{"id":64195,"date":"2024-05-06T15:18:28","date_gmt":"2024-05-06T19:18:28","guid":{"rendered":"https:\/\/www.crchudequebec.ulaval.ca\/platforms-and-services\/bioimaging-and-cytometry\/cellular-imaging-unit\/microscope-confocal-a-balayage-laser-zeiss-lsm-900-airyscan-ii\/"},"modified":"2024-06-28T08:35:55","modified_gmt":"2024-06-28T12:35:55","slug":"laser-scanning-confocal-microscope-zeiss-lsm-900-airyscan-ii","status":"publish","type":"page","link":"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/bioimaging-and-cytometry\/cellular-imaging-unit\/laser-scanning-confocal-microscope-zeiss-lsm-900-airyscan-ii\/","title":{"rendered":"Laser Scanning Confocal Microscope: Zeiss LSM 900 AiryScan II"},"content":{"rendered":"\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<section class=\"bg-white align wp-block-acf-content-with-left-sidebar\">\n    <div class=\"container\">\n    <div class=\"padd padd--bottom\">\n        <div class=\"wrap\">\n            <div class=\"flex md:flex-row flex-col md:gap-x-[8.3%] gap-x-3 md:gap-y-0 gap-y-8\">\n                <div class=\"sidebar md:w-1\/4 w-full flex flex-col gap-y-8\">\n                    \n                    \n                                                                        <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Technical Manager<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Carl St-Pierre\n                                    <\/div>\n                                \n                                \n                                                                    <ul class=\"emails !mb-3\">\n                                                                                    <li class=\"overflow-hidden\">\n                                                <a class=\"group flex items-center\" href=\"mailto:uic@crchudequebec.ulaval.ca\" title=\"uic@crchudequebec.ulaval.ca\">\n                                                    <i class=\"fa-light fa-envelope shrink-0 group-hover:text-blue-500 duration-300\"><\/i>\n                                                    <span class=\"text-16 whitespace-nowrap overflow-hidden text-ellipsis group-hover:text-blue-500 duration-300\">uic@crchudequebec.ulaval.ca<\/span>\n                                                <\/a>\n                                            <\/li>\n                                                                            <\/ul>\n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Chair of the Cellular Imaging Unit Users Committee<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Jos\u00e9e N. Lavoie, Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                                    <div>\n                                                                    <h3 class=\"mb-0 text-16 font-bold\">Co-chair of the Cellular Imaging Unit Users Committee<\/h3>\n                                \n                                                                    <div class=\"mb-1\">\n                                        Marc-\u00c9tienne Huot, Ph.D.\n                                    <\/div>\n                                \n                                \n                                \n                                                            <\/div>\n                                            \n                    \n                    \n                    \n                                    <\/div>\n                <div class=\"main-content md:w-7\/12 w-full\">\n                                            <figure class=\"md:mb-12 mb-6\">\n                            <img loading=\"lazy\" decoding=\"async\" width=\"1920\" height=\"887\" src=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_LSM900.jpg\" class=\"rounded-3xl\" alt=\"\" srcset=\"https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_LSM900.jpg 1920w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_LSM900-300x139.jpg 300w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_LSM900-1024x473.jpg 1024w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_LSM900-768x355.jpg 768w, https:\/\/www.crchudequebec.ulaval.ca\/wp-content\/uploads\/2024\/04\/Plateforme_imageriecell_LSM900-1536x710.jpg 1536w\" sizes=\"auto, (max-width: 1920px) 100vw, 1920px\" \/>\n\n                                                    <\/figure>\n                    \n                    \n                                            <div class=\"content\">\n                            <div class=\"inner\">\n                                <p><strong>Alias:\u00a0<\/strong><\/p>\n<p><span data-contrast=\"auto\">LSM 900<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><strong>Key Applications:\u00a0<\/strong><\/p>\n<p><span data-contrast=\"auto\">High-resolution imaging (~140 nm in x,y) with AiryScan II module. Z-stack acquisition. FRAP (Fluorescence Recovery After Photobleaching).<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span style=\"text-decoration: underline;\"><strong>Specifications<\/strong><\/span><\/p>\n<p><strong>Microscope Stand:\u00a0<\/strong><\/p>\n<ul>\n<li><span data-contrast=\"auto\">Inverted microscope Zeiss Axio Observer Z.1<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><strong>Confocal Head:\u00a0<\/strong><\/p>\n<ul>\n<li><span data-contrast=\"auto\">LSM 900 with 2 PMT GaAsP detectors and 1 AiryScan II GaAsP detector<\/span><\/li>\n<\/ul>\n<p><strong>Objectives:\u00a0<\/strong><\/p>\n<ul>\n<li><span data-contrast=\"auto\">Plan-Apochromat 20x 0.80 NA<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li><span data-contrast=\"auto\">Plan-Apochromat 63x OIL 1.40 NA<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li><span data-contrast=\"auto\">C-Apochromat 40x 1.1 NA Water<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li><span data-contrast=\"auto\">Plan-NeoFluar 40x OIL 1.30 NA<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li><span data-contrast=\"auto\">LD Plan-NeoFluar 40x 0.60 NA KORR<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><strong>Laser Sources (acquisition):\u00a0<\/strong><\/p>\n<ul>\n<li data-leveltext=\"\uf02d\" data-font=\"Symbol\" data-listid=\"42\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;center&quot;,&quot;469777804&quot;:&quot;\uf02d&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">Laser diodes: 405 nm, 488 nm, 561 nm, 639 nm<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><strong>Filters (acquisition):\u00a0<\/strong><\/p>\n<ul>\n<li data-leveltext=\"\uf02d\" data-font=\"Symbol\" data-listid=\"41\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;center&quot;,&quot;469777804&quot;:&quot;\uf02d&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">Variable spectral detection (VSD)<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><strong>Fluorescence Illumination Source:\u00a0<\/strong><\/p>\n<ul>\n<li data-leveltext=\"\uf02d\" data-font=\"Symbol\" data-listid=\"40\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;center&quot;,&quot;469777804&quot;:&quot;\uf02d&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">Exfo X-Cite metal halide<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><strong>Fluorescence Observation Filters:\u00a0<\/strong><\/p>\n<ul>\n<li data-leveltext=\"\uf02d\" data-font=\"Symbol\" data-listid=\"39\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;center&quot;,&quot;469777804&quot;:&quot;\uf02d&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">DAPI Zeiss filter set 49: EX 365\/30 nm &#8212; EM 445\/50 nm<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li data-leveltext=\"\uf02d\" data-font=\"Symbol\" data-listid=\"39\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;center&quot;,&quot;469777804&quot;:&quot;\uf02d&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"auto\">GFP Zeiss filter set 10: EX 470\/40 nm &#8212; EM 540\/50 nm<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li data-leveltext=\"\uf02d\" data-font=\"Symbol\" data-listid=\"39\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;center&quot;,&quot;469777804&quot;:&quot;\uf02d&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"3\" data-aria-level=\"1\"><span data-contrast=\"auto\">RFP Zeiss filter set 20: EX 549\/12 nm &#8212; EM 608\/65 nm<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><strong>Software:\u00a0<\/strong><\/p>\n<ul>\n<li data-leveltext=\"\uf02d\" data-font=\"Symbol\" data-listid=\"43\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;center&quot;,&quot;469777804&quot;:&quot;\uf02d&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">Zeiss Zen Blue 3.3<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><strong>Other Components:\u00a0<\/strong><\/p>\n<ul>\n<li data-leveltext=\"\uf02d\" data-font=\"Symbol\" data-listid=\"43\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;center&quot;,&quot;469777804&quot;:&quot;\uf02d&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"auto\">Environmental chamber at 37\u00b0C and 5% CO2 for 35 mm plates and petri dishes<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf02d\" data-font=\"Symbol\" data-listid=\"43\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;center&quot;,&quot;469777804&quot;:&quot;\uf02d&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"auto\">Motorized stage<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf02d\" data-font=\"Symbol\" data-listid=\"43\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;center&quot;,&quot;469777804&quot;:&quot;\uf02d&quot;,&quot;469777815&quot;:&quot;hybridMultilevel&quot;}\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"auto\">Transmitted light detector (T-PMT)<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n\n                            <\/div>\n                        <\/div>\n                    \n                    \n                    \n                    \n                                    <\/div>\n            <\/div>\n        <\/div>\n    <\/div>\n<\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Inverted Laser Scanning Confocal Microscope equipped with spectral detectors PMT GaAsP and AiryScan II. <\/p>\n","protected":false},"author":37,"featured_media":0,"parent":64165,"menu_order":15,"comment_status":"closed","ping_status":"closed","template":"template-platforms-services.blade.php","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-64195","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Laser Scanning Confocal Microscope: Zeiss LSM 900 AiryScan II - Centre de recherche du CHU de Qu\u00e9bec-Universit\u00e9 Laval<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.crchudequebec.ulaval.ca\/en\/platforms-and-services\/bioimaging-and-cytometry\/cellular-imaging-unit\/laser-scanning-confocal-microscope-zeiss-lsm-900-airyscan-ii\/\" \/>\n<meta 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