Pr. Fradette has been a regular researcher at the CRCHU de Québec-Laval University since 2005, and a full professor of the Department of Surgery of the Laval University School of Medicine. After a bachelor’s degree in biochemistry (1991-1994, U Laval) and a PhD in cellular and molecular biology focused on the study of skin epithelial stem cells (1994-1999, U Laval, with L. Germain, LOEX), she acquired a specialization in gene therapy during her postdoctoral fellowship at the University of Pittsburgh (1999-2004, with J. Glorioso III, U Pittsburgh, PA, USA). Her current research program is based on the use of human adipose-derived stromal/stem cells in regenerative medicine. Since September of 2015, she has been the director of the ThéCell network, the province of Quebec network for cell and tissue therapy supported by the FRQS.
Development of human reconstructed tissues as 3D discovery models and for clinical applications
Pr. Fradette’s current research program is based on the use of a new and abundant source of multipotential cells: the adipose-derived stromal/stem cells (ASCs). Using tissue-engineering techniques, her team uses ASCs to recreate human tissue which is needed in reconstructive surgery, such as skin, adipose, and bone-like tissue. For example, human adipose substitutes recreating the 3D tissular context in vitro are useful discovery tools for pharmaco-toxicological studies on the metabolic regulation of human adipocytes. Moreover, adipose tissues are reconstructed with the important goal of being used in the clinic as soft-tissue fillers to repair tissue loss, in cases of deep burns or tumor removal (including mastectomy). The resulting tissues are all human, and devoid of exogenous biomaterials.
Her research interests include a better understanding of the cellular interactions between adipocytes and endothelial cells, the effects of inflammatory conditions on blood capillaries and adipocyte metabolism, the impact of human ECM components on the adipogenic differentiation of ASCs, and how MMPs can influence tissue remodeling, under normal and inflammatory conditions. Current studies performed in her laboratory aim at promoting the vascularisation of thick reconstructed tissues produced under xenogenic-free culture conditions for clinical applications. Ongoing preclinical studies assess the efficacy of tissue-engineered biological dressings to accelerate the healing of skin wounds.
In collaboration with D.r M. Fortin and Dr. FA. Auger, her team also produces bone-like substitutes from ASCs.
Local R-221 LOEX / CMDGT
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In Vitro Prevascularization of Self-Assembled Human Bone-Like Tissues and Preclinical Assessment Using a Rat Calvarial Bone Defect Model.Journal Article
Materials (Basel), 14 (8), 2021.
Investigation of Omega-3 Polyunsaturated Fatty Acid Biological Activity in a Tissue-Engineered Skin Model Involving Psoriatic Cells.Journal Article
J Invest Dermatol, 2021.
Human Organ-Specific 3D Cancer Models Produced by the Stromal Self-Assembly Method of Tissue Engineering for the Study of Solid Tumors.Journal Article
Biomed Res Int, 2020 , 2020.
Modulation of the Lipid Profile of Reconstructed Skin Substitutes after Essential Fatty Acid Supplementation Affects Testosterone Permeability.Journal Article
Cells, 8 (10), 2019.
Linoleic acid supplementation of cell culture media influences the phospholipid and lipid profiles of human reconstructed adipose tissue.Journal Article
PLoS One, 14 (10), 2019.
Isolation and Culture of Human Dermal Microvascular Endothelial Cells.Journal Article
Methods Mol Biol, 1993 , 2019.
Strategies to promote the vascularization of skin substitutes after transplantation (Chapter 8)Book Chapter
AP, Marques; RP, Pirraco; MT, Cerqueira; RL, Reis (Ed.): Skin Tissue Models, pp. 177-200, London Academic Press, 2018, ISBN: 9780128105450.
Self-assembled human osseous cell sheets as living biopapers for the laser-assisted bioprinting of human endothelial cells.Journal Article
Biofabrication, 10 (3), 2018.
Corrigendum to "Inflammatory cytokine profiles in visceral and subcutaneous adipose tissues of obese patients undergoing bariatric surgery reveal lack of correlation with obesity or diabetes" EBioMedicine 30C (2018) 237-247.Journal Article
EBioMedicine, 37 , 2018.
Inflammatory Cytokine Profiles in Visceral and Subcutaneous Adipose Tissues of Obese Patients Undergoing Bariatric Surgery Reveal Lack of Correlation With Obesity or Diabetes.Journal Article
EBioMedicine, 30 , 2018.
- Design and testing of a next-generation tissue-engineered living human heart valve, from 2020-03-31 to 2022-03-30
- Réseau de thérapie cellulaire, tissulaire et génique du Québec, from 2019-04-01 to 2024-03-31
- Roles of matricryptins (bioactive extracellular matrix fragments) in adipose tissue, from 2020-04-01 to 2025-03-31
- Tissue Engineering of a functional Urethra for Autologous Reconstruction, from 2018-04-01 to 2022-03-31
Recently finished projects
- (FRSQ 85117)La guérison des plaies cutanées par utilisation des cellules souches, from 2019-04-09 to 2020-03-31
- Caractérisation de la variabilité fonctionelle des cellules souchez mésenchymateuses humaines dérivée des tissus adipeux de patints sains ou dysmétaboliques, from 2019-04-01 to 2020-03-31
- Centre de recherche en arthrite de l'Université Laval, from 2019-01-01 to 2020-01-31
- Projet de recherche portant sur la guérison des plaies cutanées par l'utilisation des cellules souches et de nouvelles thérapies combinées, from 2020-04-16 to 2021-04-15
- Régulation de l'angiogenèse par lesmatricryptines dans le tissu adipeux humain, from 2017-04-01 to 2019-09-30
- Variabilité fonctionnelle des cellules souches mésenchymateuses du tissu adipeux de patients sains ou dysmétaboliques., from 2019-04-01 to 2020-03-31