{"id":65772,"date":"2024-05-17T09:58:29","date_gmt":"2024-05-17T13:58:29","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/bioimaging-and-cytometry\/high-throughput-image-analysis\/analyse-de-limmunofluorescence\/"},"modified":"2024-07-09T10:15:45","modified_gmt":"2024-07-09T14:15:45","slug":"immunofluorescence-analysis","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/bioimaging-and-cytometry\/high-throughput-image-analysis\/immunofluorescence-analysis\/","title":{"rendered":"Immunofluorescence Analysis"},"content":{"rendered":"<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    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<\/div>\n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Director<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Denis Soulet, Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Manager<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Marc Bazin, Ph.D.<br \/>\r\n<br \/>\r\nOffice T2-33\n                                    <\/div>\n                                \n                                                                    <div class=\"mb-1\">\n                                                                                    \n                                            <a class=\"phone\" href=\"tel:+1 418-525-4444, ext. 42296 and 42237 \">+1 418-525-4444, ext. 42296 and 42237 <\/a>\n                                                                            <\/div>\n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:analyse.images.haut.debit@crchudequebec.ulaval.ca\" title=\"analyse.images.haut.debit@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">analyse.images.haut.debit@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                            \n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                    \n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p><span data-contrast=\"none\">Fluorescence images are obtained by microscopes geared with a digitizer. Our analysis algorithms take advantage of the separation of signals recorded in each channel of the image (one fluorescence signal per channel). Each channel is cleaned of background noise and the shading effect in the image tiles is normalized. The evaluation of signal intensity becomes more precise. After detecting the objects of interest, they are measured by one or more appropriate mathematical models and possibly filtered according to criteria (size, collocation, form factor, intensity, etc.). In intravital microscopy, the video is stabilized on all channels before analyzing the kinematics (trajectories) of the objects of interest.<\/span><span data-ccp-props=\"{&quot;134245417&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"none\">The overall analysis process performs measurements on an entire batch of images without manual intervention and produces a structured table of results reporting measurements and factors of the experiment usable as is by any statistical analysis software.<\/span><\/p>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Fluorescence images are analyzed by detecting objects of interest after subtracting background noise, correcting the tile effect, image stabilization (video).<\/p>\n","protected":false},"author":37,"featured_media":0,"parent":65685,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"template-platforms-services.blade.php","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-65772","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - 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