Pr. Fradette has been a regular researcher at the CRCHU de Québec-Laval University since 2005, and a full professor of the Department of Surgery of the Laval University School of Medicine. After a bachelor’s degree in biochemistry (1991-1994, U Laval) and a PhD in cellular and molecular biology focused on the study of skin epithelial stem cells (1994-1999, U Laval, with L. Germain, LOEX), she acquired a specialization in gene therapy during her postdoctoral fellowship at the University of Pittsburgh (1999-2004, with J. Glorioso III, U Pittsburgh, PA, USA). Her current research program is based on the use of human adipose-derived stromal/stem cells in regenerative medicine. Since September of 2015, she has been the director of the ThéCell network, the province of Quebec network for cell and tissue therapy supported by the FRQS.
Development of human reconstructed tissues as 3D discovery models and for clinical applications
Pr. Fradette’s current research program is based on the use of a new and abundant source of multipotential cells: the adipose-derived stromal/stem cells (ASCs). Using tissue-engineering techniques, her team uses ASCs to recreate human tissue which is needed in reconstructive surgery, such as skin, adipose, and bone-like tissue. For example, human adipose substitutes recreating the 3D tissular context in vitro are useful discovery tools for pharmaco-toxicological studies on the metabolic regulation of human adipocytes. Moreover, adipose tissues are reconstructed with the important goal of being used in the clinic as soft-tissue fillers to repair tissue loss, in cases of deep burns or tumor removal (including mastectomy). The resulting tissues are all human, and devoid of exogenous biomaterials.
Her research interests include a better understanding of the cellular interactions between adipocytes and endothelial cells, the effects of inflammatory conditions on blood capillaries and adipocyte metabolism, the impact of human ECM components on the adipogenic differentiation of ASCs, and how MMPs can influence tissue remodeling, under normal and inflammatory conditions. Current studies performed in her laboratory aim at promoting the vascularisation of thick reconstructed tissues produced under xenogenic-free culture conditions for clinical applications. Ongoing preclinical studies assess the efficacy of tissue-engineered biological dressings to accelerate the healing of skin wounds.
In collaboration with D.r M. Fortin and Dr. FA. Auger, her team also produces bone-like substitutes from ASCs.
Local R-221 LOEX / CMDGT
Canada G1J 1Z4
- Diaz, CandiceMaster studentHôpital de l'Enfant-Jésuscandice.firstname.lastname@example.org@crchudequebec.ulaval.ca
1401 18e Rue
Canada G1J 1Z4
- Gabra, NathalieMaster email@example.com
- Safoine, MeryemDoctoral studentHôpital de l'Enfant-Jésus+1 418-990-8255, extension firstname.lastname@example.org@crchudequebec.ulaval.ca
1401, 18e Rue
Canada G1J 1Z4
- Sorroza Martínez, Luis FernandoMaster studentHôpital de l'Enfant-Jésus+1 418-525-4444, extension email@example.com@crchudequebec.ulaval.ca
1401 18e Rue
Canada G1J 1Z4
- Vézina, JérémieEmployeejeremie.firstname.lastname@example.org
Adipose-derived stromal cells for the reconstruction of a human vesical equivalent.Journal Article
J Tissue Eng Regen Med, 9 (11), pp. E135-43, 2015, ISSN: 1932-6254.
Oxidative activity of 17β-hydroxysteroid dehydrogenase on testosterone in male abdominal adipose tissues and cellular localization of 17β-HSD type 2.Journal Article
Mol Cell Endocrinol, 414 , pp. 168-76, 2015, ISSN: 0303-7207.
Human adipose-derived stromal cells for the production of completely autologous self-assembled tissue-engineered vascular substitutes.Journal Article
Acta Biomater, 24 , pp. 209-19, 2015, ISSN: 1742-7061.
Enhancing repair of full-thickness excisional wounds in a murine model: Impact of tissue-engineered biological dressings featuring human differentiated adipocytes.Journal Article
Acta Biomater, 22 , pp. 39-49, 2015, ISSN: 1742-7061.
Magnetic Resonance Imaging of Human Tissue-Engineered Adipose Substitutes.Journal Article
Tissue Eng Part C Methods, 21 (7), pp. 693-704, 2015, ISSN: 1937-3384.
Melatonin pretreatment of human adipose tissue-derived mesenchymal stromal cells enhances their prosurvival and protective effects on human kidney cells.Journal Article
Am J Physiol Renal Physiol, 308 (12), pp. F1474-83, 2015, ISSN: 0363-6127.
Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells.Journal Article
Stem Cell Reports, 4 (6), pp. 1016-30, 2015, ISSN: 2213-6711.
Creating capillary networks within human engineered tissues: impact of adipocytes and their secretory products.Journal Article
Acta Biomater, 11 , pp. 333-45, 2015, ISSN: 1742-7061.
Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-α.Journal Article
PLoS ONE, 10 (9), pp. e0137612, 2015.
Thérapie cellulaire et génique (Chapitre 7)Book Chapter
P, Beaulieu; V, Pichette; J, Desroches; Souich, Du (Ed.): Précis de pharmacologie : du fondamental à la clinique, pp. 161-182, Montréal, QC, Les Presses de l'Université de Montréal, 2015, ISBN: 9782760634527.
- Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
- Centre de recherche en organogenèse expérimentale, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 2011-05-01 to 2020-09-22
- Tissue Engineering of a functional Urethra for Autologous Reconstruction, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2018-04-01 to 2022-03-31
Recently finished projects
- (FRSQ 81041)«Analyse de la guérison de plaies cutanées induites par la radiation», Subvention, Fondation du CHU de Québec, from 2018-03-14 to 2019-03-13
- Centre de recherche en arthrite de l'Université Laval, Subvention, Institutionnel - BDR, BDR - Entités de recherche en émergence, from 2019-01-01 to 2020-01-31
- Elucidation of the functional rôle of Complement Factor D in Acute Myeloid Leukemia, Subvention, Société de leucémie et lymphome du Canada, from 2017-07-01 to 2018-06-30
- Stratégies de préconditionnement tissulaire afin de mitiger les effets de l’inflammation post-implantation et augmenter le succès des greffes de tissus adipeux, Subvention, Fondation de l'Université Laval, from 2016-09-15 to 2018-09-14