Christian Salesse is a full-time researcher at the Centre de recherche du CHU de Québec – Laval University, and full professor in Laval University’s Department of Ophthalmology. He graduated in biophysics from Université du Québec à Trois-Rivières (UQTR). He was then a postdoctoral fellow at the Johannes-Gutenberg Universität in Mainz, Germany. He then became professor at the UQTR for 14 years until he moved on to work at Laval University, in 2002. He has been a fellow from Fonds de Recherche – Québec Santé (FRQS) from the Junior 1 to the National level. He has also been Adjunct Professor at Jilin University in Changchun, China since 1995. He was an invited professor at several universities in France and Portugal.

His expertise is in the field of visual phototransduction and visual cycle, and the proteins involved in these processes. These processes require the binding of most of these proteins to membranes. We therefore perform membrane binding measurements of proteins. We are using simple model membranes such as lipid monolayers and bilayers to characterize their membrane binding. Protein binding to monolayers of saturated and highly polyunsaturated neutral or negatively charged lipids, that are typical of photoreceptors, is characterized by performing measurements of maximum insertion pressure, fluorescence microscopy, ellipsometry, as well as infrared spectroscopy (PM-IRRAS which also allows to determine protein orientation). Steady state fluorescence spectroscopy and fluorescence lifetime are used to characterize protein binding to lipid bilayers. The proteins we are studying also often have enzymatic activities; we have thus developed methods to characterize the activity of some of these enzymes. Moreover, we use nuclear magnetic resonance to determine the structure of a protein involved in the visual cycle.

Hôpital Saint-Sacrement
1050, chemin Sainte-Foy
CUO-Recherche
K2-02
Québec, Québec
Canada G1S 4L8
+1 418-525-4444, extension 87569
christian.salesse@crchude... christian.salesse@fmed.ul...

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125 entries « ‹ 12 of 13 › »

Salesse C, Ducharme D, Leblanc RM, Boucher F

Estimation of disk membrane lateral pressure and molecular area of rhodopsin by the measurement of its orientation at the nitrogen-water interface from an ellipsometric study

Journal Article

Biochemistry, 29 (19), 1990.

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Ducharme D, Max JJ, Salesse C, Leblanc RM

Ellipsometric study of the physical states of phosphatidylcholines at the air-water interface

Journal Article

J Phys Chem, 94 (5), 1990.

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Grainger DW, Reichert A, Ringsdorf H, Salesse C

Hydrolytic action of phospholipase A2 in monolayers in the phase transition region: direct observation of enzyme domain formation using fluorescence microscopy

Journal Article

Biochim Biophys Acta, 1023 (3), 1990.

Abstract | Links:

Grainger DW, Reichert A, Ringsdorf H, Salesse C, Davies DE, Lloyd JB

Mixed monolayers of natural and polymeric phospholipids: structural characterization by physical and enzymatic methods

Journal Article

Biochim Biophys Acta, 1022 (2), 1990.

Abstract | Links:

Ahlers M, Blankenburg R, Grainger DW, Meller P, Ringsdorf H, Salesse C

Specific recognition and formation of two- dimensional streptavidin domains in monolayers: applications to molecular devices

Journal Article

Thin Solid Films, 180 (1-2), 1989.

Abstract | Links:

Salesse C, Lamarche F, Leblanc RM

The binding of G-protein to rod outer segment phospholipids at the nitrogen-water interface

Journal Article

Biochem Cell Biol, 67 (8), 1989.

Abstract | Links:

Blankenburg R, Meller P, Ringsdorf H, Salesse C

Interaction between biotin lipids and streptavidin in monolayers: formation of oriented two-dimensional protein domains induced by surface recognition

Journal Article

Biochemistry, 28 (20), 1989.

Abstract | Links:

Salesse C, Leblanc RM, Kito Y

Lipid contamination of disks depends on rod outer-segment purity

Journal Article

Exp Eye Res, 46 (2), 1988.

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Auger M, Salesse C, Tancède P

The effect of pH on the interactions in mixed monolayers between phosphatidylserine and all-Trans retinal

Journal Article

J Colloid Interface Sci, 123 (1), 1988.

Abstract | Links:

Salesse C, Ducharme D, Leblanc RM

Direct evidence for the formation of a monolayer from a bilayer. An ellipsometric study at the nitrogen-water interface

Journal Article

Biophys J, 52 (2), 1987.

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125 entries « ‹ 12 of 13 › »
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Active projects

  • A recombinant soluble NTPDase8 as a novel biological treatment for inflammatory bowel disease, from 2021-10-01 to 2026-09-30
  • Implication de diverses protéines dans la fonction des photorécepteurs visuels, from 2023-04-01 to 2028-03-31
  • Journée annuelle de la recherche en ophtalmologie , from 2019-04-23 to 2025-03-31

Recently finished projects

  • Caractérisation de la liaison membranaire de la rhodopsine kinase, from 2023-01-15 to 2023-06-10
  • Implication de diverses protéines dans la fonction des photorécepteurs visuels, from 2017-04-01 to 2023-03-31
  • PROTEO, le regroupement québécois de recherche sur la fonction, l'ingénierie et les applications des protéines (PROTEO), from 2015-04-01 to 2023-03-31
Data provided by the Université Laval research projects registery