Dr. Roxane Pouliot is a regular researcher at the CHU Research Centre of Quebec-Laval University, and Professor at the Faculty of Pharmacy of Laval University. As a regular member, she is also part of the Centre for Advanced Materials Research (CAMaR), as well as the Centre Québécois on Functional Materials (CQMF). Her research interests focus on a better understanding of the structures and functions of skin tissue through advanced models of tissue reconstruction. Her team recently developed an immunocompetent psoriatic skin replacement model reconstructed from human cells, a major breakthrough in this area.

Dr. Pouliot is the recipient of Laval University’s 2016 Graduate Teaching and Coaching Award.

Understanding the complexity of structures and functions of skin tissue

In vitro skin diffusion tests are one of the first steps in the development of a transdermal drug. To this end, a robust model of cutaneous biomaterial is necessary for the precise study of the diffusion mechanisms of therapeutic molecules. Advanced lipidomic characterization of tissue-engineered samples is essential for understanding the skin lipid domain, which is complex and poorly reproduced in vitro. However, the biomimetic quality currently developed within our research team allows us to consider improving the models to obtain the qualities found within a native skin, whether healthy or pathological.

Modelling psoriasis and developing new pharmacological strategies

Cutaneous diseases such as psoriasis are complex diseases whose development and persistence involve several cellular processes and cell types. In recent years, our research team has developed a world-unique model of reconstructed psoriatic skin. Through tissue engineering, it becomes possible to reconstruct, according to the self-assembly method, a completely autologous pathological skin, that is to say without exogenous material, and composed of both a dermis and an epidermis. This model of pathological skin provides us with the opportunity to study separately the players involved in the development of psoriasis, and therefore becomes a powerful tool in the mechanistic analysis of this complex pathology. Distinctly, the latter is three-dimensional, designed from psoriatic human cells, in addition to developing in a highly physiological environment.

1401, 18e Rue
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Québec, Québec
Canada G1J 1Z4
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Duque-Fernandez A, Gauthier L, Simard M, Jean J, Gendreau I, Morin A, Soucy J, Auger M, Pouliot R

A 3D-psoriatic skin model for dermatological testing: The impact of culture conditions.

Journal Article

Biochem Biophys Rep, 8 , pp. 268-276, 2016, ISSN: 2405-5808.

Abstract | Links:

Pare B, Deschenes LT, Pouliot R, Dupre N, Gros-Louis F

An Optimized Approach to Recover Secreted Proteins from Fibroblast Conditioned-Media for Secretomic Analysis.

Journal Article

Front Cell Neurosci, 10 , pp. 70, 2016.

Abstract | Links:

Chabaud S, Simard M, Gendreau I, Pouliot R, Bolduc S

Origin of Serum Affects Quality of Engineered Tissues Produced by the Self-Assembly Approach.

Journal Article

Scientifica (Cairo), 2016 , pp. 3825645, 2016, ISSN: 2090-908X.

Abstract | Links:

Pouliot-Berube C, Zaniolo K, Guerin SL, Pouliot R

Tissue-engineered human psoriatic skin supplemented with cytokines as an in vitro model to study plaque psoriasis.

Journal Article

Regen Med, 11 (6), pp. 545-57, 2016, ISSN: 1746-0751.

Abstract | Links:

Jean J, Leroy M, Duque-Fernandez A, Bernard G, Soucy J, Pouliot R

Characterization of a psoriatic skin model produced with involved or uninvolved cells.

Journal Article

J Tissue Eng Regen Med, 9 (7), pp. 789-98, 2015, ISSN: 1932-6254.

Abstract | Links:

Leroy M, Lefevre T, Pouliot R, Auger M, Laroche G

Using infrared and Raman microspectroscopies to compare ex vivo involved psoriatic skin with normal human skin.

Journal Article

J Biomed Opt, 20 (6), pp. 067004, 2015, ISSN: 1083-3668.

Abstract | Links:

Ayata RE, Chabaud S, Auger M, Pouliot R

Behaviour of endothelial cells in a tridimensional in vitro environment.

Journal Article

Biomed Res Int, 2015 , pp. 630461, 2015.

Abstract | Links:

Ayata RE, Bouhout S, Auger M, Pouliot R

Study of in vitro capillary-like structures in psoriatic skin substitutes.

Journal Article

Biores Open Access, 3 (5), pp. 197-205, 2014, ISSN: 2164-7844.

Abstract | Links:

Leroy M, Labbe JF, Ouellet M, Jean J, Lefevre T, Laroche G, Auger M, Pouliot R

A comparative study between human skin substitutes and normal human skin using Raman microspectroscopy.

Journal Article

Acta Biomater, 10 (6), pp. 2703-11, 2014, ISSN: 1742-7061.

Abstract | Links:

Garcia-Perez ME, Allaeys I, Rusu D, Pouliot R, Janezic TS, Poubelle PE

Picea mariana polyphenolic extract inhibits phlogogenic mediators produced by TNF-α-activated psoriatic keratinocytes: Impact on NF-κB pathway.

Journal Article

J Ethnopharmacol, 151 (1), pp. 265-78, 2014, ISSN: 0378-8741.

Abstract | Links:

48 entries « 2 of 5 »
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Active projects

  • Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
  • Centre de recherche en organogenèse expérimentale, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 2011-05-01 to 2020-09-22
  • Centre Québécois sur les Matériaux Fonctionnels (CQMF), Subvention, Fonds de recherche du Québec - Nature et technologies, Regroupements stratégiques NT, from 2017-04-01 to 2023-03-31
  • Comprendre la complexité du domaine lipidique cutané, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2019-04-01 to 2024-03-31
  • Envisager une protection contre le passage des perturbateurs endocriniens à travers la peau, Subvention, Fondation de l'Université Laval, from 2019-02-01 to 2020-12-31
  • Étude de l'impact de l'innervation cutanée dans le Psoriasis, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2018-04-01 to 2023-03-31
  • Nouvelles options thérapeutiques pour les patients atteints de psoriasis, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2019-10-01 to 2024-09-30

Recently finished projects

  • Centre de recherche en arthrite de l'Université Laval, Subvention, Institutionnel - BDR, BDR - Entités de recherche en émergence, from 2019-01-01 to 2020-01-31
  • Chaire Famille-Louis-Boivin, Subvention, Fondation de l'Université Laval, Chaires de recherche sans organismes subventionnaires, from 1997-04-01 to 2020-03-31
  • Comprendre le rôle des cellules immunitaires dans le psoriasis grâce au génie tissulaire, Subvention, Instituts de recherche en santé du Canada, Subvention de fonctionnement, from 2014-04-01 to 2020-03-31
  • Étude en lipidomique : Comparaison des profils en sphingolipides de peaux saines et psoriasiques, Partenariat, MITACS Inc., Accélération Québec (MITACS/FQRNT), from 2016-01-01 to 2019-06-30
  • Substituted phenyl alkylureas as new potent antipsoriatic drugs: Mechanism of action and structure-activity relationships., Subvention, Instituts de recherche en santé du Canada, Subvention de fonctionnement, from 2015-07-01 to 2020-06-30
Data provided by the Université Laval research projects registery