Dr. François Berthod is a professor at the Department of Surgery of the Laval University School of Medicine, and researcher at LOEX, Hôpital de l’Enfant-Jésus, CHU de Québec-Laval University research center.

The main objective of his research program is to use tissue-engineering techniques of in vitro organ reconstruction to model different diseases that can be induced, or modulated by the nervous system. These models will be useful to better understand the disease mechanisms and to find new therapeutic approaches. In addition, one of his projects aims to repair peripheral nerve injuries through the development of a nerve tube produced by tissue engineering with the patient’s own cells.

Development of in vitro models of diseases by tissue engineering

Many human diseases have causes and mechanisms that are still poorly understood, making it difficult to find more efficient drugs. Using patient cells, it is possible to reconstruct organs affected by these diseases, and recapitulate their effects in vitro to better understand their mechanisms, and use these models to test the efficacy of new drugs.

In the case of modeling psoriasis, Dr. Berthod’s team hypothesized that cutaneous innervation could play a major role in lesion formation, and developed, in collaboration with Dr. Roxane Pouliot, an innervated and immunocompetent psoriatic skin model to study this disease.

To model amyotrophic lateral sclerosis, a neurodegenerative disease, Dr. Berthod’s team uses tissue engineering tools. Motor neurons are cultured with astrocytes, microglia, Schwann cells, and myotubes in a compartmentalized three-dimensional construct to recapitulate the neurodegenerative process.

Repair of peripheral nerves by tissue engineering

Peripheral nerve injuries cause paralysis of the affected limbs. The current surgical options for repairing major deficits are ineffective. Dr. Berthod has developed, in collaboration with Dr. Hélène Khuong, neurosurgeon, a project to produce a nerve tube made up of the patient’s own cells, and tailored to bridge the nerve gap. The production of an autologous living tube is a major innovation that should greatly stimulate axonal migration in the graft and promote regeneration of the injured nerve, allowing motor and sensitive recoveries of the limb.

1401, 18e Rue
Local: R-205 - LOEX / CMDGT
Québec, Québec
Canada G1J 1Z4
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Hudon V, Berthod F, Black AF, Damour O, Germain L, Auger FA

A tissue-engineered endothelialized dermis to study the modulation of angiogenic and angiostatic molecules on capillary-like tube formation in vitro.

Journal Article

Br J Dermatol, 148 (6), pp. 1094-104, 2003, ISSN: 0007-0963.

Abstract | Links:

Germain L, Berthod F, Moulin V, Goulet F, Auger FA

Principles of Living Organs Reconstruction by Tissue Engineering (Chapter 10)

Book Chapter

MJ, Yaszemski; DJ, Trantolo; KU, Lewandrowski; V, Hasirci; DE, Altobelli; DL, Wise (Ed.): Tissue Engineering and Novel Delivery Systems, pp. 246-286, Boca Raton, FL, CRC Press, 2003, ISBN: 0-8247-4786-0 (Print) / 9780203913338 (eBook).

Germain L, Goulet F, Moulin V, Berthod F, Auger FA

Engineering human tissues for in vivo applications.

Journal Article

Ann N Y Acad Sci, 961 , pp. 268-70, 2002, ISSN: 0077-8923.

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Berthod F, Germain L, Li H, Xu W, Damour O, Auger FA

Collagen fibril network and elastic system remodeling in a reconstructed skin transplanted on nude mice.

Journal Article

Matrix Biol, 20 (7), pp. 463-73, 2001, ISSN: 0945-053X.

Abstract | Links:

Germain L, Moulin V, Berthod F, Carlos A, López Valle CA, Goulet F, Auger FA

Multiple applications of tissue-engineered human skin

Book Chapter

RE, Horch; AM, Munster; BM, Achauer (Ed.): Cultured human keratinocytes and tissue engineered skin substitutes, pp. 91-, Stutgart, Georg Thieme, 2001, ISBN: 9783131301611.

Auger FA, Berthod F, Goulet F, Germain L

What is new in mechanical properties of tissue-engineered organs.

Journal Article

Curr Top Pathol, 93 , pp. 87-93, 1999, ISSN: 0070-2188.

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Auger FA, Rouabhia M, Goulet F, Berthod F, Moulin V, Germain L

Tissue-engineered human skin substitutes developed from collagen-populated hydrated gels: clinical and fundamental applications.

Journal Article

Med Biol Eng Comput, 36 (6), pp. 801-12, 1998, ISSN: 0140-0118.

Abstract | Links:

Black AF, Berthod F, L'heureux N, Germain L, Auger FA

In vitro reconstruction of a human capillary-like network in a tissue-engineered skin equivalent.

Journal Article

FASEB J, 12 (13), pp. 1331-40, 1998, ISSN: 0892-6638.

Abstract | Links:

Li H, Berthod F, Xu W, Damour O, Germain L, Auger FA

Use of in vitro reconstructed skin To cover skin flap donor site.

Journal Article

J Surg Res, 73 (2), pp. 143-8, 1997, ISSN: 0022-4804.

Abstract | Links:

Berthod F, Germain L, Guignard R, Lethias C, Garrone R, Damour O, van der Rest M, Auger FA

Differential expression of collagens XII and XIV in human skin and in reconstructed skin.

Journal Article

J Invest Dermatol, 108 (5), pp. 737-42, 1997, ISSN: 0022-202X.

Abstract | Links:

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Active projects

  • Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
  • Centre de recherche en organogenèse expérimentale, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 2011-05-01 to 2020-09-22
  • Centre thématique de recherche en neurosciences, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 1999-06-01 to 2020-10-18
  • Étude de l'impact de l'innervation cutanée dans le Psoriasis, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2018-04-01 to 2023-03-31
  • Modélisation par génie tissulaire de la modulation de l'angiogenèse par l'innervation sensorielle, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2018-04-01 to 2023-03-31
  • Pathogenic Pathways and in-vitro modelization of Intracranial Aneurysms in populations of Inuit and French Canadian descent, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2017-04-01 to 2022-03-31
  • Reprogramming hiPSCs from ARSACS patients into neural cells in order to recreate a model of the disease in vitro, Subvention, National Ataxia Foundation, from 2019-03-01 to 2020-02-29
  • Stratégies de réparation des transsections des nerfs périphériques par génie tissulaire, Subvention, Instituts de recherche en santé du Canada, Volet Projet: Concours pilotes, from 2016-07-01 to 2021-06-30

Recently finished projects

  • Human inducible pluripotent stem cells (IPSC) plateform, Subvention, La Fondation Neuro Canada / Brain Canada, Soutien aux plateformes technologiques (SSPT), from 2015-04-01 to 2018-09-30
  • Implication des kinines dans la DMLA neovasculaire, Subvention, Fonds de recherche du Québec - Santé, Recherches sur la dégénérescence maculaire liée à l'âge (Programme DMLA), from 2017-04-01 to 2019-03-31
  • Phenotyping ARSACS in vitro using hiPSCs reprogrammed from patients and differentiated into motor neurons and Schwann cells, Subvention, Fondation de l'ataxie Charlevoix-Saguenay, from 2018-06-01 to 2019-05-31
Data provided by the Université Laval research projects registery