Dr. Roxane Pouliot is a regular researcher at the CHU Research Centre of Quebec-Laval University, and Professor at the Faculty of Pharmacy of Laval University. As a regular member, she is also part of the Centre for Advanced Materials Research (CAMaR), as well as the Centre Québécois on Functional Materials (CQMF). Her research interests focus on a better understanding of the structures and functions of skin tissue through advanced models of tissue reconstruction. Her team recently developed an immunocompetent psoriatic skin replacement model reconstructed from human cells, a major breakthrough in this area.

Dr. Pouliot is the recipient of Laval University’s 2016 Graduate Teaching and Coaching Award.

Understanding the complexity of structures and functions of skin tissue

In vitro skin diffusion tests are one of the first steps in the development of a transdermal drug. To this end, a robust model of cutaneous biomaterial is necessary for the precise study of the diffusion mechanisms of therapeutic molecules. Advanced lipidomic characterization of tissue-engineered samples is essential for understanding the skin lipid domain, which is complex and poorly reproduced in vitro. However, the biomimetic quality currently developed within our research team allows us to consider improving the models to obtain the qualities found within a native skin, whether healthy or pathological.

Modelling psoriasis and developing new pharmacological strategies

Cutaneous diseases such as psoriasis are complex diseases whose development and persistence involve several cellular processes and cell types. In recent years, our research team has developed a world-unique model of reconstructed psoriatic skin. Through tissue engineering, it becomes possible to reconstruct, according to the self-assembly method, a completely autologous pathological skin, that is to say without exogenous material, and composed of both a dermis and an epidermis. This model of pathological skin provides us with the opportunity to study separately the players involved in the development of psoriasis, and therefore becomes a powerful tool in the mechanistic analysis of this complex pathology. Distinctly, the latter is three-dimensional, designed from psoriatic human cells, in addition to developing in a highly physiological environment.

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Jean J, Pouliot R

In vivo and in vitro models of psoriasis

Book Chapter

Eberli Daniel (Ed.): Tissue Engineering, pp. 359-382, 2010.

Larouche D, Jean J, Berthod F, Germain L, Pouliot R

Markers for an in vitro skin substitute

Book Chapter

Maguire T, Novik E (Ed.): Alternative technologies to animal testing, pp. 183-203, Boston, MA, Artech House, 2010.

Pouliot R

In vitro: un nouveau modèle cutané pour des traitements plus efficaces du psoriasis

Journal Article

44 (22), 2009.

Jean J, Lapointe M, Soucy J, Pouliot R

Development of an in vitro psoriatic skin model by tissue engineering

Journal Article

J Dermatol Sci, 53 (1), 2009.

Abstract | Links:

Auger FA, Berthod F, Moulin V, Pouliot R, Germain L

Tissue-engineered skin substitutes: from in vitro constructs to in vivo applications

Journal Article

Biotechnol Appl Biochem, 39 (Pt 3), 2004.

Abstract | Links:

Pouliot R, Larouche D, Auger FA, Juhasz J, Xu W, Li H, Germain L

Reconstructed human skin produced in vitro and grafted on athymic mice

Journal Article

Transplantation, 73 (11), 2002.

Abstract | Links:

Auger FA, Pouliot R, Tremblay N, Guignard R, Noel P, Juhasz J, Germain L, Goulet F

Multistep production of bioengineered skin substitutes: sequential modulation of culture conditions

Journal Article

In Vitro Cell Dev Biol Anim, 36 (2), 2000.

Abstract | Links:

Michel M, L'Heureux N, Pouliot R, Xu W, Auger FA, Germain L

Characterization of a new tissue-engineered human skin equivalent with hair

Journal Article

In Vitro Cell Dev Biol Anim, 35 (6), 1999.

Abstract | Links:

Pouliot R, Germain L, Auger FA, Tremblay N, Juhasz J

Physical characterization of the stratum corneum of an in vitro human skin equivalent produced by tissue engineering and its comparison with normal human skin by ATR-FTIR spectroscopy and thermal analysis (DSC)

Journal Article

Biochim Biophys Acta, 1439 (3), 1999.

Abstract | Links:

Pouliot R, Robert M, Germain L, Noel-Hudson M, Lindenbaum A, Juhasz J, Auger FA, Wepierre J

Influence of endothelial cells on structure, biochemistry and functionality of epidermis reconstructed on synthetic porous membrane

Journal Article

Skin Pharmacol Appl Skin Physiol, 11 (4-5), 1998.

Abstract | Links:

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Active projects

  • A human tissue-engineered three-layer cornea (hTEC) supplemented with macrophages as a biomaterial for in vitro studies, from 2024-04-01 to 2029-03-31
  • Breaking barriers in drug delivery via using viscoplastic fluids for precise control of jet evolution and penetration, from 2024-03-31 to 2026-03-30
  • Centre Québécois sur les Matériaux Fonctionnels, from 2024-04-01 to 2030-03-31
  • Comprendre la complexité du domaine lipidique cutané, from 2019-04-01 to 2027-03-31
  • Nouvelles options thérapeutiques pour les patients atteints de psoriasis, from 2019-10-01 to 2024-09-30

Recently finished projects

  • Centre Québécois sur les Matériaux Fonctionnels (CQMF), from 2017-04-01 to 2024-03-31
  • Étude de l'impact de l'innervation cutanée dans le Psoriasis, from 2018-04-01 to 2023-03-31
Data provided by the Université Laval research projects registery