Christian Salesse is a full-time researcher at the Centre de recherche du CHU de Québec – Laval University, and full professor in Laval University’s Department of Ophthalmology. He graduated in biophysics from Université du Québec à Trois-Rivières (UQTR). He was then a postdoctoral fellow at the Johannes-Gutenberg Universität in Mainz, Germany. He then became professor at the UQTR for 14 years until he moved on to work at Laval University, in 2002. He has been a fellow from Fonds de Recherche – Québec Santé (FRQS) from the Junior 1 to the National level. He has also been Adjunct Professor at Jilin University in Changchun, China since 1995. He was an invited professor at several universities in France and Portugal.

His expertise is in the field of visual phototransduction and visual cycle, and the proteins involved in these processes. These processes require the binding of most of these proteins to membranes. We therefore perform membrane binding measurements of proteins. We are using simple model membranes such as lipid monolayers and bilayers to characterize their membrane binding. Protein binding to monolayers of saturated and highly polyunsaturated neutral or negatively charged lipids, that are typical of photoreceptors, is characterized by performing measurements of maximum insertion pressure, fluorescence microscopy, ellipsometry, as well as infrared spectroscopy (PM-IRRAS which also allows to determine protein orientation). Steady state fluorescence spectroscopy and fluorescence lifetime are used to characterize protein binding to lipid bilayers. The proteins we are studying also often have enzymatic activities; we have thus developed methods to characterize the activity of some of these enzymes. Moreover, we use nuclear magnetic resonance to determine the structure of a protein involved in the visual cycle.

Hôpital Saint-Sacrement
1050, chemin Sainte-Foy
CUO-Recherche
K2-02
Québec, Québec
Canada G1S 4L8
+1 418-525-4444, extension 87569
christian.salesse@crchude... christian.salesse@fmed.ul...

Latest news

125 entries « ‹ 3 of 13 › »

Lhor M, Methot M, Horchani H, Salesse C

Structure of the N-terminal segment of human retinol dehydrogenase 11 and its preferential lipid binding using model membranes

Journal Article

Biochim Biophys Acta, 1848 (3), 2015.

Abstract | Links:

Demers E, Boisselier E, Horchani H, Blaudez D, Calvez P, Cantin L, Belley N, Champagne S, Desbat B, Salesse C

Lipid Selectivity, Orientation, and Extent of Membrane Binding of Nonacylated RP2

Journal Article

Biochemistry, 54 (16), 2015.

Abstract | Links:

Lhor M, Salesse C

Retinol dehydrogenases: membrane-bound enzymes for the visual function

Journal Article

Biochem Cell Biol, 92 (6), 2014.

Abstract | Links:

Lhor M, Bernier SC, Horchani H, Bussieres S, Cantin L, Desbat B, Salesse C

Comparison between the behavior of different hydrophobic peptides allowing membrane anchoring of proteins

Journal Article

Adv Colloid Interface Sci, 207 , 2014.

Abstract | Links:

Horchani H, Bussieres S, Cantin L, Lhor M, Laliberte-Gemme JS, Breton R, Salesse C

Enzymatic activity of Lecithin:retinol acyltransferase: a thermostable and highly active enzyme with a likely mode of interfacial activation

Journal Article

Biochim Biophys Acta, 1844 (6), 2014.

Abstract | Links:

Potvin-Fournier K, Lefevre T, Picard-Lafond A, Valois-Paillard G, Cantin L, Salesse C, Auger M

The thermal stability of recoverin depends on calcium binding and its myristoyl moiety as revealed by infrared spectroscopy

Journal Article

Biochemistry, 53 (1), 2014.

Abstract | Links:

Zhang C, Pan T, Salesse C, Zhang D, Miao L, Wang L, Gao Y, Xu J, Dong Z, Luo Q, Liu J

Reversible Ca(2+) switch of an engineered allosteric antioxidant selenoenzyme

Journal Article

Angew Chem Int Ed Engl, 53 (49), 2014.

Abstract | Links:

Boisselier E, Calvez P, Demers E, Cantin L, Salesse C

Effect of oxidation of polyunsaturated phospholipids on the binding of proteins in monolayers

Journal Article

Colloids Surf B Biointerfaces, 109 , 2013.

Abstract | Links:

Lake J, Zaniolo K, Gaudreault M, Carrier P, Deschambault A, Bazin R, Germain L, Salesse C, Guerin SL

Expression of the α5 integrin gene in corneal epithelial cells cultured on tissue-engineered human extracellular matrices

Journal Article

Biomaterials, 34 (27), 2013.

Abstract | Links:

Bussieres S, Cantin L, Desbat B, Salesse C

Binding of a truncated form of lecithin:retinol acyltransferase and its N- and C-terminal peptides to lipid monolayers

Journal Article

Langmuir, 28 (7), 2012.

Abstract | Links:

125 entries « ‹ 3 of 13 › »
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Active projects

  • A recombinant soluble NTPDase8 as a novel biological treatment for inflammatory bowel disease, from 2021-10-01 to 2026-09-30
  • Implication de diverses protéines dans la fonction des photorécepteurs visuels, from 2023-04-01 to 2028-03-31
  • Journée annuelle de la recherche en ophtalmologie , from 2019-04-23 to 2025-03-31

Recently finished projects

  • Caractérisation de la liaison membranaire de la rhodopsine kinase, from 2023-01-15 to 2023-06-10
  • Implication de diverses protéines dans la fonction des photorécepteurs visuels, from 2017-04-01 to 2023-03-31
  • PROTEO, le regroupement québécois de recherche sur la fonction, l'ingénierie et les applications des protéines (PROTEO), from 2015-04-01 to 2023-03-31
Data provided by the Université Laval research projects registery