Trained as a biochemist (UQAM, 1989), and as a molecular and cellular biologist (U. of Montreal 1996 and Karolinska Institutet, Stockholm, Sweden, 2001), Dr. Patrick Provost is a Full Professor in the Department of Microbiology, Infectious diseases and Immunology at the Faculty of Medicine of Université Laval, and a Researcher at the CHU de Québec Research Center/CHUL Pavilion since 2001. To date, the turning point in his career has been his co-discovery, in 2002, of the ribonuclease Dicer in humans. This enzyme catalyzes the formation of microRNAs (19 to 24 nucleotides long), which are now recognized as key regulators of ~60% of the genes in humans.
His current research aims to improve our understanding of the molecular mechanisms underlying the biogenesis, function and transfer of small non-coding RNAs (e. g. microRNAs) between cells and species through extracellular vesicles (EVs; 0.1-1 µm in diameter) in the context of health, nutrition and disease.
His work has been regularly covered by the media, given their importance and translational nature.
Content and therapeutic applications of extracellular vesicles (EVs) in milk
EVs are released from a wide variety of cells in body fluids, including milk. The analysis of cow’s milk intended for human consumption revealed that it contains a new type of EVs, which carries most of the microRNAs (Benmoussa et al., J. Extracell. Vesicles 2017; PMID: 29904572) and is able to protect their microRNAs from degradation during simulated digestion (i. e. under the biophysical, chemical and biochemical conditions prevailing in the human gastrointestinal tract) (Benmoussa et al., J. Nutr. 2016; PMID: 27708120).
We also determined that these EVs isolated from milk were enriched with anti-inflammatory proteins, such as epidermal growth factor 8 (MFG-E8) (Benmoussa et al., J. Proteomics 2019; PMID: 30153512).
These results prompted us to explore the therapeutic potential of milk EVs under inflammatory conditions affecting the gastrointestinal tract.
A unique model to evaluate the oral transfer of dietary microRNAs
It has long been believed that the genetic material (e. g. microRNAs) in food is rapidly degraded during digestion. However, we have demonstrated that microRNAs in cow’s milk intended for human consumption are bioaccessible, partly due to the protective effects of EVs. The ability of milk EVs to protect their labile cargo of bioactive molecules during digestion opens the way for the possible oral transfer of dietary microRNAs. This possibility will be verified through a unique model, which, combined with advanced techniques and approaches, will allow us to confirm (or invalidate) the oral transfer of milk microRNAs.
A new class of small non-coding RNAs
At the heart of the genetic information that flows through our cells (DNA is transcribed into RNA, which is translated into proteins), small species of RNA that do not code for proteins, such as microRNAs (19 to 24 nucleotides (nt) long), are now recognized as key regulators of gene expression.
Contrary to the current scientific dogma, according to which there is no endogenous RNA shorter than 16 nt that can be biologically relevant, we have serendipitously discovered a 12-nt RNA species capable of competing with microRNA regulation of mRNA translation into proteins in human cells (Plante et al., Front. Genet. 2012; PMID: 22675332).
More extensive and in-depth analyses have enabled us to identify a new class of small non-coding RNAs. Our current research aims to elucidate the biogenesis pathway and effector ribonucleoprotein complexes involved in the role and function of these small non-coding RNAs in human and mouse cells.
An Expanded Landscape of Unusually Short RNAs in 11 Samples from Six Eukaryotic OrganismsJournal Article
Noncoding RNA, 8 (3), 2022.
Ebola Virus Encodes Two microRNAs in Huh7-Infected CellsJournal Article
Int J Mol Sci, 23 (9), 2022.
A New Specific and Sensitive RT-qPCR Method Based on Splinted 5' Ligation for the Quantitative Detection of RNA Species Shorter than microRNAsJournal Article
Noncoding RNA, 7 (3), 2021.
Identification of Abundant and Functional dodecaRNAs (doRNAs) Derived from Ribosomal RNAJournal Article
Int J Mol Sci, 22 (18), 2021.
Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIVJournal Article
Pathog Immun, 6 (1), 2021.
Velocity Gradient Separation Reveals a New Extracellular Vesicle Population Enriched in miR-155 and Mitochondrial DNAJournal Article
Pathogens, 10 (5), 2021.
Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutritionJournal Article
Sci Rep, 11 (1), 2021.
Altered microRNA Transcriptome in Cultured Human Liver Cells upon Infection with Ebola VirusJournal Article
Int J Mol Sci, 22 (7), 2021.
Isolating Multiple Extracellular Vesicles Subsets, Including Exosomes and Membrane Vesicles, from Bovine Milk Using Sodium Citrate and Differential UltracentrifugationJournal Article
Bio Protoc, 10 (11), 2020.
Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived MicroparticlesJournal Article
Front Cardiovasc Med, 7 , 2020.
- A novel class of small non-coding RNAs, from 2019-04-01 to 2024-03-31
- A unique model to assess the oral transfer of dietary microRNAs, from 2019-10-01 to 2024-09-30