n                                                    title=\"Read more\"\n                                                    target=\"_blank\"\n                                                >\n                                                Read more&nbsp;<i class=\"group-hover:translate-x-2 fa-solid text-12 fa-arrow-right duration-300 will-change-transform\" aria-hidden=\"true\"><\/i>\n                                                <\/a>\n                                            <\/div>\n                                                                            <\/div>\n\n                                                                    <\/div>\n                            <\/div>\n                        <\/div>\n                                            <div class=\"gradient-transition-gray p-6 rounded-3xl\" x-data=\"{ expanded: false }\">\n                            <div class=\"flex sm:flex-row flex-col xl:gap-x-12 gap-x-6\">\n                                <div class=\"lg:w-1\/4 sm:w-5\/12 w-full\">\n                                                                            <figure class=\"overflow-hidden aspect-w-3 aspect-h-1.8 sm:mb-0 mb-4\">\n                                            <img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"1080\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/02\/sequencageNGS_Misec-Illumina-1920x1080.jpg\" class=\"rounded-2xl w-full h-full\" alt=\"\" \/>\n                                                                            <\/figure>\n                                <\/div>\n\n                                <div class=\"lg:w-3\/4 sm:w-7\/12 w-full\">\n                                    <div class=\"w-full\">\n                                                                                    <h3 class=\"lg:text-24 lg:mb-5 mb-3 text-18 leading-normal xl:mt-7\">\n                                                Illumina MiSeq V3\n                                            <\/h3>\n                                                                                                                            <div class=\"small-text\">\n                                                <p>Data production ranges from 1 to 20 million paired reads per sequencing cycle.<\/p>\n\n                                            <\/div>\n                                                                                                                            <div class=\"mt-3.5\">\n                                                <a\n                                                    class=\"relative group text-18 font-medium text-blue-600 hover:text-blue-900 duration-300\"\n                                                    href=\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/genomics-center\/next-generation-sequencing\/illumina-miseq-v3\/\"\n                                                    title=\"Read more\"\n                                                    target=\"_blank\"\n                                                >\n                                                Read more&nbsp;<i class=\"group-hover:translate-x-2 fa-solid text-12 fa-arrow-right duration-300 will-change-transform\" aria-hidden=\"true\"><\/i>\n                                                <\/a>\n                                            <\/div>\n                                                                            <\/div>\n\n                                                                    <\/div>\n                            <\/div>\n                        <\/div>\n                                            <div class=\"gradient-transition-gray p-6 rounded-3xl\" x-data=\"{ expanded: false }\">\n                            <div class=\"flex sm:flex-row flex-col xl:gap-x-12 gap-x-6\">\n                                <div class=\"lg:w-1\/4 sm:w-5\/12 w-full\">\n                                                                            <figure class=\"overflow-hidden aspect-w-3 aspect-h-1.8 sm:mb-0 mb-4\">\n                                            <img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"1080\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/02\/sequencageNGS_Agilent-1920x1080.jpg\" class=\"rounded-2xl w-full h-full\" alt=\"\" \/>\n                                                                            <\/figure>\n                                <\/div>\n\n                                <div class=\"lg:w-3\/4 sm:w-7\/12 w-full\">\n                                    <div class=\"w-full\">\n                                                                                    <h3 class=\"lg:text-24 lg:mb-5 mb-3 text-18 leading-normal xl:mt-7\">\n                                                Illumina iSeq100 sequencer\n                                            <\/h3>\n                                                                                                                            <div class=\"small-text\">\n                                                <p>Sequencing similar to NovaSeq. Total production of 4 million paired reads. Allows for library recalibration before deep sequencing.<\/p>\n\n                                            <\/div>\n                                                                                                                    <\/div>\n\n                                                                    <\/div>\n                            <\/div>\n                        <\/div>\n                                            <div class=\"gradient-transition-gray p-6 rounded-3xl\" x-data=\"{ expanded: false }\">\n                            <div class=\"flex sm:flex-row flex-col xl:gap-x-12 gap-x-6\">\n                                <div class=\"lg:w-1\/4 sm:w-5\/12 w-full\">\n                                                                            <figure class=\"overflow-hidden aspect-w-3 aspect-h-1.8 sm:mb-0 mb-4\">\n                                            <img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"1080\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/02\/sequencageNGS_Biomek-1920x1080.jpg\" class=\"rounded-2xl w-full h-full\" alt=\"\" \/>\n                                                                            <\/figure>\n                                <\/div>\n\n                                <div class=\"lg:w-3\/4 sm:w-7\/12 w-full\">\n                                    <div class=\"w-full\">\n                                                                                    <h3 class=\"lg:text-24 lg:mb-5 mb-3 text-18 leading-normal xl:mt-7\">\n                                                Biomek FXP automated workstation\n                                            <\/h3>\n                                                                                                                            <div class=\"small-text\">\n                                                <p>Biomek FXP automated workstation offering numerous sequencing-related services, including several automated methods for library preparation<\/p>\n\n                                            <\/div>\n                                                                                                                    <\/div>\n\n                                                                    <\/div>\n                            <\/div>\n                        <\/div>\n                                            <div class=\"gradient-transition-gray p-6 rounded-3xl\" x-data=\"{ expanded: false }\">\n                            <div class=\"flex sm:flex-row flex-col xl:gap-x-12 gap-x-6\">\n                                <div class=\"lg:w-1\/4 sm:w-5\/12 w-full\">\n                                                                            <figure class=\"overflow-hidden aspect-w-3 aspect-h-1.8 sm:mb-0 mb-4\">\n                                            <img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"1080\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/02\/sequencageNGS_Covaris-1920x1080.jpg\" class=\"rounded-2xl w-full h-full\" alt=\"\" \/>\n                                                                            <\/figure>\n                                <\/div>\n\n                                <div class=\"lg:w-3\/4 sm:w-7\/12 w-full\">\n                                    <div class=\"w-full\">\n                                                                                    <h3 class=\"lg:text-24 lg:mb-5 mb-3 text-18 leading-normal xl:mt-7\">\n                                                Covaris ultrasonicator\n                                            <\/h3>\n                                                                                                                            <div class=\"small-text\">\n                                                <p>DNA fragmentation<\/p>\n\n                                            <\/div>\n                                                                                                                    <\/div>\n\n                                                                    <\/div>\n                            <\/div>\n                        <\/div>\n                                            <div class=\"gradient-transition-gray p-6 rounded-3xl\" x-data=\"{ expanded: false }\">\n                            <div class=\"flex sm:flex-row flex-col xl:gap-x-12 gap-x-6\">\n                                <div class=\"lg:w-1\/4 sm:w-5\/12 w-full\">\n                                                                            <figure class=\"overflow-hidden aspect-w-3 aspect-h-1.8 sm:mb-0 mb-4\">\n                                            <img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"640\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/sequencageNGS_PippinPrep-rotated.jpg\" class=\"rounded-2xl w-full h-full\" alt=\"\" srcset=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/sequencageNGS_PippinPrep-rotated.jpg 480w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/sequencageNGS_PippinPrep-225x300.jpg 225w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/>\n                                                                            <\/figure>\n                                <\/div>\n\n                                <div class=\"lg:w-3\/4 sm:w-7\/12 w-full\">\n                                    <div class=\"w-full\">\n                                                                                    <h3 class=\"lg:text-24 lg:mb-5 mb-3 text-18 leading-normal xl:mt-7\">\n                                                Pippin Prep\n                                            <\/h3>\n                                                                                                                            <div class=\"small-text\">\n                                                <p>Allows for the selection of DNA fragments of precise size.<\/p>\n\n                                            <\/div>\n                                                                                                                    <\/div>\n\n                                                                    <\/div>\n                            <\/div>\n                        <\/div>\n                                            <div class=\"gradient-transition-gray p-6 rounded-3xl\" x-data=\"{ expanded: false }\">\n                            <div class=\"flex sm:flex-row flex-col xl:gap-x-12 gap-x-6\">\n                                <div class=\"lg:w-1\/4 sm:w-5\/12 w-full\">\n                                                                            <figure class=\"overflow-hidden aspect-w-3 aspect-h-1.8 sm:mb-0 mb-4\">\n                                            <img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"1080\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/02\/sequencageNGS_Agilent-1920x1080.jpg\" class=\"rounded-2xl w-full h-full\" alt=\"\" \/>\n                                                                            <\/figure>\n                                <\/div>\n\n                                <div class=\"lg:w-3\/4 sm:w-7\/12 w-full\">\n                                    <div class=\"w-full\">\n                                                                                    <h3 class=\"lg:text-24 lg:mb-5 mb-3 text-18 leading-normal xl:mt-7\">\n                                                TapeStation 4200 (quality control system)\n                                            <\/h3>\n                                                                                                                            <div class=\"small-text\">\n                                                <p>Quality verification of starting material and libraries.<\/p>\n\n                                            <\/div>\n                                                                                                                    <\/div>\n\n                                                                    <\/div>\n                            <\/div>\n                        <\/div>\n                                            <div class=\"gradient-transition-gray p-6 rounded-3xl\" x-data=\"{ expanded: false }\">\n                            <div class=\"flex sm:flex-row flex-col xl:gap-x-12 gap-x-6\">\n                                <div class=\"lg:w-1\/4 sm:w-5\/12 w-full\">\n                                                                            <figure class=\"overflow-hidden aspect-w-3 aspect-h-1.8 sm:mb-0 mb-4\">\n                                            <img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"1080\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/02\/sequencageNGS_introvigen-1920x1080.jpg\" class=\"rounded-2xl w-full h-full\" alt=\"\" \/>\n                                                                            <\/figure>\n                                <\/div>\n\n                                <div class=\"lg:w-3\/4 sm:w-7\/12 w-full\">\n                                    <div class=\"w-full\">\n                                                                                    <h3 class=\"lg:text-24 lg:mb-5 mb-3 text-18 leading-normal xl:mt-7\">\n                                                Qubit 3.0\n                                            <\/h3>\n                                                                                                                            <div class=\"small-text\">\n                                                <p>Precise quantification based on DNA\/RNA fluorescence.<\/p>\n\n                                            <\/div>\n                                                                                                                    <\/div>\n\n                                                                    <\/div>\n                            <\/div>\n                        <\/div>\n                                            <div class=\"gradient-transition-gray p-6 rounded-3xl\" x-data=\"{ expanded: false }\">\n                            <div class=\"flex sm:flex-row flex-col xl:gap-x-12 gap-x-6\">\n                                <div class=\"lg:w-1\/4 sm:w-5\/12 w-full\">\n                                                                            <figure class=\"overflow-hidden aspect-w-3 aspect-h-1.8 sm:mb-0 mb-4\">\n                                            <img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"1080\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2025\/02\/sequencageNGS_10xGenomics-1920x1080.jpg\" class=\"rounded-2xl w-full h-full\" alt=\"\" \/>\n                                                                            <\/figure>\n                                <\/div>\n\n                                <div class=\"lg:w-3\/4 sm:w-7\/12 w-full\">\n                                    <div class=\"w-full\">\n                                                                                    <h3 class=\"lg:text-24 lg:mb-5 mb-3 text-18 leading-normal xl:mt-7\">\n                                                Chromium Controller (10X Genomics)\n                                            <\/h3>\n                                                                                                                            <div class=\"small-text\">\n                                                <p>Enables single-cell transcriptome sequencing in complex environments.<\/p>\n\n                                            <\/div>\n                                                                                                                    <\/div>\n\n                                                                    <\/div>\n                            <\/div>\n                        <\/div>\n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/section>\n\n\n<section id=\"ngs_demande-EN\" data-block=\"block_ff56fca255b63bfbfbe6324c6068a7f0\" class=\"block-accordions align bg-white\">\n    <div class=\"container\">\n        <div class=\"padd\">\n            <div class=\"wrap\">\n                <h2 class=\"lg:mb-14 mb-7 sm:text-48 text-36\">Submit a Request<\/h2>\n\n    <div class=\"w-full divide-y divide-blue-200 border-blue-200 last:border-b border-t\">\n                                    <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Pricing\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>Costs for each service are based on the number of samples and sequencing requirements. To request a quote, please send a brief description of your project and\/or needs to <a href=\"mailto:ngs@crchudequebec.ulaval.ca\" rel=\"noreferrer\">ngs@crchudequebec.ulaval.ca<\/a>, attention Martine Dumont. The service cost includes sample demultiplexing and data transfer to the client. For specific bioinformatics analyses, please refer to the bioinformatics platform. For nucleic acid extraction needs, please refer to the gene expression platform.<\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Service Turnaround Time\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>The turnaround time for the service may vary depending on the time of year. Generally, from sample receipt to sequencing data transfer, please allow 4 to 6 weeks. Samples will be stored for up to 2 years. Please let us know if you wish to retrieve them. After this period, they will be destroyed.<\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                     Sample Submission\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <div class=\"flex flex-grow flex-col max-w-full\">\n<div class=\"min-h-[20px] text-message flex flex-col items-start whitespace-pre-wrap break-words [.text-message+&amp;]:mt-5 juice:w-full juice:items-end overflow-x-auto gap-2\" dir=\"auto\" data-message-author-role=\"assistant\" data-message-id=\"841948a0-6348-4d80-9d38-5545ef4a1a82\">\n<div class=\"flex w-full flex-col gap-1 juice:empty:hidden juice:first:pt-[3px]\">\n<div class=\"markdown prose w-full break-words dark:prose-invert dark\">\n<p>To submit your samples, please refer to the volume and concentration tables. For any questions, feel free to contact St\u00e9phane, Annie-Claude, or Ariane at <a href=\"mailto:ngs@crchudequebec.ulaval.ca\" rel=\"noreferrer\">ngs@crchudequebec.ulaval.ca<\/a>. Then, complete the sample submission form and send it to <a href=\"mailto:ngs@crchudequebec.ulaval.ca\" rel=\"noreferrer\">ngs@crchudequebec.ulaval.ca<\/a>.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Sample Submission Form\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p><a href=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2026\/03\/Formulaire-soumission-dechantillons-RA-001-F02.11.xlsx\">Sample Submission Form (FR)<\/a><\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Required Volume and Concentration\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <h5>DNA Libraries<\/h5>\n<table style=\"border-collapse: collapse; width: 100%; height: 312px;\">\n<thead>\n<tr style=\"height: 24px;\">\n<td style=\"width: 20%; height: 24px;\">Library Type<\/td>\n<td style=\"width: 20%; height: 24px;\">Quality<\/td>\n<td style=\"width: 20%; height: 24px;\">Concentration<\/td>\n<td style=\"width: 20%; height: 24px;\">Amount Required for Preparation<\/td>\n<td style=\"width: 20%; height: 24px;\">Volume<\/td>\n<\/tr>\n<\/thead>\n<tbody>\n<tr style=\"height: 18px;\">\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">Genome\/Small Genome<\/td>\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">Free RNA<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">5-200 ng\/\u00b5L<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">\u2265 1 ng<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">20 \u00b5L<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">DIN \u2265 3<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">A260\/A280= 1.8-2.0<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl18\" style=\"width: 20%; height: 18px;\">A260\/A230= 2-2.2<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">CUT &amp; RUN \/ ChIP-seq<\/td>\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">Free RNA<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">&gt;0.1 ng\/\u00b5L<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">\u2265 1 ng<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">20 \u00b5L<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">DIN \u2265 3<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">A260\/A280= 1.8-2.0<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl18\" style=\"width: 20%; height: 18px;\">A260\/A230= 2-2.2<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">16S-ITS<\/td>\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">Free RNA<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">5-50 ng\/\u00b5L<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">\u2265 1 ng<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">20 \u00b5L<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">DIN \u2265 3<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">A260\/A280= 1.8-2.0<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl18\" style=\"width: 20%; height: 18px;\">A260\/A230= 2-2.2<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">Exome, Custom Capture Target<\/td>\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">Free RNA<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">15-100 ng\/\u00b5L<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">\u2265 1 ng<\/td>\n<td style=\"width: 20%; height: 72px;\" rowspan=\"4\">20-50 \u00b5L<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">DIN \u2265 3<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl16\" style=\"width: 20%; height: 18px;\">A260\/A280= 1.8-2.0<\/td>\n<\/tr>\n<tr style=\"height: 18px;\">\n<td class=\"xl18\" style=\"width: 20%; height: 18px;\">A260\/A230= 2-2.2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 10pt;\">* For optimal results, please provide as much material as possible.<\/span><\/p>\n<h5>RNA Libraries<\/h5>\n<table style=\"border-collapse: collapse; width: 100%; height: 216px;\">\n<thead>\n<tr style=\"height: 24px;\">\n<td style=\"width: 20%; height: 24px;\">Library Type<\/td>\n<td style=\"width: 20%; height: 24px;\">Quality<\/td>\n<td style=\"width: 20%; height: 24px;\">Concentration<\/td>\n<td style=\"width: 20%; height: 24px;\">Amount Required for Preparation<\/td>\n<td style=\"width: 20%; height: 24px;\">Volume<\/td>\n<\/tr>\n<\/thead>\n<tbody>\n<tr style=\"height: 24px;\">\n<td style=\"width: 20%; height: 48px;\" rowspan=\"2\">mRNA with Poly-A<\/td>\n<td style=\"width: 20%; height: 24px;\">Free DNA<\/td>\n<td style=\"width: 20%; height: 48px;\" rowspan=\"2\">20-100 ng\/\u00b5L<\/td>\n<td style=\"width: 20%; height: 48px;\" rowspan=\"2\">\u2265 100 ng<\/td>\n<td style=\"width: 20%; height: 48px;\" rowspan=\"2\">20-50 \u00b5L<\/td>\n<\/tr>\n<tr style=\"height: 24px;\">\n<td style=\"width: 20%; height: 24px;\">RIN \u2265 7<\/td>\n<\/tr>\n<tr style=\"height: 24px;\">\n<td style=\"width: 20%; height: 48px;\" rowspan=\"2\">Total RNA<\/td>\n<td style=\"width: 20%; height: 24px;\">Free DNA<\/td>\n<td style=\"width: 20%; height: 48px;\" rowspan=\"2\">20-100 ng\/\u00b5L<\/td>\n<td style=\"width: 20%; height: 48px;\" rowspan=\"2\">\u2265 100 ng<\/td>\n<td style=\"width: 20%; height: 48px;\" rowspan=\"2\">20-50 \u00b5L<\/td>\n<\/tr>\n<tr style=\"height: 24px;\">\n<td style=\"width: 20%; height: 24px;\">RIN \u2265 3 or DV200&gt;70%<\/td>\n<\/tr>\n<tr style=\"height: 24px;\">\n<td style=\"width: 20%; height: 48px;\" rowspan=\"2\">mRNA Low Input<\/td>\n<td style=\"width: 20%; height: 24px;\">Free DNA<\/td>\n<td style=\"width: 20%; height: 24px;\" rowspan=\"2\">Contact Platform<\/td>\n<td style=\"width: 20%; height: 24px;\" rowspan=\"2\">\u2265 1 ng<\/td>\n<td style=\"width: 20%; height: 24px;\" rowspan=\"2\">20-50 \u00b5L<\/td>\n<\/tr>\n<tr style=\"height: 24px;\">\n<td style=\"width: 20%; height: 24px;\">RIN \u2265 7<\/td>\n<\/tr>\n<tr style=\"height: 24px;\">\n<td style=\"width: 20%; height: 48px;\" rowspan=\"2\">Small, Micro RNA<\/td>\n<td style=\"width: 20%; height: 24px;\">Free DNA<\/td>\n<td style=\"width: 20%; height: 24px;\" rowspan=\"2\">100-500 ng\/\u00b5L<\/td>\n<td style=\"width: 20%; height: 24px;\" rowspan=\"2\">\u2265 500 ng<\/td>\n<td style=\"width: 20%; height: 24px;\" rowspan=\"2\">20-50 \u00b5L<\/td>\n<\/tr>\n<tr style=\"height: 24px;\">\n<td style=\"width: 20%; height: 24px;\">RIN \u2265 7<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 10pt;\">* For optimal results, please provide as much material as possible.<\/span><\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                     Data Delivery\n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p>Sequencing data is delivered in FASTQ format. A Globus link will be sent to the email address provided in the sample submission form. The document <a href=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/06\/sequencageNGS_How-To-Log-In-and-Transfer-Files-with-Globus.pdf\" target=\"_blank\" rel=\"noopener\">How to Log In and Transfer Files with Globus<\/a> contains instructions for logging in and transferring files with Globus.<\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                        <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>The Next-Generation Sequencing (NGS) Platform offers high-throughput sequencing services on an Illumina infrastructure, covering various fields such as genomics, transcriptomics, and epigenomics.<\/p>\n","protected":false},"author":10,"featured_media":0,"parent":65954,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"template-platforms-services.blade.php","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-66432","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - 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