Pr. Fradette has been a regular researcher at the CRCHU de Québec-Laval University since 2005, and a full professor of the Department of Surgery of the Laval University School of Medicine. After a bachelor’s degree in biochemistry (1991-1994, U Laval) and a PhD in cellular and molecular biology focused on the study of skin epithelial stem cells (1994-1999, U Laval, with L. Germain, LOEX), she acquired a specialization in gene therapy during her postdoctoral fellowship at the University of Pittsburgh (1999-2004, with J. Glorioso III, U Pittsburgh, PA, USA). Her current research program is based on the use of human adipose-derived stromal/stem cells in regenerative medicine. Since September of 2015, she has been the director of the ThéCell network, the province of Quebec network for cell and tissue therapy supported by the FRQS.
Development of human reconstructed tissues as 3D discovery models and for clinical applications
Pr. Fradette’s current research program is based on the use of a new and abundant source of multipotential cells: the adipose-derived stromal/stem cells (ASCs). Using tissue-engineering techniques, her team uses ASCs to recreate human tissue which is needed in reconstructive surgery, such as skin, adipose, and bone-like tissue. For example, human adipose substitutes recreating the 3D tissular context in vitro are useful discovery tools for pharmaco-toxicological studies on the metabolic regulation of human adipocytes. Moreover, adipose tissues are reconstructed with the important goal of being used in the clinic as soft-tissue fillers to repair tissue loss, in cases of deep burns or tumor removal (including mastectomy). The resulting tissues are all human, and devoid of exogenous biomaterials.
Her research interests include a better understanding of the cellular interactions between adipocytes and endothelial cells, the effects of inflammatory conditions on blood capillaries and adipocyte metabolism, the impact of human ECM components on the adipogenic differentiation of ASCs, and how MMPs can influence tissue remodeling, under normal and inflammatory conditions. Current studies performed in her laboratory aim at promoting the vascularisation of thick reconstructed tissues produced under xenogenic-free culture conditions for clinical applications. Ongoing preclinical studies assess the efficacy of tissue-engineered biological dressings to accelerate the healing of skin wounds.
In collaboration with D.r M. Fortin and Dr. FA. Auger, her team also produces bone-like substitutes from ASCs.
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Adipose-Derived Stem Cells - Are They the Optimal Cell Source for Urinary Tract Regeneration? (Chapter 1)Book Chapter
D, Eberli (Ed.): Cells and Biomaterials in Regenerative Medicine, InTech, 2014, ISBN: 9789535117315.
Adipose-derived stem cells for treatment of lower genitourinary tract dysfunctionJournal Article
J Stem Cell Res Ther, 4 (4), 2014.
Using human umbilical cord cells for tissue engineering: a comparison with skin cells.Journal Article
Differentiation, 87 (3-4), 2014.
Human epithelial stem cells persist within tissue-engineered skin produced by the self-assembly approach.Journal Article
Tissue Eng Part A, 19 (7-8), 2013.
Dynamic culture induces a cell type-dependent response impacting on the thickness of engineered connective tissues.Journal Article
J Tissue Eng Regen Med, 7 (4), 2013.
Harvesting the potential of the human umbilical cord: isolation and characterisation of four cell types for tissue engineering applications.Journal Article
Cells Tissues Organs, 197 (1), 2013.
Adipose stem cells, Tissue engineering and solid organ transplantation and regenerationBook Chapter
IllouzYGandStero, A (Ed.): Adipose stem cells and regenerative medicine, pp. 229-243, Springer-Verlag, 2011.
Stem cells of the skin and cornea: their clinical applications in regenerative medicine.Journal Article
Curr Opin Organ Transplant, 16 (1), 2011.
Cell sheet technology for tissue engineering: the self-assembly approach using adipose-derived stromal cells.Journal Article
Methods Mol Biol, 702 , 2011.
Considerations in the choice of a skin donor site for harvesting keratinocytes containing a high proportion of stem cells for culture in vitro.Journal Article
Burns, 37 (3), 2011.
- Design and testing of a next-generation tissue-engineered living human heart valve, from 2020-03-31 to 2022-03-30
- Réseau de thérapie cellulaire, tissulaire et génique du Québec, from 2019-04-01 to 2024-03-31
- Roles of matricryptins (bioactive extracellular matrix fragments) in adipose tissue, from 2020-04-01 to 2025-03-31
- Tissue Engineering of a functional Urethra for Autologous Reconstruction, from 2018-04-01 to 2022-03-31
Recently finished projects
- (FRSQ 85117)La guérison des plaies cutanées par utilisation des cellules souches, from 2019-04-09 to 2020-03-31
- Caractérisation de la variabilité fonctionelle des cellules souchez mésenchymateuses humaines dérivée des tissus adipeux de patints sains ou dysmétaboliques, from 2019-04-01 to 2020-03-31
- Centre de recherche en arthrite de l'Université Laval, from 2019-01-01 to 2020-01-31
- Projet de recherche portant sur la guérison des plaies cutanées par l'utilisation des cellules souches et de nouvelles thérapies combinées, from 2020-04-16 to 2021-04-15
- Régulation de l'angiogenèse par lesmatricryptines dans le tissu adipeux humain, from 2017-04-01 to 2019-09-30
- Variabilité fonctionnelle des cellules souches mésenchymateuses du tissu adipeux de patients sains ou dysmétaboliques., from 2019-04-01 to 2020-03-31