Dr. François Berthod is a professor at the Department of Surgery of the Laval University School of Medicine, and researcher at LOEX, Hôpital de l’Enfant-Jésus, CHU de Québec-Laval University research center.

The main objective of his research program is to use tissue-engineering techniques of in vitro organ reconstruction to model different diseases that can be induced, or modulated by the nervous system. These models will be useful to better understand the disease mechanisms and to find new therapeutic approaches. In addition, one of his projects aims to repair peripheral nerve injuries through the development of a nerve tube produced by tissue engineering with the patient’s own cells.

Development of in vitro models of diseases by tissue engineering

Many human diseases have causes and mechanisms that are still poorly understood, making it difficult to find more efficient drugs. Using patient cells, it is possible to reconstruct organs affected by these diseases, and recapitulate their effects in vitro to better understand their mechanisms, and use these models to test the efficacy of new drugs.

In the case of modeling psoriasis, Dr. Berthod’s team hypothesized that cutaneous innervation could play a major role in lesion formation, and developed, in collaboration with Dr. Roxane Pouliot, an innervated and immunocompetent psoriatic skin model to study this disease.

To model amyotrophic lateral sclerosis, a neurodegenerative disease, Dr. Berthod’s team uses tissue engineering tools. Motor neurons are cultured with astrocytes, microglia, Schwann cells, and myotubes in a compartmentalized three-dimensional construct to recapitulate the neurodegenerative process.

Repair of peripheral nerves by tissue engineering

Peripheral nerve injuries cause paralysis of the affected limbs. The current surgical options for repairing major deficits are ineffective. Dr. Berthod has developed, in collaboration with Dr. Hélène Khuong, neurosurgeon, a project to produce a nerve tube made up of the patient’s own cells, and tailored to bridge the nerve gap. The production of an autologous living tube is a major innovation that should greatly stimulate axonal migration in the graft and promote regeneration of the injured nerve, allowing motor and sensitive recoveries of the limb.

1401, 18e Rue
Local: R-205 - LOEX / CMDGT
Québec, Québec
Canada G1J 1Z4
40 entries « 2 of 4 »

Blais M, Parenteau-Bareil R, Cadau S, Berthod F

Concise review: tissue-engineered skin and nerve regeneration in burn treatment.

Journal Article

Stem Cells Transl Med, 2 (7), pp. 545-51, 2013, ISSN: 2157-6564.

Abstract | Links:

Cadau S, Rosignoli C, Rhetore S, Voegel J, Parenteau-Bareil R, Berthod F

Early stages of hair follicle development: a step by step microarray identity.

Journal Article

Eur J Dermatol, 2013, ISSN: 1167-1122.

Abstract | Links:

Berthod F

Fibroblasts and endothelial cells : the basic angiogenic unit (Chapter 7)

Book Chapter

G, Santulli (Ed.): Angiogenesis: insights from a systematic overview, pp. 145-157, New York, Nova Science Publishers, 2013, ISBN: 978-1-62618-114-4.

| Links:

Berthod F, Symes J, Tremblay N, Medin JA, Auger FA

Spontaneous fibroblast-derived pericyte recruitment in a human tissue-engineered angiogenesis model in vitro.

Journal Article

J Cell Physiol, 227 (5), pp. 2130-7, 2012, ISSN: 0021-9541.

Abstract | Links:

Berthod F, Gros-Louis F

In vivo and in vitro models to study amyotrophic lateral sclerosis (Chapter 4)

Book Chapter

M, Maurer (Ed.): Amyotrophic Lateral Sclerosis, pp. 81-124, InTech, 2012, ISBN: 978-953-307-806-9.

| Links:

Parenteau-Bareil R, Gauvin R, Cliche S, Gariepy C, Germain L, Berthod F

Comparative study of bovine, porcine and avian collagens for the production of a tissue engineered dermis.

Journal Article

Acta Biomater, 7 (10), pp. 3757-65, 2011, ISSN: 1742-7061.

Abstract | Links:

Gagnon V, Larouche D, Parenteau-Bareil R, Gingras M, Germain L, Berthod F

Hair follicles guide nerve migration in vitro and in vivo in tissue-engineered skin.

Journal Article

J Invest Dermatol, 131 (6), pp. 1375-8, 2011, ISSN: 0022-202X.

| Links:

Auclair-Daigle C, Berthod F

Potential of Tissue Engineering and Neural Stem Cells in the Understanding and Treatment of Neurodegenerative Diseases

Book Chapter

L, Song (Ed.): Stem Cells and Tissue Engineering, pp. 183-203, New Jersey, World Scientific Publishing Co., 2011.

| Links:

Rochon MH, Fradette J, Fortin V, Tomasetig F, Roberge CJ, Baker K, Berthod F, Auger FA, Germain L

Normal human epithelial cells regulate the size and morphology of tissue-engineered capillaries.

Journal Article

Tissue Eng Part A, 16 (5), pp. 1457-68, 2010, ISSN: 1937-3341.

Abstract | Links:

Berthod F, Germain L, Pouliot R, Auger FA

How to achieve early vascularization of tissue-engineered skin substitutes.

Book Chapter

ChandanK, Sen (Ed.): Advances in Wound Care, 1 , pp. 445-450, New Rochelle, NY, Mary Ann Liebert, Inc., 2010.

40 entries « 2 of 4 »
Signaler des ajouts ou des modifications

Active projects

  • Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
  • Centre de recherche en organogenèse expérimentale, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 2011-05-01 to 2020-09-22
  • Centre thématique de recherche en neurosciences, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 1999-06-01 to 2020-10-18
  • Étude de l'impact de l'innervation cutanée dans le Psoriasis, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2018-04-01 to 2023-03-31
  • Modélisation par génie tissulaire de la modulation de l'angiogenèse par l'innervation sensorielle, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2018-04-01 to 2023-03-31
  • Pathogenic Pathways and in-vitro modelization of Intracranial Aneurysms in populations of Inuit and French Canadian descent, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2017-04-01 to 2022-03-31
  • Reprogramming hiPSCs from ARSACS patients into neural cells in order to recreate a model of the disease in vitro, Subvention, National Ataxia Foundation, from 2019-03-01 to 2020-02-29
  • Stratégies de réparation des transsections des nerfs périphériques par génie tissulaire, Subvention, Instituts de recherche en santé du Canada, Volet Projet: Concours pilotes, from 2016-07-01 to 2021-06-30

Recently finished projects

  • Human inducible pluripotent stem cells (IPSC) plateform, Subvention, La Fondation Neuro Canada / Brain Canada, Soutien aux plateformes technologiques (SSPT), from 2015-04-01 to 2018-09-30
  • Implication des kinines dans la DMLA neovasculaire, Subvention, Fonds de recherche du Québec - Santé, Recherches sur la dégénérescence maculaire liée à l'âge (Programme DMLA), from 2017-04-01 to 2019-03-31
  • Phenotyping ARSACS in vitro using hiPSCs reprogrammed from patients and differentiated into motor neurons and Schwann cells, Subvention, Fondation de l'ataxie Charlevoix-Saguenay, from 2018-06-01 to 2019-05-31
Data provided by the Université Laval research projects registery