Dr. Lambert is a regular researcher at the CHU de Québec-Université Laval Research Center and an assistant professor at the Faculty of Medicine of l’Université Laval in the department of Molecular Medicine. Dr. Lambert’s work aims to decipher how epigenetic regulators are assembled into functional units at the level of chromatin and how they impact transcription in healthy and cancerous cells. To address these fundamental questions, Dr. Lambert employs a multidisciplinary approach that includes mass spectrometry-based proteomics, protein biochemistry and cancer biology. He was recently awarded a prestigious Scholarship for the Next Generation of Scientists from the Cancer Research Society to support him as he establishes his research program.

Characterization of bromodomain-containing proteins in hormone-responsive cancers

Bromodomain-containing proteins are epigenetic regulators involved in many facets of the transcription process. Recently, numerous bromodomain inhibitors have been developed enabling the modulation of their activity for therapeutic benefit. Dr. Lambert’s group is currently investigating the functions of bromodomain-containing proteins in the context of hormone-responsive breast cancers to define their physical interplay with hormone receptors. In addition, Dr. Lambert is defining how specific cancer contexts impinged on bromodomain-containing proteins function and whether they generate actionable vulnerabilities that can targeted with bromodomain inhibitors.

Development of innovative functional proteomics technologies

To enable functional studies of epigenetic regulators, Dr. Lambert’s group is developing and implementing novel proteomics technologies to enable the study of proteomes, protein-protein interactions and post-translational modifications from clinically relevant samples. A particular focus of Dr. Lambert’s efforts remains the study of protein complexes associated with chromatin allowing the elucidation of the mechanisms enabling the ordered recruitment of epigenetic regulators to particular genomic loci.

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66 entries « 1 of 7 »

Nabeel-Shah S, Garg J, Kougnassoukou Tchara PE, Pearlman RE, Lambert JP, Fillingham J

Functional proteomics protocol for the identification of interaction partners in .

Journal Article

STAR Protoc, 2 (1), 2021.

Abstract | Links:

Dionne U, Bourgault E, Dube AK, Bradley D, Chartier FJM, Dandage R, Dibyachintan S, Despres PC, Gish GD, Pham NTH, Letourneau M, Lambert JP, Doucet N, Bisson N, Landry CR

Protein context shapes the specificity of SH3 domain-mediated interactions in vivo.

Journal Article

Nat Commun, 12 (1), 2021.

Abstract | Links:

Nabeel-Shah S, Garg J, Saettone A, Ashraf K, Lee H, Wahab S, Ahmed N, Fine J, Derynck J, Pu S, Ponce M, Marcon E, Zhang Z, Greenblatt JF, Pearlman RE, Lambert JP, Fillingham J

Functional characterization of RebL1 highlights the evolutionary conservation of oncogenic activities of the RBBP4/7 orthologue in Tetrahymena thermophila.

Journal Article

Nucleic Acids Res, 49 (11), 2021.

Abstract | Links:

Go CD, Knight JDR, Rajasekharan A, Rathod B, Abe KT, Youn JY, Samavarchi-Tehrani P, Zhang H, Popiel E, Lambert JP, Coyaud É, Rajendran D, Wong CJ, Shoubridge EA, Raught B, Hesketh GG, Zhu LY, Lambert JP, Cheung SWT, Antonicka H, Pelletier L, Palazzo AF, Gingras AC

A proximity-dependent biotinylation map of a human cell.

Journal Article

Nature, 595 (7865), 2021.

Abstract | Links:

Kothari C, Osseni MA, Agbo L, Ouellette G, Deraspe M, Laviolette F, Corbeil J, Lambert JP, Diorio C, Durocher F

Machine learning analysis identifies genes differentiating triple negative breast cancers.

Journal Article

Sci Rep, 10 (1), 2020.

Abstract | Links:

Nabeel-Shah S, Ashraf K, Saettone A, Garg J, Derynck J, Lambert JP, Pearlman RE, Fillingham J

Nucleus-specific linker histones Hho1 and Mlh1 form distinct protein interactions during growth, starvation and development in Tetrahymena thermophila.

Journal Article

Sci Rep, 10 (1), 2020.

Abstract | Links:

Fages J, Chailleux C, Humbert J, Jang SM, Loehr J, Lambert JP, Cote J, Trouche D, Canitrot Y

JMJD6 participates in the maintenance of ribosomal DNA integrity in response to DNA damage.

Journal Article

PLoS Genet, 16 (6), 2020.

Abstract | Links:

Louphrasitthiphol P, Siddaway R, Loffreda A, Pogenberg V, Friedrichsen H, Schepsky A, Zeng Z, Lu M, Strub T, Freter R, Lisle R, Suer E, Thomas B, Schuster-Bockler B, Filippakopoulos P, Middleton M, Lu X, Patton EE, Davidson I, Lambert JP, Wilmanns M, Steingrimsson E, Mazza D, Goding CR

Tuning Transcription Factor Availability through Acetylation-Mediated Genomic Redistribution.

Journal Article

Mol Cell, 79 (3), 2020.

Abstract | Links:

Kougnassoukou Tchara PE, Filippakopoulos P, Lambert JP

Emerging tools to investigate bromodomain functions.

Journal Article

Methods, 184 , 2020.

Abstract | Links:

Maltais R, Ngueta Djiemeny A, Roy J, Barbeau X, Lambert JP, Poirier D

Design and synthesis of dansyl-labeled inhibitors of steroid sulfatase for optical imaging.

Journal Article

Bioorg Med Chem, 28 (7), 2020.

Abstract | Links:

66 entries « 1 of 7 »
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Active projects

  • Caractérisation fonctionnelle des régulateurs épigénétiques et de leurs rôles dans des modèles de cancer, from 2018-07-01 to 2022-06-30
  • Caractérisation fonctionnelle des régulateurs épigénétiques et de leurs rôles dans des modèles de cancer, from 2018-07-01 to 2022-06-30
  • Characterization of the scaffolding roles of bromodomain containing proteins at the level of chromatin, from 2017-04-01 to 2022-03-31
  • Covid-19 effects on ARTErial StIffness and vascular AgiNg (CARTESIAN) study- Canada, from 2021-06-01 to 2022-05-31
  • COVID19 persistent symptomatology: an investigation of the metabolomic and proteomic underpinning, from 2021-06-01 to 2022-05-31
  • Elucidation of bromodomain functions within SWI/SNF complexes, from 2020-04-01 to 2025-03-31
  • Predict to prevent: Advanced proteomics profiling for precision medicine, from 2021-03-23 to 2022-09-30
  • Using BET bromodomain inhibitors to create phenotypic lethality in melanoma, from 2020-09-01 to 2022-08-31

Recently finished projects

  • Elucidation of bromodomain functions within SWI/SNF complexes (Prix), from 2020-03-01 to 2021-02-28
  • Establishment of an infrastructure for functional proteomics studies of cancer, from 2018-09-01 to 2021-06-30
  • High throughput analysis of protein interactions and modifications using peptide arrays, from 2019-03-28 to 2020-03-31
Data provided by the Université Laval research projects registery