Patrick J. Rochette is a regular researcher at the Research Centre of the CHU of Quebec – Laval University, in the area of ​​regenerative medicine, of which he is the assistant director. He is also Associate Professor and Assistant Director of Basic Research in the Department of Ophthalmology and ENT – head and neck surgery of the Faculty of Medicine at Laval University. He completed his graduate studies (MSc / PhD) in the laboratory of Dr. Regen Drouin (U. Laval), where he studied the induction and repair of UV-induced damage. He pursued his training with 2 postdoctoral fellowships at Yale University, including one in the laboratory of Dr. Douglas Brash, a pioneer in the study of UV-induced mutations in cutaneous cancer. His second fellowship took place in Dr. Yilun Liu ‘s laboratory, where he worked on the RecQ4 helicase in the initiation of DNA replication. He joined Laval University in 2009 and started a laboratory where he specialized in photobiology and, more precisely, on the consequences of cell exposure to low-wavelength light rays (ultraviolet and blue). In humans, only the skin and eyes are exposed to these rays. Thus, Dr. Rochette’s laboratory is interested in two fields of study, ocular photobiology and cutaneous photobiology.

Cutaneous photobiology

At the cutaneous level, skin cells are used as a model to study the mechanisms of protection against the genotoxicity of sunlight. These mechanisms include the induction of mutagenic damage, its repair, and induced cell death as a protective mechanism. In this part of the laboratory, the projects pursued are, among others, the following:

– Influence of the dermis on the efficacy of repair techniques of UV-induced mutagenic damage to the epidermis;

– Effect of chronic UVB irradiation on the cellular genotoxic stress response;

– Necroptosis as a new UV-induced, programmed cell death pathway;

– Involvement of UVA, alone or in synergy with polycyclic aromatic hydrocarbons (PAH) contained in cigarette smoke in cutaneous photoaging.

 Ocular photobiology

At the ocular level, Dr. Rochette’s research is mostly applied. It aims to understand the involvement of UV and blue light in various ocular pathologies. As UV light does not reach the adult retina, blue light is the most energetic light it is exposed to. In this part of the laboratory, the projects pursued are, among others, the following:

– UVA toxicity in the cornea, involvement of UV-Induced oxidation;

– Involvement of mitochondrial dysfunction in Fuchs corneal endothelial dystrophy;

– Involvement of blue light, alone or in synergy with lipofuscin and / or PAHs in age-related macular degeneration (AMD).

Hôpital Saint-Sacrement
1050, chemin Sainte-Foy
CUO-Recherche
Québec, Québec
Canada G1S 4L8
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Gary AS, Amouret S, Montoni A, Rochette PJ

MLKL, a new actor of UVB-induced apoptosis in human diploid dermal fibroblasts

Journal Article

Cell Death Discov, 10 (1), 2024.

Abstract | Links:

Tchatchouang A, Brunette I, Rochette PJ, Proulx S

Expression and Impact of Fibronectin, Tenascin-C, Osteopontin, and Type XIV Collagen in Fuchs Endothelial Corneal Dystrophy

Journal Article

Invest Ophthalmol Vis Sci, 65 (4), 2024.

Abstract | Links:

Larnac E, Montoni A, Haydont V, Marrot L, Rochette PJ

Lipid Peroxidation as the Mechanism Underlying Polycyclic Aromatic Hydrocarbons and Sunlight Synergistic Toxicity in Dermal Fibroblasts

Journal Article

Int J Mol Sci, 25 (3), 2024.

Abstract | Links:

Görlitz M, Justen L, Rochette PJ, Buonanno M, Welch D, Kleiman NJ, Eadie E, Kaidzu S, Bradshaw WJ, Javorsky E, Cridland N, Galor A, Guttmann M, Meinke MC, Schleusener J, Jensen P, Söderberg P, Yamano N, Nishigori C, O'Mahoney P, Manstein D, Croft R, Cole C, de Gruijl FR, Forbes PD, Trokel S, Marshall J, Brenner DJ, Sliney D, Esvelt K

Assessing the safety of new germicidal far-UVC technologies

Journal Article

Photochem Photobiol, 2023.

Abstract | Links:

Grenier A, Morissette MC, Rochette PJ, Pouliot R

Toxic Interaction Between Solar Radiation and Cigarette Smoke on Primary Human Keratinocytes

Journal Article

Photochem Photobiol, 99 (5), 2023.

Abstract | Links:

Méthot S, Proulx S, Brunette I, Rochette PJ

Rescuing cellular function in Fuchs endothelial corneal dystrophy by healthy exogenous mitochondrial internalization

Journal Article

Sci Rep, 13 (1), 2023.

Abstract | Links:

Dorr MM, Rochette PJ

Reduction of DNA damage repair efficiency and accumulation of residual damage following chronic UVB-irradiation of HaCaT cells

Journal Article

PLoS One, 18 (4), 2023.

Abstract | Links:

Zinflou C, Rochette PJ

Indenopyrene and Blue-Light Co-Exposure Impairs the Tightly Controlled Activation of Xenobiotic Metabolism in Retinal Pigment Epithelial Cells: A Mechanism for Synergistic Toxicity

Journal Article

Int J Mol Sci, 24 (24), 2023.

Abstract | Links:

Méthot S, Proulx S, Brunette I, Rochette PJ

The Presence of Guttae in Fuchs Endothelial Corneal Dystrophy Explants Correlates With Cellular Markers of Disease Progression

Journal Article

Invest Ophthalmol Vis Sci, 64 (5), 2023.

Abstract | Links:

Grenier A, Morissette MC, Rochette PJ, Pouliot R

The combination of cigarette smoke and solar rays causes effects similar to skin aging in a bilayer skin model

Journal Article

Sci Rep, 13 (1), 2023.

Abstract | Links:

53 entries « 1 of 6 »
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Active projects

  • Fuchs endothelial corneal dystrophy and corneal transplantation: The traditional paradigm revisited, from 2019-10-01 to 2024-09-30
  • Journée annuelle de la recherche en ophtalmologie , from 2019-04-23 to 2025-03-31
  • Toxicité de la lumière et dysfonctionnement mitochondrial dans l’oeil humain, from 2021-07-01 to 2024-06-30
  • Toxicité des rayons ultraviolets sous l’angle de la mitochondrie, from 2023-04-01 to 2028-03-31
Data provided by the Université Laval research projects registery