{"id":66194,"date":"2024-06-10T15:43:28","date_gmt":"2024-06-10T19:43:28","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/animal-models\/service-de-lanimalerie\/derivation-cryopreservation\/cryopreservation-decongelation\/"},"modified":"2024-07-04T07:58:30","modified_gmt":"2024-07-04T11:58:30","slug":"cryopreservation-decongelation","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/animal-models\/service-de-lanimalerie\/derivation-cryopreservation\/cryopreservation-decongelation\/","title":{"rendered":"Thawing of sperm and embryos"},"content":{"rendered":"<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Luc Filion, TSA<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Assistant head, Derivation and cryopreservation service\n                                    <\/div>\n                                \n                                                                    <div class=\"mb-1\">\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:1 418-525-4444, ext. 46035\">1 418-525-4444, ext. 46035<\/a>\n                                                                            <\/div>\n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:luc.filion@crchudequebec.ulaval.ca\" title=\"luc.filion@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">luc.filion@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                            \n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                    \n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p>This procedure involves thawing the sample of sperm\/embryos and performing implantation into pseudo-pregnant females.<\/p>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n\n\n<section id=\"\" data-block=\"block_f6ee299cf9fab190b968016d01d9eb3f\" class=\"block-accordions align bg-white\">\n    <div class=\"container\">\n        <div class=\"padd\">\n            <div class=\"wrap\">\n                <div class=\"w-full divide-y divide-blue-200 border-blue-200 last:border-b border-t\">\n                                    <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Thawing of Sperm \n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p><span data-contrast=\"none\">For this procedure, you need to provide one to two straws of cryopreserved sperm.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">Please note that fertilization will be done using ova from &#8220;wildtype&#8221; females (genetic background corresponding to that of your lineage). For example, if the sperm from a homozygous male is cryopreserved, the resulting mice from thawing will automatically be heterozygous, and crossings will be necessary if you desire homozygous mice.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">The cost of this procedure guarantees you 10 pups. This guarantee is valid only if the sperm was frozen by the animal facilities service of the CHU de Qu\u00e9bec. Additionally, in certain cases, the cost of the procedure may be subject to change (importation, low fertilization rate, etc.).<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Thawing of Embryos \n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p><span data-contrast=\"none\">For this procedure, you need to provide one to three straws of cryopreserved embryos.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">The cost of this procedure guarantees you one pair (1 male and 1 female). This guarantee is valid only if the embryos were frozen by the animal facilities service of the CHU de Qu\u00e9bec-Universit\u00e9 Laval. Additionally, in certain cases, the cost of the procedure may be subject to change (e.g., importation).<\/span><\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                        <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>This procedure involves thawing the sample of sperm\/embryos and performing implantation into pseudo-pregnant females. <\/p>\n","protected":false},"author":10,"featured_media":0,"parent":66157,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"template-platforms-services.blade.php","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-66194","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Thawing of sperm and embryos - 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