Christian Salesse is a full-time researcher at the Centre de recherche du CHU de Québec – Laval University, and full professor in Laval University’s Department of Ophthalmology. He graduated in biophysics from Université du Québec à Trois-Rivières (UQTR). He was then a postdoctoral fellow at the Johannes-Gutenberg Universität in Mainz, Germany. He then became professor at the UQTR for 14 years until he moved on to work at Laval University, in 2002. He has been a fellow from Fonds de Recherche – Québec Santé (FRQS) from the Junior 1 to the National level. He has also been Adjunct Professor at Jilin University in Changchun, China since 1995. He was an invited professor at several universities in France and Portugal.

His expertise is in the field of visual phototransduction and visual cycle, and the proteins involved in these processes. These processes require the binding of most of these proteins to membranes. We therefore perform membrane binding measurements of proteins. We are using simple model membranes such as lipid monolayers and bilayers to characterize their membrane binding. Protein binding to monolayers of saturated and highly polyunsaturated neutral or negatively charged lipids, that are typical of photoreceptors, is characterized by performing measurements of maximum insertion pressure, fluorescence microscopy, ellipsometry, as well as infrared spectroscopy (PM-IRRAS which also allows to determine protein orientation). Steady state fluorescence spectroscopy and fluorescence lifetime are used to characterize protein binding to lipid bilayers. The proteins we are studying also often have enzymatic activities; we have thus developed methods to characterize the activity of some of these enzymes. Moreover, we use nuclear magnetic resonance to determine the structure of a protein involved in the visual cycle.

Hôpital Saint-Sacrement
1050, chemin Sainte-Foy
CUO-Recherche
K2-02
Québec, Québec
Canada G1S 4L8
125 entries « 1 of 13 »

Lachance A, Hébert M, Salesse C, Bourgault S, Dirani A

Analysis of Molecular Genetic Testing Referrals for Inherited Retinal Dystrophies in a Quebec Tertiary Care Center Over a Decade

Journal Article

Clin Ophthalmol, 16 , 2022.

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Millette MA, Roy S, Salesse C

Farnesylation and lipid unsaturation are critical for the membrane binding of the C-terminal segment of G-Protein Receptor Kinase 1

Journal Article

Colloids Surf B Biointerfaces, 211 , 2022.

Abstract | Links:

Hädicke A, Coutinho A, Roy S, Otis F, Lhor M, Cantin L, Prieto M, Voyer N, Salesse C

Membrane binding properties of the C-terminal segment of retinol dehydrogenase 8

Journal Article

Biochim Biophys Acta Biomembr, 1863 (9), 2021.

Abstract | Links:

Bernier SC, Millette MA, Roy S, Cantin L, Coutinho A, Salesse C

Structural information and membrane binding of truncated RGS9-1 Anchor Protein and its C-terminal hydrophobic segment

Journal Article

Biochim Biophys Acta Biomembr, 1863 (4), 2021.

Abstract | Links:

Schmidt TF, Riske KA, Caseli L, Salesse C

Dengue fusion peptide in Langmuir monolayers: A binding parameter study

Journal Article

Biophys Chem, 271 , 2021.

Abstract | Links:

Gauthier ME, Roy S, Cantin L, Salesse C

Comparison between the enzymatic activity, structure and substrate binding of mouse and human lecithin retinol acyltransferase

Journal Article

Biochem Biophys Res Commun, 519 (4), 2019.

Abstract | Links:

Potvin-Fournier K, Valois-Paillard G, Gagnon MC, Lefevre T, Audet P, Cantin L, Paquin JF, Salesse C, Auger M

Novel approaches to probe the binding of recoverin to membranes

Journal Article

Eur Biophys J, 47 (6), 2018.

Abstract | Links:

Bernier SC, Cantin L, Salesse C

Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags

Journal Article

Protein Expr Purif, 152 , 2018.

Abstract | Links:

Bernier SC, Morency LP, Najmanovich R, Salesse C

Identification of an alternative translation initiation site in the sequence of the commonly used Glutathione S-Transferase tag

Journal Article

J Biotechnol, 286 , 2018.

Abstract | Links:

Cyr N, Smith TK, Boisselier E, Leroux LP, Kottarampatel AH, Davidsen A, Salesse C, Jardim A

The hydrophobic region of the leishmania peroxin 14: requirements for association with a glycosome mimetic membrane

Journal Article

Biochem J, 475 (2), 2018.

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125 entries « 1 of 13 »
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Active projects

  • A recombinant soluble NTPDase8 as a novel biological treatment for inflammatory bowel disease, from 2021-10-01 to 2026-09-30
  • Implication de diverses protéines dans la fonction des photorécepteurs visuels, from 2017-04-01 to 2023-03-31
  • PROTEO, le regroupement québécois de recherche sur la fonction, l'ingénierie et les applications des protéines (PROTEO), from 2015-04-01 to 2023-03-31

Recently finished projects

  • Déterminants moléculaires de la lécithine rétinol acyltransférase responsables de la dégénérescence des photorécepteurs, from 2016-07-01 to 2021-06-30
  • Journée annuelle de la recherche en ophtalmologie , from 2019-04-23 to 2021-03-31
Data provided by the Université Laval research projects registery