Christian Salesse is a full-time researcher at the Centre de recherche du CHU de Québec – Laval University, and full professor in Laval University’s Department of Ophthalmology. He graduated in biophysics from Université du Québec à Trois-Rivières (UQTR). He was then a postdoctoral fellow at the Johannes-Gutenberg Universität in Mainz, Germany. He then became professor at the UQTR for 14 years until he moved on to work at Laval University, in 2002. He has been a fellow from Fonds de Recherche – Québec Santé (FRQS) from the Junior 1 to the National level. He has also been Adjunct Professor at Jilin University in Changchun, China since 1995. He was an invited professor at several universities in France and Portugal.
His expertise is in the field of visual phototransduction and visual cycle, and the proteins involved in these processes. These processes require the binding of most of these proteins to membranes. We therefore perform membrane binding measurements of proteins. We are using simple model membranes such as lipid monolayers and bilayers to characterize their membrane binding. Protein binding to monolayers of saturated and highly polyunsaturated neutral or negatively charged lipids, that are typical of photoreceptors, is characterized by performing measurements of maximum insertion pressure, fluorescence microscopy, ellipsometry, as well as infrared spectroscopy (PM-IRRAS which also allows to determine protein orientation). Steady state fluorescence spectroscopy and fluorescence lifetime are used to characterize protein binding to lipid bilayers. The proteins we are studying also often have enzymatic activities; we have thus developed methods to characterize the activity of some of these enzymes. Moreover, we use nuclear magnetic resonance to determine the structure of a protein involved in the visual cycle.
1050, chemin Sainte-Foy
CUO-Recherche
K2-02
Québec, Québec
Canada G1S 4L8
- Cantin, LineEmployeeHôpital Saint-Sacrement+1 418-682-7661+1 418-682-8000line.cantin@crchudequebec.ulaval.caline.cantin@fmed.ulaval.ca
1050, chemin Sainte-Foy
Québec, Québec
Canada G1S 4L8 - Gosselin, OlivierMaster studentHôpital Saint-Sacrement+1 418-525-4444, extension 52363olivier.gosselin@crchudequebec.ulaval.ca
1050, chemin Sainte-Foy
Québec, QC
Canada G1S 4L8 - Millette, Marc-AntoineDoctoral studentHôpital Saint-Sacrementmarc-antoine.millette.1@ulaval.camarc-antoine.millette@crchudequebec.ulaval.ca
1050 Chemin Sainte-Foy
Québec, QC
Canada G1S 4L8 - Roy, SarahDoctoral studentHôpital Saint-Sacrementsarah.roy.4@ulaval.casarah.roy@crchudequebec.ulaval.ca
1050 Chemin Sainte-Foy
JS2-05
Québec, QC
Canada G1S 4L8 - Targon Campana, PatriciaEmployeeHôpital Saint-Sacrement
1050 Chemin Sainte-Foy
H2-06
Québec, QC
Canada G1S 4L8
[Physiology of the visual retinal signal: From phototransduction to the visual cycle]
Journal ArticleJ Fr Ophtalmol, 40 (3), 2017.
How to gather useful and valuable information from protein binding measurements using Langmuir lipid monolayers
Journal ArticleAdv Colloid Interface Sci, 243 , 2017.
Membrane fluidity is a driving force for recoverin myristoyl immobilization in zwitterionic lipids
Journal ArticleBiochem Biophys Res Commun, 490 (4), 2017.
Physiologie du signal visuel rétinien : de la phototransduction jusqu’au cycle visuel
Book ChapterEM| consulte. Ophtalmologie, 13 , pp. [Article 21-026-K-10], Issy les Moulineaux, Elsevier Masson SAS, 2016.
Phosphatidylserine Allows Observation of the Calcium-Myristoyl Switch of Recoverin and Its Preferential Binding
Journal ArticleJ Am Chem Soc, 138 (41), 2016.
Discriminating Lipid- from Protein-Calcium Binding To Understand the Interaction between Recoverin and Phosphatidylglycerol Model Membranes
Journal ArticleBiochemistry, 55 (24), 2016.
How To Decipher Protein and Peptide Selectivity for Lipids in Monolayers
Book ChapterWang C, Leblanc RM (Ed.): Recent Progress in Colloid and Surface Chemistry with Biological Applications, 1215 , pp. 109-128, American Chemical Society, 2015, ISBN: 9780841231207.
Structure and binding of the C-terminal segment of R9AP to lipid monolayers
Journal ArticleLangmuir, 31 (6), 2015.
Functional Impact of Collagens on the Activity Directed by the Promoter of the α5 Integrin Subunit Gene in Corneal Epithelial Cells
Journal ArticleInvest Ophthalmol Vis Sci, 56 (11), 2015.
Characterization of the human α9 integrin subunit gene: Promoter analysis and transcriptional regulation in ocular cells
Journal ArticleExp Eye Res, 135 , 2015.
Active projects
- A recombinant soluble NTPDase8 as a novel biological treatment for inflammatory bowel disease, from 2021-10-01 to 2026-09-30
- Implication de diverses protéines dans la fonction des photorécepteurs visuels, from 2023-04-01 to 2028-03-31
- Journée annuelle de la recherche en ophtalmologie , from 2019-04-23 to 2025-03-31
Recently finished projects
- Caractérisation de la liaison membranaire de la rhodopsine kinase, from 2023-01-15 to 2023-06-10
- Implication de diverses protéines dans la fonction des photorécepteurs visuels, from 2017-04-01 to 2023-03-31
- PROTEO, le regroupement québécois de recherche sur la fonction, l'ingénierie et les applications des protéines (PROTEO), from 2015-04-01 to 2023-03-31