The in vitro fluorescence imaging system allows to produce images to evaluate the distribution of the specific signal of cell colonies (well plates, Petri dishes), partial or entire organs, the dynamics of the expression of a protein even through the skull. Also, this technology is quick to generate images even with a large number of animals, is very sensitive to weak signals and permits to overcome non-specific noise. Its greatest asset is the normalization of the detected signal which allows the comparison of measurements with experiments carried out on instruments of the same technology in other laboratories.