{"id":64343,"date":"2024-05-08T08:57:42","date_gmt":"2024-05-08T12:57:42","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/bioimaging-and-cytometry\/cellular-imaging-unit\/microscopie-par-reflexion-totale-interne-a-fluorescence\/"},"modified":"2024-06-28T08:52:22","modified_gmt":"2024-06-28T12:52:22","slug":"total-internal-reflection-fluorescence-microscopy","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/bioimaging-and-cytometry\/cellular-imaging-unit\/total-internal-reflection-fluorescence-microscopy\/","title":{"rendered":"Total Internal Reflection Fluorescence Microscopy"},"content":{"rendered":"<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Technical Manager<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Carl St-Pierre\n                                    <\/div>\n                                \n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:uic@crchudequebec.ulaval.ca\" title=\"uic@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">uic@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Chair of the Cellular Imaging Unit Users Committee<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Jos\u00e9e N. Lavoie, Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Co-chair of the Cellular Imaging Unit Users Committee<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Marc-\u00c9tienne Huot, Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                            \n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                                            <figure class=\"md:mb-12 mb-6\">\n                            <img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"887\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_TIRF.jpg\" class=\"rounded-3xl\" alt=\"\" srcset=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_TIRF.jpg 1920w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_TIRF-300x139.jpg 300w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_TIRF-1024x473.jpg 1024w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_TIRF-768x355.jpg 768w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_TIRF-1536x710.jpg 1536w\" sizes=\"auto, (max-width: 1920px) 100vw, 1920px\" \/>\n\n                                                    <\/figure>\n                    \n                                            <div class=\"highlight-text md:mb-12 mb-7\">\n                            <p>\n                                Total Internal Reflection Fluorescence Microscopy: Nikon H-TRIF\n                            <\/p>\n                        <\/div>\n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p><strong>Alias:\u00a0<\/strong><\/p>\n<p>TIRF<\/p>\n<p><strong>Primary Application:\u00a0<\/strong><\/p>\n<p>Acquisition of phenomena at the cell-coverslip interface, with excitation limited to a domain of ~100 nm in z.<\/p>\n<p><strong><u>Specifications<\/u><\/strong><\/p>\n<p><strong>Microscope Stand:\u00a0<\/strong><\/p>\n<ul>\n<li>Inverted microscope Nikon Ti-E<\/li>\n<\/ul>\n<p><strong>Cameras:\u00a0<\/strong><\/p>\n<ul>\n<li>Hamamatsu Orca ER-C4742-0812AG CCD; 1344 (H) x 1024 (V); 6.45 \u00b5m\/pixel<\/li>\n<li>Andor Xion DU-897E EMCCD; 512 x 512; 16 \u00b5m\/pixel<\/li>\n<\/ul>\n<p><strong>Fluorescence Acquisition Light Source:\u00a0<\/strong><\/p>\n<ul>\n<li>TIRF acquisition: Nikon LUN-4 laser diodes: 405 nm, 488 nm, 561 nm, 640 nm<\/li>\n<li>Epifluorescence: Lumencor Sola SE LED (UV at 365 nm)<\/li>\n<\/ul>\n<p><strong>Flourescence Filter Sets:\u00a0<\/strong><\/p>\n<ul>\n<li>DAPI :\u00a0 Chroma 49028-ET; Ex 395\/25 nm, Em 460\/50nm, DM 425nm<\/li>\n<li>GFP : Chroma TRF49904-ET; Ex 488\/10 nm, Em 525\/50nm, DM 500 nm LP<\/li>\n<li>RFP : Chroma TRF49909-ET; Ex 561\/10 nm; Em 600\/50 nm; DM 575 nm LP<\/li>\n<li>GFP-RFP : Chroma TRF59904-ET: Dual Ex 488\/10 nm &amp; 561\/10 nm; Dual EM 525\/50nm &amp; 600\/50 nm<\/li>\n<li>CY5 : Chroma 41008: Ex 620\/60 nm; Em 700\/75 nm; DM 660 nm LP<\/li>\n<\/ul>\n<p><strong>Objectives and calibrations :<\/strong><\/p>\n<table>\n<tbody>\n<tr>\n<td>Objectives<\/td>\n<td>Calibration<\/td>\n<td>Andor Xion<\/td>\n<\/tr>\n<tr>\n<td><\/td>\n<td>\u00b5m\/pixel @ bin 1&#215;1<\/td>\n<td>\u00b5m\/pixel @ bin 1&#215;1<\/td>\n<\/tr>\n<tr>\n<td>Nikon Plan Fluor 4x 0.13 NA<\/td>\n<td>1,613<\/td>\n<td>4,000<\/td>\n<\/tr>\n<tr>\n<td>Nikon Plan Apo 10x 0.45 NA DIC N2<\/td>\n<td>0,641<\/td>\n<td>1,600<\/td>\n<\/tr>\n<tr>\n<td>Nikon Plan Fluor ELWD 20x 0.45 NA Ph1<\/td>\n<td>0,322<\/td>\n<td>0,800<\/td>\n<\/tr>\n<tr>\n<td>Nikon Plan Apo VC 20x 0.75 NA DIC N2<\/td>\n<td>0,322<\/td>\n<td>0,800<\/td>\n<\/tr>\n<tr>\n<td>Nikon Plan Apo Lambda 40x 0,95 NA DIC N2<\/td>\n<td>0,161<\/td>\n<td>0,400<\/td>\n<\/tr>\n<tr>\n<td>Nikon Apo TIRF 60x 1.49 NA oil TIRF<\/td>\n<td>0,108<\/td>\n<td>0,267<\/td>\n<\/tr>\n<tr>\n<td>Nikon Plan Apo VC 60x 1.30 NA water<\/td>\n<td>0,108<\/td>\n<td>0,267<\/td>\n<\/tr>\n<tr>\n<td>Nikon Plan Apo 100x 1.40 NA oil DIC N2<\/td>\n<td>0,065<\/td>\n<td>0,160<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Other Nikon objectives available upon request.<\/p>\n<p><strong>Other components:<\/strong><\/p>\n<ul>\n<li>Perfect Focus System (PFS: automated focus maintenance)<\/li>\n<li>Mightex Polygon 400 Photostimulator<\/li>\n<li>MCL 100 \u00b5m Piezo-Z<\/li>\n<li>3D Tracking and Measurement analysis module on NIS Elements<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Inverted epifluorescence microscope with TIRF module and Perfect Focus System. Environmental chamber at 37\u00b0C and 5% CO2.<\/p>\n","protected":false},"author":37,"featured_media":0,"parent":64165,"menu_order":11,"comment_status":"closed","ping_status":"closed","template":"template-platforms-services.blade.php","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-64343","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Total Internal Reflection Fluorescence Microscopy - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/bioimaging-and-cytometry\/cellular-imaging-unit\/total-internal-reflection-fluorescence-microscopy\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Total Internal Reflection Fluorescence Microscopy - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval\" \/>\n<meta property=\"og:description\" content=\"Inverted epifluorescence microscope with TIRF module and Perfect Focus System. 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