Dr. Lambert is a regular researcher at the CHU de Québec-Université Laval Research Center and an assistant professor at the Faculty of Medicine of l’Université Laval in the department of Molecular Medicine. Dr. Lambert’s work aims to decipher how epigenetic regulators are assembled into functional units at the level of chromatin and how they impact transcription in healthy and cancerous cells. To address these fundamental questions, Dr. Lambert employs a multidisciplinary approach that includes mass spectrometry-based proteomics, protein biochemistry and cancer biology. He was recently awarded a prestigious Scholarship for the Next Generation of Scientists from the Cancer Research Society to support him as he establishes his research program.

Characterization of bromodomain-containing proteins in hormone-responsive cancers

Bromodomain-containing proteins are epigenetic regulators involved in many facets of the transcription process. Recently, numerous bromodomain inhibitors have been developed enabling the modulation of their activity for therapeutic benefit. Dr. Lambert’s group is currently investigating the functions of bromodomain-containing proteins in the context of hormone-responsive breast cancers to define their physical interplay with hormone receptors. In addition, Dr. Lambert is defining how specific cancer contexts impinged on bromodomain-containing proteins function and whether they generate actionable vulnerabilities that can targeted with bromodomain inhibitors.

Development of innovative functional proteomics technologies

To enable functional studies of epigenetic regulators, Dr. Lambert’s group is developing and implementing novel proteomics technologies to enable the study of proteomes, protein-protein interactions and post-translational modifications from clinically relevant samples. A particular focus of Dr. Lambert’s efforts remains the study of protein complexes associated with chromatin allowing the elucidation of the mechanisms enabling the ordered recruitment of epigenetic regulators to particular genomic loci.

CHUL
2705, boulevard Laurier
R-4720
Québec, QC
Canada G1V 4G2
56 entries « 1 of 6 »

Kougnassoukou Tchara PE, Filippakopoulos P, Lambert JP

Emerging tools to investigate bromodomain functions.

Journal Article

Methods, 2019, ISSN: 1046-2023.

Abstract | Links:

Agbo L, Lambert JP

Proteomics contribution to the elucidation of the steroid hormone receptors functions.

Journal Article

J Steroid Biochem Mol Biol, 192 , pp. 105387, 2019, ISSN: 0960-0760.

Abstract | Links:

Garg J, Saettone A, Nabeel-Shah S, Cadorin M, Ponce M, Marquez S, Pu S, Greenblatt J, Lambert JP, Pearlman RE, Fillingham J

The Med31 Conserved Component of the Divergent Mediator Complex in Tetrahymena thermophila Participates in Developmental Regulation.

Journal Article

Curr Biol, 29 (14), pp. 2371-2379.e6, 2019, ISSN: 0960-9822.

Abstract | Links:

Ashraf K, Nabeel-Shah S, Garg J, Saettone A, Derynck J, Gingras AC, Lambert JP, Pearlman RE, Fillingham J

Proteomic Analysis of Histones H2A/H2B and Variant Hv1 in Tetrahymena thermophila Reveals an Ancient Network of Chaperones.

Journal Article

Mol Biol Evol, 36 (5), pp. 1037-1055, 2019, ISSN: 0737-4038.

Abstract | Links:

Saettone A, Nabeel-Shah S, Garg J, Lambert JP, Pearlman RE, Fillingham J

Functional Proteomics of Nuclear Proteins in Tetrahymena thermophila: A Review.

Journal Article

Genes (Basel), 10 (5), 2019.

Abstract | Links:

Herbert K, Binet R, Lambert JP, Louphrasitthiphol P, Kalkavan H, Sesma-Sanz L, Robles-Espinoza CD, Sarkar S, Suer E, Andrews S, Chauhan J, Roberts ND, Middleton MR, Gingras AC, Masson JY, Larue L, Falletta P, Goding CR

BRN2 suppresses apoptosis, reprograms DNA damage repair, and is associated with a high somatic mutation burden in melanoma.

Journal Article

Genes Dev, 33 (5-6), pp. 310-332, 2019, ISSN: 0890-9369.

Abstract | Links:

Lambert JP, Picaud S, Fujisawa T, Hou H, Savitsky P, Uuskula-Reimand L, Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC

Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains.

Journal Article

Mol Cell, 73 (3), pp. 621-638.e17, 2019, ISSN: 1097-2765.

Abstract | Links:

Lashgari A, Lambert JP, Cote J

Measurement and Analysis of Lysine Acetylation by KAT Complexes In Vitro and In Vivo.

Journal Article

Methods Mol Biol, 1983 , pp. 57-77, 2019, ISSN: 1064-3745.

Abstract | Links:

Banerjee SL, Dionne U, Lambert JP, Bisson N

Targeted proteomics analyses of phosphorylation-dependent signalling networks.

Journal Article

J Proteomics, 189 , pp. 39-47, 2018, ISSN: 1874-3919.

Abstract | Links:

Saettone A, Garg J, Lambert JP, Nabeel-Shah S, Ponce M, Burtch A, Thuppu Mudalige C, Gingras AC, Pearlman RE, Fillingham J

The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila.

Journal Article

Epigenetics Chromatin, 11 (1), pp. 10, 2018.

Abstract | Links:

56 entries « 1 of 6 »
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Active projects

  • Caractérisation fonctionnelle des régulateurs épigénétiques et de leurs rôles dans des modèles de cancer, Subvention, Fonds de recherche du Québec - Santé, Chercheur-boursier Juniors 1 et 2, Seniors, from 2018-07-01 to 2022-06-30
  • Caractérisation fonctionnelle des régulateurs épigénétiques et de leurs rôles dans des modèles de cancer, Subvention, Fonds de recherche du Québec - Santé, Établissement de jeunes chercheurs - Juniors 1, from 2018-07-01 to 2022-06-30
  • Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
  • Centre de recherche sur le cancer, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 1996-05-01 to 2023-04-30
  • Characterization of the scaffolding roles of bromodomain containing proteins at the level of chromatin, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2017-04-01 to 2022-03-31
  • Establishment of an infrastructure for functional proteomics studies of cancer, Partenariat, Fondation Canadienne pour l'innovation (La), Fonds des leaders John-R.-Evans (FLJR), from 2018-09-01 to 2019-12-31
  • High throughput analysis of protein interactions and modifications using peptide arrays, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions d'outils et d'instruments de recherche (OIR), from 2019-03-28 to 2020-03-31

Recently finished projects

  • Bromodomain and extra-terminal (BET) proteins, novel therapeutic targets in melanoma, Subvention, Société de recherche sur le cancer, Bourse pour la relève scientifique, from 2017-07-01 to 2019-06-30
  • Chercheur boursier - Complément de salaire, Subvention, Université Laval - budget de fonctionnement, Compléments de salaire des professeurs sous octroi, from 2017-05-01 to 2018-04-30
  • Programme de soutien aux nouveaux professeurs de la Faculté de médecine, Subvention, Université Laval - démarrage nouveau chercheur, Soutien facultaire aux jeunes chercheurs de la Faculté de médecine, from 2017-07-01 to 2019-06-30
Data provided by the Université Laval research projects registery