Pr. Fradette has been a regular researcher at the CRCHU de Québec-Laval University since 2005, and a full professor of the Department of Surgery of the Laval University School of Medicine. After a bachelor’s degree in biochemistry (1991-1994, U Laval) and a PhD in cellular and molecular biology focused on the study of skin epithelial stem cells (1994-1999, U Laval, with L. Germain, LOEX), she acquired a specialization in gene therapy during her postdoctoral fellowship at the University of Pittsburgh (1999-2004, with J. Glorioso III, U Pittsburgh, PA, USA). Her current research program is based on the use of human adipose-derived stromal/stem cells in regenerative medicine. Since September of 2015, she has been the director of the ThéCell network, the province of Quebec network for cell and tissue therapy supported by the FRQS.
Development of human reconstructed tissues as 3D discovery models and for clinical applications
Pr. Fradette’s current research program is based on the use of a new and abundant source of multipotential cells: the adipose-derived stromal/stem cells (ASCs). Using tissue-engineering techniques, her team uses ASCs to recreate human tissue which is needed in reconstructive surgery, such as skin, adipose, and bone-like tissue. For example, human adipose substitutes recreating the 3D tissular context in vitro are useful discovery tools for pharmaco-toxicological studies on the metabolic regulation of human adipocytes. Moreover, adipose tissues are reconstructed with the important goal of being used in the clinic as soft-tissue fillers to repair tissue loss, in cases of deep burns or tumor removal (including mastectomy). The resulting tissues are all human, and devoid of exogenous biomaterials.
Her research interests include a better understanding of the cellular interactions between adipocytes and endothelial cells, the effects of inflammatory conditions on blood capillaries and adipocyte metabolism, the impact of human ECM components on the adipogenic differentiation of ASCs, and how MMPs can influence tissue remodeling, under normal and inflammatory conditions. Current studies performed in her laboratory aim at promoting the vascularisation of thick reconstructed tissues produced under xenogenic-free culture conditions for clinical applications. Ongoing preclinical studies assess the efficacy of tissue-engineered biological dressings to accelerate the healing of skin wounds.
In collaboration with D.r M. Fortin and Dr. FA. Auger, her team also produces bone-like substitutes from ASCs.
Local R-221 LOEX / CMDGT
Canada G1J 1Z4
- Diaz, CandiceMaster studentHôpital de l'Enfant-Jésuscandice.firstname.lastname@example.org@crchudequebec.ulaval.ca
1401 18e Rue
Canada G1J 1Z4
- Gabra, NathalieMaster email@example.com
- Safoine, MeryemDoctoral studentHôpital de l'Enfant-Jésus+1 418-990-8255, extension firstname.lastname@example.org@crchudequebec.ulaval.ca
1401, 18e Rue
Canada G1J 1Z4
- Sorroza Martínez, Luis FernandoMaster studentHôpital de l'Enfant-Jésus+1 418-525-4444, extension email@example.com@crchudequebec.ulaval.ca
1401 18e Rue
Canada G1J 1Z4
- Vézina, JérémieEmployeejeremie.firstname.lastname@example.org
Strategies to promote the vascularization of skin substitutes after transplantation (Chapter 8)Book Chapter
AP, Marques; RP, Pirraco; MT, Cerqueira; RL, Reis (Ed.): Skin Tissue Models, pp. 177-200, London Academic Press, 2018, ISBN: 9780128105450 (paper) / 9780128110003 (eBook).
Corrigendum to "Inflammatory cytokine profiles in visceral and subcutaneous adipose tissues of obese patients undergoing bariatric surgery reveal lack of correlation with obesity or diabetes" EBioMedicine 30C (2018) 237-247.Journal Article
EBioMedicine, 37 , pp. 564-568, 2018, ISSN: 2352-3964.
A Cell-Based Self-Assembly Approach for the Production of Human Osseous Tissues from Adipose-Derived Stromal/Stem Cells.Journal Article
Adv Healthc Mater, 6 (4), 2017, ISSN: 2192-2640.
Role of the TGF-β pathway in dedifferentiation of human mature adipocytes.Journal Article
FEBS Open Bio, 7 (8), pp. 1092-1101, 2017, ISSN: 2211-5463.
Workshop to address gaps in regulation of minimally manipulated autologous cell therapies for homologous use in Canada.Journal Article
Cytotherapy, 19 (12), pp. 1400-1411, 2017, ISSN: 1465-3249.
Deficiency of Interleukin-15 Confers Resistance to Obesity by Diminishing Inflammation and Enhancing the Thermogenic Function of Adipose Tissues.Journal Article
PLoS ONE, 11 (9), pp. e0162995, 2016.
Correction: Deficiency of Interleukin-15 Confers Resistance to Obesity by Diminishing Inflammation and Enhancing the Thermogenic Function of Adipose Tissues.Journal Article
PLoS ONE, 11 (11), pp. e0166537, 2016.
Impact of TNF and IL-1b on capillary networks within engineered human adipose tissuesJournal Article
J Mater Chem B Mater Biol Med, 4 , pp. 3608-3619, 2016, ISSN: 2050-750X.
Adipose-derived stromal cells for the reconstruction of a human vesical equivalent.Journal Article
J Tissue Eng Regen Med, 9 (11), pp. E135-43, 2015, ISSN: 1932-6254.
Oxidative activity of 17β-hydroxysteroid dehydrogenase on testosterone in male abdominal adipose tissues and cellular localization of 17β-HSD type 2.Journal Article
Mol Cell Endocrinol, 414 , pp. 168-76, 2015, ISSN: 0303-7207.
- Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
- Centre de recherche en organogenèse expérimentale, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 2011-05-01 to 2020-09-22
- Design and testing of a next-generation tissue-engineered living human heart valve, Subvention, Secrétariat des programmes interorganismes à l’intention des établissements, Nouvelles frontières en recherche, from 2020-03-31 to 2022-03-30
- Réseau de thérapie cellulaire, tissulaire et génique du Québec, Subvention, Fonds de recherche du Québec - Santé, Réseaux thématiques de recherche, from 2019-04-01 to 2023-03-31
- Roles of matricryptins (bioactive extracellular matrix fragments) in adipose tissue, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2020-04-01 to 2025-03-31
- Tissue Engineering of a functional Urethra for Autologous Reconstruction, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2018-04-01 to 2022-03-31
Recently finished projects
- (FRSQ 85117)La guérison des plaies cutanées par utilisation des cellules souches, Subvention, Fondation du CHU de Québec, from 2019-04-09 to 2020-03-31
- Centre de recherche en arthrite de l'Université Laval, Subvention, Institutionnel - BDR, BDR - Entités de recherche en émergence, from 2019-01-01 to 2020-01-31