Patrick J. Rochette is a regular researcher at the Research Centre of the CHU of Quebec – Laval University, in the area of regenerative medicine, of which he is the assistant director. He is also Associate Professor and Assistant Director of Basic Research in the Department of Ophthalmology and ENT – head and neck surgery of the Faculty of Medicine at Laval University. He completed his graduate studies (MSc / PhD) in the laboratory of Dr. Regen Drouin (U. Laval), where he studied the induction and repair of UV-induced damage. He pursued his training with 2 postdoctoral fellowships at Yale University, including one in the laboratory of Dr. Douglas Brash, a pioneer in the study of UV-induced mutations in cutaneous cancer. His second fellowship took place in Dr. Yilun Liu ‘s laboratory, where he worked on the RecQ4 helicase in the initiation of DNA replication. He joined Laval University in 2009 and started a laboratory where he specialized in photobiology and, more precisely, on the consequences of cell exposure to low-wavelength light rays (ultraviolet and blue). In humans, only the skin and eyes are exposed to these rays. Thus, Dr. Rochette’s laboratory is interested in two fields of study, ocular photobiology and cutaneous photobiology.
At the cutaneous level, skin cells are used as a model to study the mechanisms of protection against the genotoxicity of sunlight. These mechanisms include the induction of mutagenic damage, its repair, and induced cell death as a protective mechanism. In this part of the laboratory, the projects pursued are, among others, the following:
– Influence of the dermis on the efficacy of repair techniques of UV-induced mutagenic damage to the epidermis;
– Effect of chronic UVB irradiation on the cellular genotoxic stress response;
– Necroptosis as a new UV-induced, programmed cell death pathway;
– Involvement of UVA, alone or in synergy with polycyclic aromatic hydrocarbons (PAH) contained in cigarette smoke in cutaneous photoaging.
At the ocular level, Dr. Rochette’s research is mostly applied. It aims to understand the involvement of UV and blue light in various ocular pathologies. As UV light does not reach the adult retina, blue light is the most energetic light it is exposed to. In this part of the laboratory, the projects pursued are, among others, the following:
– UVA toxicity in the cornea, involvement of UV-Induced oxidation;
– Involvement of mitochondrial dysfunction in Fuchs corneal endothelial dystrophy;
– Involvement of blue light, alone or in synergy with lipofuscin and / or PAHs in age-related macular degeneration (AMD).
1050, chemin Sainte-Foy
Canada G1S 4L8
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1050, chemin Sainte-Foy
Canada G1S 4L8
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1050 Chemin Sainte-Foy
Canada G1S 4L8
Bcl-2 is the target of a UV-inducible apoptosis switch and a node for UV signaling.Journal Article
Proc Natl Acad Sci U S A, 104 (27), pp. 11286-91, 2007, ISSN: 0027-8424.
Pyrimidine (6-4) pyrimidone photoproduct mapping after sublethal UVC doses: nucleotide resolution using terminal transferase-dependent PCR.Journal Article
Photochem Photobiol, 82 (5), pp. 1370-6, 2006, ISSN: 0031-8655.
SW480, a p53 double-mutant cell line retains proficiency for some p53 functions.Journal Article
J Mol Biol, 352 (1), pp. 44-57, 2005, ISSN: 0022-2836.
DNA damage induced by UVA radiation : role in solar mutagenesis (Chapter 3)Book Chapter
E, Sage; R, Drouin; M, Rouabhia (Ed.): From DNA Photolesions to Mutations, Skin Cancer and Cell Death, pp. 33-47, Cambridge, Royal Society of Chemistry, 2005, ISBN: 0854043268.
UVA-induced cyclobutane pyrimidine dimers form predominantly at thymine-thymine dipyrimidines and correlate with the mutation spectrum in rodent cells.Journal Article
Nucleic Acids Res, 31 (11), pp. 2786-94, 2003, ISSN: 0305-1048.
Human cells bearing homozygous mutations in the DNA mismatch repair genes hMLH1 or hMSH2 are fully proficient in transcription-coupled nucleotide excision repair.Journal Article
Oncogene, 21 (37), pp. 5743-52, 2002, ISSN: 0950-9232.
- Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
- Centre de recherche en organogenèse expérimentale, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 2011-05-01 to 2020-09-22
- Fuchs endothelial corneal dystrophy and corneal transplantation: The traditional paradigm revisited, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2019-10-01 to 2024-09-30
- Réponse cellulaire au stress génotoxique induit par l'irradiation chronique aux rayons ultraviolets., Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2016-04-01 to 2021-03-31
Recently finished projects
- Toxicité des rayons solaires dans l'oeil humain., Subvention, Fonds de recherche du Québec - Santé, Chercheur-boursier Juniors 1 et 2, Seniors, from 2016-07-01 to 2020-06-30