Trained as a biochemist (UQAM, 1989) and as a molecular and cellular biologist (U. of Montreal 1996 and Karolinska Institutet, Stockholm, Sweden, 2001), Dr. Patrick Provost is a Full Professor in the Department of Microbiology, Infectious diseases and Immunology at Laval University’s School of Medicine and Researcher at the CHU de Québec Research Center/Laval University since 2001. To date, the turning point of his career has been his discovery, in 2002, of the Dicer ribonuclease in humans. This enzyme catalyzes the formation of microRNAs (19 to 24 nucleotides in length), which are now recognized as key regulators of ~ 60% of the genes in humans.

His current research aims to improve our understanding of the molecular mechanisms underlying small non-coding RNA (e.g. microRNAs) biogenesis, function and transfer between cells and species through extracellular vesicles (EVs; 0.1-1 μm in diameter), in the context of human health, nutrition and disease.

His work has been regularly covered by print media (scientific and daily newspapers), given their importance and translational nature.

Release, delivery and cell programming effects of platelet microparticles and microRNAs

Circulating blood platelets contain a large repertoire of microRNAs in the form of Argonaute 2•microRNA effector complexes, which they release through pro-inflammatory Evs, following activation. These microRNAs can thus be transferred to other cells in the circulatory system and induce changes in the programming and function of the recipient cells, at least in part, by heterotypic regulation of the expression of their genes. This project, which allows us to develop new approaches aimed at eliminating platelet EVs from platelet concentrates for transfusion, illustrates the breadth and complexity of intercellular communications in the human body.

Therapeutic applications of milk extracellular vesicles (EVs)

EVs are released from a wide variety of cells into biological fluids, including milk. We found that commercial dairy cow milk contains EVs that are enriched with anti-inflammatory proteins, such as milk fat globule epidermal growth factor 8 (MFG-E8), that may protect their labile cargo of bioactive molecules during digestion. These findings prompted us to explore the therapeutic potential of milk EVs in inflammatory conditions affecting the gastrointestinal tract.

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71 entries « 1 of 15 »

Osman A, Hitzler WE, Provost P

The platelets' perspective to pathogen reduction technologies.

Journal Article

Platelets, 29 (2), pp. 140-147, 2018, ISSN: 0953-7104.

Abstract | Links:

Magron A, Laugier J, Provost P, Boilard E

Pathogen reduction technologies: The pros and cons for platelet transfusion.

Journal Article

Platelets, 29 (1), pp. 2-8, 2018, ISSN: 0953-7104.

Abstract | Links:

Provost P

The clinical significance of platelet microparticle-associated microRNAs.

Journal Article

Clin Chem Lab Med, 55 (5), pp. 657-666, 2017, ISSN: 1434-6621.

Abstract | Links:

Benmoussa A, Ly S, Shan ST, Laugier J, Boilard E, Gilbert C, Provost P

A subset of extracellular vesicles carries the bulk of microRNAs in commercial dairy cow's milk.

Journal Article

J Extracell Vesicles, 6 (1), pp. 1401897, 2017, ISSN: 2001-3078.

Abstract | Links:

Benmoussa A, Lee CH, Laffont B, Savard P, Laugier J, Boilard E, Gilbert C, Fliss I, Provost P

Commercial Dairy Cow Milk microRNAs Resist Digestion under Simulated Gastrointestinal Tract Conditions.

Journal Article

J Nutr, 146 (11), pp. 2206-2215, 2016, ISSN: 0022-3166.

Abstract | Links:

71 entries « 1 of 15 »

Active projects

  • Centre hospitalier universitaire de Québec - CHU de Québec-Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
  • Dietary microRNAs, Subvention, Instituts de recherche en santé du Canada, Subvention de fonctionnement, from 2014-07-01 to 2019-03-31

Recently finished projects

  • Platelet microRNAs during storage under blood bank conditions, Subvention, Instituts de recherche en santé du Canada, Subvention de fonctionnement, from 2013-04-01 to 2016-09-30
  • Release, delivery and cell programming effects of platelet microparticles and microRNAs., Subvention, Instituts de recherche en santé du Canada, Subvention de fonctionnement, from 2014-10-01 to 2018-03-31
  • Role, function and regulation of human Dicer, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2013-04-01 to 2018-03-31
Data provided by the Université Laval research projects registery