Pr. Fradette has been a regular researcher at the CRCHU de Québec-Laval University since 2005, and a full professor of the Department of Surgery of the Laval University School of Medicine. After a bachelor’s degree in biochemistry (1991-1994, U Laval) and a PhD in cellular and molecular biology focused on the study of skin epithelial stem cells (1994-1999, U Laval, with L. Germain, LOEX), she acquired a specialization in gene therapy during her postdoctoral fellowship at the University of Pittsburgh (1999-2004, with J. Glorioso III, U Pittsburgh, PA, USA). Her current research program is based on the use of human adipose-derived stromal/stem cells in regenerative medicine. Since September of 2015, she has been the director of the ThéCell network, the province of Quebec network for cell and tissue therapy supported by the FRQS.
Development of human reconstructed tissues as 3D discovery models and for clinical applications
Pr. Fradette’s current research program is based on the use of a new and abundant source of multipotential cells: the adipose-derived stromal/stem cells (ASCs). Using tissue-engineering techniques, her team uses ASCs to recreate human tissue which is needed in reconstructive surgery, such as skin, adipose, and bone-like tissue. For example, human adipose substitutes recreating the 3D tissular context in vitro are useful discovery tools for pharmaco-toxicological studies on the metabolic regulation of human adipocytes. Moreover, adipose tissues are reconstructed with the important goal of being used in the clinic as soft-tissue fillers to repair tissue loss, in cases of deep burns or tumor removal (including mastectomy). The resulting tissues are all human, and devoid of exogenous biomaterials.
Her research interests include a better understanding of the cellular interactions between adipocytes and endothelial cells, the effects of inflammatory conditions on blood capillaries and adipocyte metabolism, the impact of human ECM components on the adipogenic differentiation of ASCs, and how MMPs can influence tissue remodeling, under normal and inflammatory conditions. Current studies performed in her laboratory aim at promoting the vascularisation of thick reconstructed tissues produced under xenogenic-free culture conditions for clinical applications. Ongoing preclinical studies assess the efficacy of tissue-engineered biological dressings to accelerate the healing of skin wounds.
In collaboration with D.r M. Fortin and Dr. FA. Auger, her team also produces bone-like substitutes from ASCs.
Local R-221 LOEX / CMDGT
Canada G1J 1Z4
- Diaz, CandiceMaster studentHôpital de l'Enfant-Jésuscandice.email@example.com@crchudequebec.ulaval.ca
1401 18e Rue
Canada G1J 1Z4
- Gabra, NathalieMaster firstname.lastname@example.org
- Safoine, MeryemDoctoral studentHôpital de l'Enfant-Jésus+1 418-990-8255, extension email@example.com@crchudequebec.ulaval.ca
1401, 18e Rue
Canada G1J 1Z4
- Sorroza Martínez, Luis FernandoMaster studentHôpital de l'Enfant-Jésus+1 418-525-4444, extension firstname.lastname@example.org@crchudequebec.ulaval.ca
1401 18e Rue
Canada G1J 1Z4
- Vézina, JérémieEmployeejeremie.email@example.com
Targeted gene addition to human mesenchymal stromal cells as a cell-based plasma-soluble protein delivery platform.Journal Article
Cytotherapy, 12 (3), pp. 394-9, 2010, ISSN: 1465-3249.
[Porous matrix and primary-cell culture: a shared concept for skin and cornea tissue engineering].Journal Article
Pathol Biol (Paris), 57 (4), pp. 290-8, 2009, ISSN: 0369-8114.
Adipose-tissue engineering: taking advantage of the properties of human adipose-derived stem/stromal cells.Journal Article
Pathol Biol (Paris), 57 (4), pp. 309-17, 2009, ISSN: 0369-8114.
Evolution of three dimensional skin equivalent models reconstructed in vitro by tissue engineering.Journal Article
Eur J Dermatol, 19 (2), pp. 107-13, 2009, ISSN: 1167-1122.
The mixed-lineage kinase DLK is a key regulator of 3T3-L1 adipocyte differentiation.Journal Article
PLoS ONE, 4 (3), pp. e4743, 2009.
Regeneration of skin and cornea by tissue engineering.Journal Article
Methods Mol Biol, 482 , pp. 233-56, 2009, ISSN: 1064-3745.
Human post-natal stem cells in organs produced by tissue engineering for clinical application (Chapter VI)Book Chapter
AV, Faraday; JT, Dyer (Ed.): Progress in Stem Cell Applications, pp. 181-208, New York, NY, Nova Biomedical Books, 2008, ISBN: 978-1-60456-316-0.
- Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
- Centre de recherche en organogenèse expérimentale, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 2011-05-01 to 2020-09-22
- Design and testing of a next-generation tissue-engineered living human heart valve, Subvention, Secrétariat des programmes interorganismes à l’intention des établissements, Nouvelles frontières en recherche, from 2020-03-31 to 2022-03-30
- Réseau de thérapie cellulaire, tissulaire et génique du Québec, Subvention, Fonds de recherche du Québec - Santé, Réseaux thématiques de recherche, from 2019-04-01 to 2023-03-31
- Roles of matricryptins (bioactive extracellular matrix fragments) in adipose tissue, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2020-04-01 to 2025-03-31
- Tissue Engineering of a functional Urethra for Autologous Reconstruction, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2018-04-01 to 2022-03-31
Recently finished projects
- (FRSQ 85117)La guérison des plaies cutanées par utilisation des cellules souches, Subvention, Fondation du CHU de Québec, from 2019-04-09 to 2020-03-31
- Centre de recherche en arthrite de l'Université Laval, Subvention, Institutionnel - BDR, BDR - Entités de recherche en émergence, from 2019-01-01 to 2020-01-31