Data production ranges from 1 to 20 million paired-end reads per sequencing run. It is widely used for metagenomic analyses (16S and ITS sequencing) because it allows for 300 base pair reads.
| Nano | Micro | V2 | V3 | |
| Per Flowcell* | ||||
| Clusters/Line – MAX | 1 M | 4 M | 12 M | 20 M |
| 2X 50 bp | na | na | 1.2 Gb | na |
| 2X 150 bp | 0.3 Gb | 1.2 Gb | 3.6 Gb | 6.0 Gb |
| 2X 250 bp | na | na | 6.0 Gb | na |
| 2X 300 bp | na | na | na | 12 Gb |
*Yield may vary depending on the library type.