Dr. Sabine Elowe received her PhD from the University of Toronto in 2005, where she studied tyrosine kinase receptor signaling with Dr. Tony Pawson at the Lunenfeld-Tanenbaum Research Institute (formerly the Samuel Lunenfeld Research Institute). She then completed a postdoctoral internship at the Max Planck Institute of Biochemistry in Munich, Germany where she worked from 2005 to 2009 on the molecular mechanisms of cell division with Dr. Erich Nigg. She established her own laboratory at the CHU de Québec/Laval University in 2010. She is the recipient of numerous awards, including a CIHR New Investigator Salary Award, and FRQS Salary Awards.

The research

Aneuploidy is a pathological condition defined by an aberrant number of chromosomes. Dr. Elowe’s lab is interested in the characterization of signaling pathways and molecular mechanisms of action that ensure the precision of cell division, and thus the prevention of aneuploidy. Specifically, we are interested in a highly conserved kinase group known as the “Spinde Assembly Checkpoint” or “SAC” kinases, which collectively provides normal cell division by two distinct mechanisms: 1) ensuring the attachment of the chromosome to the cell division apparatus 2) delaying the division process until all elements of the division system are in position.

Our research focuses on the following questions:

  1. What are the mechanisms of activation and inactivation of SAC kinases?
  2. What are the endogenous substrates of SAC kinases?
  3. Is SAC differentially regulated in cell lines with chromosomal instability?
  4. How does aneuploidy contribute to cancer?

We will use a multidisciplinary approach to identify the signaling pathways involved, including RNA interference coupled with restoration approaches, mass spectrometry, generation and use of phospho-specific antibodies, indirect immunofluorescence, and high resolution video microscopy.

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Wang LH, Yen CJ, Li TN, Elowe S, Wang WC

Sgo1 is a potential therapeutic target for hepatocellular carcinoma.

Journal Article

Oncotarget, 6 (4), pp. 2023-33, 2015, ISSN: 1949-2553.

Abstract | Links:

Magron A, Elowe S, Carreau M

The Fanconi Anemia C Protein Binds to and Regulates Stathmin-1 Phosphorylation.

Journal Article

PLoS ONE, 10 (10), pp. e0140612, 2015.

Abstract | Links:

Ghongane P, Kapanidou M, Asghar A, Elowe S, Bolanos-Garcia VM

The dynamic protein Knl1 - a kinetochore rendezvous.

Journal Article

J Cell Sci, 127 (Pt 16), pp. 3415-23, 2014, ISSN: 0021-9533.

Abstract | Links:

Murphy JM, Zhang Q, Young SN, Reese ML, Bailey FP, Eyers PA, Ungureanu D, Hammaren H, Silvennoinen O, Varghese LN, Chen K, Tripaydonis A, Jura N, Fukuda K, Qin J, Nimchuk Z, Mudgett MB, Elowe S, Gee CL, Liu L, Daly RJ, Manning G, Babon JJ, Lucet IS

A robust methodology to subclassify pseudokinases based on their nucleotide-binding properties.

Journal Article

Biochem J, 457 (2), pp. 323-34, 2014, ISSN: 0264-6021.

Abstract | Links:

Kasahara K, Goto H, Izawa I, Kiyono T, Watanabe N, Elowe S, Nigg EA, Inagaki M

PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3γ and is required for metaphase-anaphase transition.

Journal Article

Nat Commun, 4 , pp. 1882, 2013, ISSN: 2041-1723.

Abstract | Links:

Thebault P, Chirgadze DY, Dou Z, Blundell TL, Elowe S, Bolanos-Garcia VM

Structural and functional insights into the role of the N-terminal Mps1 TPR domain in the SAC (spindle assembly checkpoint).

Journal Article

Biochem J, 448 (3), pp. 321-8, 2012, ISSN: 0264-6021.

Abstract | Links:

Lee S, Thebault P, Freschi L, Beaufils S, Blundell TL, Landry CR, Bolanos-Garcia VM, Elowe S

Characterization of spindle checkpoint kinase Mps1 reveals domain with functional and structural similarities to tetratricopeptide repeat motifs of Bub1 and BubR1 checkpoint kinases.

Journal Article

J Biol Chem, 287 (8), pp. 5988-6001, 2012, ISSN: 0021-9258.

Abstract | Links:

Rojas AM, Santamaria A, Malik R, Jensen TS, Korner R, Morilla I, de Juan D, Krallinger M, Hansen DA, Hoffmann R, Lees J, Reid A, Yeats C, Wehner A, Elowe S, Clegg AB, Brunak S, Nigg EA, Orengo C, Valencia A, Ranea JA

Uncovering the molecular machinery of the human spindle--an integration of wet and dry systems biology.

Journal Article

PLoS ONE, 7 (3), pp. e31813, 2012.

Abstract | Links:

Elowe S

Bub1 and BubR1: at the interface between chromosome attachment and the spindle checkpoint.

Journal Article

Mol Cell Biol, 31 (15), pp. 3085-93, 2011, ISSN: 0270-7306.

Abstract | Links:

Dou Z, von Schubert C, Korner R, Santamaria A, Elowe S, Nigg EA

Quantitative mass spectrometry analysis reveals similar substrate consensus motif for human Mps1 kinase and Plk1.

Journal Article

PLoS ONE, 6 (4), pp. e18793, 2011.

Abstract | Links:

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Signaler des ajouts ou des modifications

Active projects

  • Analyse protéomique des réseaux de signalisation dépendants des récepteurs tyrosine kinase de la famille EPH et de leurs fonctions dans la ségrégation cellulaire et la polarité épitheliale, Subvention, Fonds de recherche du Québec - Nature et technologies, Projet de recherche en équipe, from 2018-04-01 to 2021-03-31
  • Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
  • Centre de recherche sur le cancer, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 1996-05-01 to 2022-06-13
  • Phosphotyrosine signalling during mitosis., Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2016-04-01 to 2022-03-31
  • PROTEO, le regroupement québécois de recherche sur la fonction, l'ingénierie et les applications des protéines (PROTEO), Subvention, Fonds de recherche du Québec - Nature et technologies, Regroupements stratégiques NT, from 2015-04-01 to 2022-03-31
  • Regroupement québécois de recherche sur la fonction, la structure et l'ingénierie des protéines, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 2014-05-01 to 2023-04-03
  • Regulation of chromosome cohesion and genome stability by a novel Histone modification, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2018-04-01 to 2023-03-31
  • Towards a comprehensive model of Spindle Assembly Checkpoint silencing, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2017-04-01 to 2022-03-31

Recently finished projects

  • Exploring the functions of the Bub1 and BubR1 kinases during mitosis., Subvention, Instituts de recherche en santé du Canada, Subvention de fonctionnement, from 2011-10-01 to 2020-03-31
  • High throughput analysis of protein interactions and modifications using peptide arrays, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions d'outils et d'instruments de recherche (OIR), from 2019-03-28 to 2020-03-31
  • Les kinases mitotiques et la régulation de l'aneuploïdie et cancer, Subvention, Fonds de recherche du Québec - Santé, Chercheur-boursier Juniors 1 et 2, Seniors, from 2017-07-01 to 2020-06-30
Data provided by the Université Laval research projects registery