Dr. Lambert is a regular researcher at the CHU de Québec-Université Laval Research Center and an assistant professor at the Faculty of Medicine of l’Université Laval in the department of Molecular Medicine. Dr. Lambert’s work aims to decipher how epigenetic regulators are assembled into functional units at the level of chromatin and how they impact transcription in healthy and cancerous cells. To address these fundamental questions, Dr. Lambert employs a multidisciplinary approach that includes mass spectrometry-based proteomics, protein biochemistry and cancer biology. He was recently awarded a prestigious Scholarship for the Next Generation of Scientists from the Cancer Research Society to support him as he establishes his research program.

Characterization of bromodomain-containing proteins in hormone-responsive cancers

Bromodomain-containing proteins are epigenetic regulators involved in many facets of the transcription process. Recently, numerous bromodomain inhibitors have been developed enabling the modulation of their activity for therapeutic benefit. Dr. Lambert’s group is currently investigating the functions of bromodomain-containing proteins in the context of hormone-responsive breast cancers to define their physical interplay with hormone receptors. In addition, Dr. Lambert is defining how specific cancer contexts impinged on bromodomain-containing proteins function and whether they generate actionable vulnerabilities that can targeted with bromodomain inhibitors.

Development of innovative functional proteomics technologies

To enable functional studies of epigenetic regulators, Dr. Lambert’s group is developing and implementing novel proteomics technologies to enable the study of proteomes, protein-protein interactions and post-translational modifications from clinically relevant samples. A particular focus of Dr. Lambert’s efforts remains the study of protein complexes associated with chromatin allowing the elucidation of the mechanisms enabling the ordered recruitment of epigenetic regulators to particular genomic loci.

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62 entries « 3 of 7 »

Billon P, Li J, Lambert JP, Chen Y, Tremblay V, Brunzelle JS, Gingras AC, Verreault A, Sugiyama T, Couture JF, Cote J

Acetylation of PCNA Sliding Surface by Eco1 Promotes Genome Stability through Homologous Recombination.

Journal Article

Mol Cell, 65 (1), pp. 78-90, 2017, ISSN: 1097-2765.

Abstract | Links:

Savitsky P, Krojer T, Fujisawa T, Lambert JP, Picaud S, Wang CY, Shanle EK, Krajewski K, Friedrichsen H, Kanapin A, Goding C, Schapira M, Samsonova A, Strahl BD, Gingras AC, Filippakopoulos P

Multivalent Histone and DNA Engagement by a PHD/BRD/PWWP Triple Reader Cassette Recruits ZMYND8 to K14ac-Rich Chromatin.

Journal Article

Cell Rep, 17 (10), pp. 2724-2737, 2016.

Abstract | Links:

Liu G, Knight JD, Zhang JP, Tsou CC, Wang J, Lambert JP, Larsen B, Tyers M, Raught B, Bandeira N, Nesvizhskii AI, Choi H, Gingras AC

Data Independent Acquisition analysis in ProHits 4.0.

Journal Article

J Proteomics, 149 , pp. 64-68, 2016, ISSN: 1874-3919.

Abstract | Links:

Picaud S, Leonards K, Lambert JP, Dovey O, Wells C, Fedorov O, Monteiro O, Fujisawa T, Wang CY, Lingard H, Tallant C, Nikbin N, Guetzoyan L, Ingham R, Ley SV, Brennan P, Muller S, Samsonova A, Gingras AC, Schwaller J, Vassiliou G, Knapp S, Filippakopoulos P

Promiscuous targeting of bromodomains by bromosporine identifies BET proteins as master regulators of primary transcription response in leukemia.

Journal Article

Sci Adv, 2 (10), pp. e1600760, 2016, ISSN: 2375-2548.

Abstract | Links:

Jacquet K, Fradet-Turcotte A, Avvakumov N, Lambert JP, Roques C, Pandita RK, Paquet E, Herst P, Gingras AC, Pandita TK, Legube G, Doyon Y, Durocher D, Cote J

The TIP60 Complex Regulates Bivalent Chromatin Recognition by 53BP1 through Direct H4K20me Binding and H2AK15 Acetylation.

Journal Article

Mol Cell, 62 (3), pp. 409-421, 2016, ISSN: 1097-2765.

Abstract | Links:

Feng Y, Vlassis A, Roques C, Lalonde ME, Gonzalez-Aguilera C, Lambert JP, Lee SB, Zhao X, Alabert C, Johansen JV, Paquet E, Yang XJ, Gingras AC, Cote J, Groth A

BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation.

Journal Article

EMBO J, 35 (2), pp. 176-92, 2016, ISSN: 0261-4189.

Abstract | Links:

Shekhar-Guturja T, Gunaherath GM, Wijeratne EM, Lambert JP, Averette AF, Lee SC, Kim T, Bahn YS, Tripodi F, Ammar R, Dohl K, Niewola-Staszkowska K, Schmitt L, Loewith RJ, Roth FP, Sanglard D, Andes D, Nislow C, Coccetti P, Gingras AC, Heitman J, Gunatilaka AA, Cowen LE

Dual action antifungal small molecule modulates multidrug efflux and TOR signaling.

Journal Article

Nat Chem Biol, 12 (10), pp. 867-75, 2016, ISSN: 1552-4450.

Abstract | Links:

Teo G, Koh H, Fermin D, Lambert JP, Knight JD, Gingras AC, Choi H

SAINTq: Scoring protein-protein interactions in affinity purification - mass spectrometry experiments with fragment or peptide intensity data.

Journal Article

Proteomics, 16 (15-16), pp. 2238-45, 2016, ISSN: 1615-9853.

Abstract | Links:

Wang J, Tucholska M, Knight JD, Lambert JP, Tate S, Larsen B, Gingras AC, Bandeira N

MSPLIT-DIA: sensitive peptide identification for data-independent acquisition.

Journal Article

Nat Methods, 12 (12), pp. 1106-8, 2015, ISSN: 1548-7091.

| Links:

Lambert JP, Tucholska M, Go C, Knight JD, Gingras AC

Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes.

Journal Article

J Proteomics, 118 , pp. 81-94, 2015, ISSN: 1874-3919.

Abstract | Links:

62 entries « 3 of 7 »
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Active projects

  • Caractérisation fonctionnelle des régulateurs épigénétiques et de leurs rôles dans des modèles de cancer, Subvention, Fonds de recherche du Québec - Santé, Chercheur-boursier Juniors 1 et 2, Seniors, from 2018-07-01 to 2022-06-30
  • Caractérisation fonctionnelle des régulateurs épigénétiques et de leurs rôles dans des modèles de cancer, Subvention, Fonds de recherche du Québec - Santé, Établissement de jeunes chercheurs - Juniors 1, from 2018-07-01 to 2022-06-30
  • Centre de recherche sur le cancer, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 1996-05-01 to 2022-06-13
  • Characterization of the scaffolding roles of bromodomain containing proteins at the level of chromatin, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2017-04-01 to 2022-03-31
  • Elucidation of bromodomain functions within SWI/SNF complexes (Prix), Subvention, Instituts de recherche en santé du Canada, Subvention Projet - Annonce de priorités : Prix : Chercheur en début de carrière dans le domaine du cancer, from 2020-03-01 to 2021-02-28
  • Elucidation of bromodomain functions within SWI/SNF complexes, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2020-04-01 to 2025-03-31
  • Establishment of an infrastructure for functional proteomics studies of cancer, Partenariat, Fondation Canadienne pour l'innovation (La), Fonds des leaders John-R.-Evans (FLJR), from 2018-09-01 to 2021-06-30
  • Using BET bromodomain inhibitors to create phenotypic lethality in melanoma, Subvention, Société de recherche sur le cancer, Subvention de fonctionnement, from 2020-09-01 to 2022-08-31

Recently finished projects

  • Bromodomain and extra-terminal (BET) proteins, novel therapeutic targets in melanoma, Subvention, Société de recherche sur le cancer, Bourse pour la relève scientifique, from 2017-07-01 to 2019-06-30
  • High throughput analysis of protein interactions and modifications using peptide arrays, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions d'outils et d'instruments de recherche (OIR), from 2019-03-28 to 2020-03-31
  • Programme de soutien aux nouveaux professeurs de la Faculté de médecine, Subvention, Université Laval - démarrage nouveau chercheur, Soutien facultaire aux jeunes chercheurs de la Faculté de médecine, from 2017-07-01 to 2019-06-30
Data provided by the Université Laval research projects registery