{"id":66175,"date":"2024-06-07T13:11:34","date_gmt":"2024-06-07T17:11:34","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/animal-models\/service-de-lanimalerie\/derivation-cryopreservation\/derivation-sperme-embryons\/"},"modified":"2024-07-03T14:34:47","modified_gmt":"2024-07-03T18:34:47","slug":"derivation-sperme-embryons","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/animal-models\/service-de-lanimalerie\/derivation-cryopreservation\/derivation-sperme-embryons\/","title":{"rendered":"Derivation with cryopreservation of sperm and embryos"},"content":{"rendered":"<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Luc Filion, TSA<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Assistant manager, Derivation and cryopreservation service\n                                    <\/div>\n                                \n                                                                    <div class=\"mb-1\">\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:1 418-525-4444, ext. 46035\">1 418-525-4444, ext. 46035<\/a>\n                                                                            <\/div>\n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:luc.filion@crchudequebec.ulaval.ca\" title=\"luc.filion@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">luc.filion@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                            \n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                    \n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p><span class=\"TextRun SCXW145726843 BCX0\" lang=\"FR-FR\" xml:lang=\"FR-FR\" data-contrast=\"none\"><span class=\"NormalTextRun SpellingErrorV2Themed SCXW145726843 BCX0\">Derivation<\/span><span class=\"NormalTextRun SCXW145726843 BCX0\"> of a mouse <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW145726843 BCX0\">strain<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW145726843 BCX0\">allows<\/span><span class=\"NormalTextRun SCXW145726843 BCX0\"> for <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW145726843 BCX0\">achieving<\/span><span class=\"NormalTextRun SCXW145726843 BCX0\"> a compliant <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW145726843 BCX0\">health<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW145726843 BCX0\">status<\/span><span class=\"NormalTextRun SCXW145726843 BCX0\"> by <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW145726843 BCX0\">removing<\/span><span class=\"NormalTextRun SCXW145726843 BCX0\"> all <\/span><span class=\"NormalTextRun SpellingErrorV2Themed SCXW145726843 BCX0\">potentially<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW145726843 BCX0\">present<\/span> <span class=\"NormalTextRun SpellingErrorV2Themed SCXW145726843 BCX0\">pathogens<\/span><span class=\"NormalTextRun SCXW145726843 BCX0\">.<\/span><\/span><span class=\"EOP SCXW145726843 BCX0\" data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n\n\n<section id=\"\" data-block=\"block_41a74255ff7c34f6100f36152f78ec52\" class=\"block-accordions align bg-white\">\n    <div class=\"container\">\n        <div class=\"padd\">\n            <div class=\"wrap\">\n                <div class=\"w-full divide-y divide-blue-200 border-blue-200 last:border-b border-t\">\n                                    <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Derivation with Sperm Cryopreservation \n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p><span data-contrast=\"none\">This procedure allows for achieving a compliant health status while benefiting from the advantages of cryopreservation. For this procedure, you must provide a minimum of 2 males aged between 3 and 6 months. It is possible to perform the procedure with only one male; however, the yield may be affected.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">The procedure involves harvesting epididymal sperm and freezing them. To ensure the viability of the sperm, validation will be conducted. This involves thawing a straw of cryopreserved sperm, performing in vitro fertilization, and observing the first cell division upon incubation (two-cell stage). The resulting embryos will be implanted into pseudopregnant females. Once the pups are weaned, a health assessment will be conducted before transferring the animals to the researcher. The costs related to this health assessment are not included in the procedure cost.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">The cost of this procedure includes the following:<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"11\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"none\">Guarantee of 18 straws of cryopreserved sperm<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"11\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"auto\">Minimum guarantee of one breeding pair (1 male and 1 female)<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"none\">Please note that fertilization will be performed using eggs from &#8220;wildtype&#8221; females (genetic background corresponding to that of your strain). For example, if the sperm from a homozygous male is cryopreserved, the mice produced after thawing will automatically be heterozygous, and further breeding will be necessary if you wish to obtain homozygous mice.<\/span><\/i><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                            <div\n                    class=\"hover:bg-blue-100 transition-colors duration-300\"\n                    x-data=\"{ expanded: false }\"\n                    x-bind:class=\"{\n                        'bg-blue-100\/100': expanded,\n                        'bg-blue-100\/0': !expanded,\n                    }\"\n                    x-on:click.away=\"expanded = false\"\n                >\n                    <button\n                        class=\"flex flex-row w-full items-center text-left md:px-9 px-5 py-3 md:gap-x-6 gap-x-3 md:min-h-[100px] min-h-[76px]\"\n                        x-on:click=\"expanded = !expanded\"\n                    >\n\n                                                    <div class=\"flex flex-row w-full\">\n                                <h3 class=\"mb-0 text-24 leading-normal font-semibold\">\n                                    Derivation with Embryo Cryopreservation \n                                <\/h3>\n                            <\/div>\n                        \n                        <div class=\"flex ml-auto text-blue-900\">\n                            <i class=\"fa-light md:text-[26px] text-[20px]\"\n                                x-bind:class=\"{\n                                    'fa-plus': !expanded,\n                                    'fa-minus': expanded,\n                                }\"\n                            >\n                            <\/i>\n                        <\/div>\n                    <\/button>\n\n                    <div\n                        class=\"block transition-all ease-in pb-3\"\n                        x-show=\"expanded\"\n                    >\n                                                    <div class=\"md:px-9 px-5\">\n                                <div class=\"inner\">\n                                    <p><span data-contrast=\"none\">This procedure allows for decontamination of a strain while benefiting from the advantages of cryopreservation. For this procedure, you must provide either 2 males to obtain fresh sperm along with 20 to 25 females, or 2 straws of frozen sperm and 20 to 25 females.<\/span><\/p>\n<p><span data-contrast=\"none\">The procedure involves superovulating females to collect eggs and performing in vitro fertilization with fresh or frozen sperm. The resulting embryos will be cryopreserved, and one straw of embryos will be used for implantation into a pseudopregnant female. Once the pups are weaned, a health assessment will be conducted along with the transfer of animals to the researcher. The costs related to this health assessment are not included in the procedure cost.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">The cost of this procedure includes the following:<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"10\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"none\">Guarantee of 10 straws of cryopreserved embryos<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"10\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"auto\">Minimum guarantee of one breeding pair (1 male and 1 female)<\/span><\/li>\n<\/ul>\n\n                                <\/div>\n                            <\/div>\n                                            <\/div>\n                <\/div>\n                        <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Derivation of a mouse strain allows for achieving a compliant health status by removing all potentially present pathogens. <\/p>\n","protected":false},"author":10,"featured_media":0,"parent":66157,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"template-platforms-services.blade.php","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-66175","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - 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