Our platform is equipped with various state-of-the-art technologies to meet with the proteomic needs of most life scientists. Whether your objective is to achieve a high sensitivity or a high precision in your proteomic experiment, we have a personalized, cutting-edge solution for you.
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Orbitrap Exploris 480 (Thermo) / Evosep One (Evosep Biosystems)
The Orbitrap Exploris 480 is the latest generation of Orbitrap instrument. This mass spectrometer provides high resolution spectra (up to 480 000 K) allowing high accuracy measurements (<2ppm). It is particularly suitable for the analysis of high complexity protein mixtures through its very sensitive Data Independent Acquisition (DIA) mode. This mass spectrometer is also equipped with a FAIMS Pro ion mobility device allowing noise reduction in mass spectra.
Our Orbitrap Exploris 480 is interfaced with an Evosep One liquid chromatography system which is ideal for high throughput analyses. Indeed, the Evosep One allowed the analysis of 30 to 300 samples per day with high robustness and high reproducibility. Moreover, it uses disposable pre-columns avoiding cross-contamination from sample to sample.
Together, the Orbitrap Exploris 480 and the Evosep enable the identification and accurate quantification of thousands of proteins (depending on the type of sample) in studies involving hundreds of biological samples.
Orbitrap Fusion (Thermo Fisher)
The Orbitrap Fusion Tribrid is a mass spectrometer composed of three types of analyzers: a quadrupole (for an efficient isolation of ions), a linear trap (for a fast detection of fragmentation spectra), and an orbitrap (for a very accurate mass measurement due to its very high resolution up to 450K).
In addition to the classical CID fragmentation, this mass spectrometer is able to perform other types of peptide fragmentation through its HCD collision cell (very useful for iTRAQ or TMT quantification) and its ETD source which is more efficient for post-translational modifications identification like phosphorylation, glycosylation, etc.
This instrument is online with a Thermo nanoRSLC U3000 system (ultra high pressure liquid chromatography up to 800 bars) which make it very powerful for the analysis of very complex protein mixtures (identification of several thousands of proteins in one run). Its sensitivity and its versatility make it the instrument of choice for challenging samples (search for PTMs, high complexity,…)
QTRAP 6500 (SCIEX)
The QTRAP 6500 is a quadrupole ion trap mass spectrometer is interfaced with a Thermo U3000 RSLC nano system (ultra high pressure liquid chromatography up to 800 bars) and a nanospray source.
This LC/MS/MS system is ultra-sensitive for targeted protein or peptide biomarker relative or absolute quantification. It is used for MRM/SRM (Multiple/Single Reaction Monitoring) assays. With these techniques, after an optimisation of the conditions specific to the targeted proteins, many targeted proteins can be quantified in a single injection. These techniques also allow for the comparison of targeted protein expression levels across multiple samples.
TSQ Altis and Vanquish Neo (Thermo Fisher)
Targeted MS, and in particular selected reaction monitoring (SRM), is the quantitative analysis method of choice on the TSQ Altis Triple Quadrupole Mass Spectrometer, enabling monitoring of up to 250 peptides per second while using 0.2-Dalton ion selection. This dramatically reduces chemical interferences while maintaining high transmission efficiency.
This instrument is coupled with a Thermo Vanquish Neo, the latest generation of liquid chromatography systems enabling wide variety of analytical flowrates (from nano to micro) for the best compromise between throughput and sensitivity.