Christian Salesse is a full-time researcher at the Centre de recherche du CHU de Québec – Laval University, and full professor in Laval University’s Department of Ophthalmology. He graduated in biophysics from Université du Québec à Trois-Rivières (UQTR). He was then a postdoctoral fellow at the Johannes-Gutenberg Universität in Mainz, Germany. He then became professor at the UQTR for 14 years until he moved on to work at Laval University, in 2002. He has been a fellow from Fonds de Recherche – Québec Santé (FRQS) from the Junior 1 to the National level. He has also been Adjunct Professor at Jilin University in Changchun, China since 1995. He was an invited professor at several universities in France and Portugal.

His expertise is in the field of visual phototransduction and visual cycle, and the proteins involved in these processes. These processes require the binding of most of these proteins to membranes. We therefore perform membrane binding measurements of proteins. We are using simple model membranes such as lipid monolayers and bilayers to characterize their membrane binding. Protein binding to monolayers of saturated and highly polyunsaturated neutral or negatively charged lipids, that are typical of photoreceptors, is characterized by performing measurements of maximum insertion pressure, fluorescence microscopy, ellipsometry, as well as infrared spectroscopy (PM-IRRAS which also allows to determine protein orientation). Steady state fluorescence spectroscopy and fluorescence lifetime are used to characterize protein binding to lipid bilayers. The proteins we are studying also often have enzymatic activities; we have thus developed methods to characterize the activity of some of these enzymes. Moreover, we use nuclear magnetic resonance to determine the structure of a protein involved in the visual cycle.

Hôpital Saint-Sacrement
1050, chemin Sainte-Foy
CUO-Recherche
K2-02
Québec, Québec
Canada G1S 4L8
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Valeria Tsukanova, David W. Grainger, Salesse C

Monolayer behavior of NBD-labeled phosphatidylethanolamine and phosphatidylcholine at the air/water interface

Journal Article

Langmuir, 18 , pp. 5539-5550, 2002, ISSN: 0743-7463.

Desmeules P, Grandbois M, Bondarenko VA, Yamazaki A, Salesse C

Measurement of membrane binding between recoverin, a calcium-myristoyl switch protein, and lipid bilayers by AFM-based force spectroscopy.

Journal Article

Biophys J, 82 (6), pp. 3343-50, 2002, ISSN: 0006-3495.

Abstract | Links:

Valeria Tsukanova, Hugo Lavoie, A. Harata, T. Ogawa, Salesse C

Microscopic organization of long-chain rhodamine molecules in monolayers at the air/water interface

Journal Article

J Phys Chem, 106 , pp. 4303-4313, 2002, ISSN: 0022-3654.

Mons S, Salesse C, Mioskowski C, Lebeau L

Highly cohesive monolayers of lipid derivatives of colchicine: a dynamics study.

Journal Article

Chem Phys Lipids, 114 (1), pp. 99-102, 2002, ISSN: 0009-3084.

Abstract | Links:

Trudel E, Gallant J, Mons S, Mioskowski C, Lebeau L, Jeuris K, Foubert P, De Schryver F, Salesse C

Design of functionalized lipids and evidence for their binding to photosystem II core complex by oxygen evolution measurements, atomic force microscopy, and scanning near-field optical microscopy.

Journal Article

Biophys J, 81 (1), pp. 563-71, 2001, ISSN: 0006-3495.

Abstract | Links:

Vincent SP, Grenier S, Mioskowski C, Salesse C, Lebeau L

Probing the transducin nucleotide binding site with GDP analogues.

Journal Article

Bioorg Med Chem Lett, 11 (9), pp. 1185-8, 2001, ISSN: 0960-894X.

Abstract | Links:

Van Themsche C, Jacob M, Salesse C

Human retinal pigment epithelium secretes a phospholipase A2 and contains two novel intracellular phospholipases A2.

Journal Article

Biochem Cell Biol, 79 (1), pp. 1-10, 2001, ISSN: 0829-8211.

Abstract | Links:

Larouche K, Leclerc S, Salesse C, Guérin SL

Expression of the alpha 5 integrin subunit gene promoter is positively regulated by the extracellular matrix component fibronectin through the transcription factor Sp1 in corneal epithelial cells in vitro.

Journal Article

J Biol Chem, 275 (50), pp. 39182-92, 2000, ISSN: 0021-9258.

Abstract | Links:

Grandbois M, Desbat B, Salesse C

Monitoring of phospholipid monolayer hydrolysis by phospholipase A2 by use of polarization-modulated Fourier transform infrared spectroscopy.

Journal Article

Biophys Chem, 88 (1-3), pp. 127-35, 2000, ISSN: 0301-4622.

Abstract | Links:

Mons S, Veretout F, Carlier M, Erk I, Lepault J, Trudel E, Salesse C, Ducray P, Mioskowski C, Lebeau L

The interaction between lipid derivatives of colchicine and tubulin: consequences of the interaction of the alkaloid with lipid membranes.

Journal Article

Biochim Biophys Acta, 1468 (1-2), pp. 381-95, 2000, ISSN: 0006-3002.

Abstract | Links:

78 entries « 7 of 8 »
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Active projects

  • Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
  • Déterminants moléculaires de la lécithine rétinol acyltransférase responsables de la dégénérescence des photorécepteurs, Subvention, Instituts de recherche en santé du Canada, Volet Projet: Concours pilotes, from 2016-07-01 to 2021-06-30
  • Implication de diverses protéines dans la fonction des photorécepteurs visuels, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2017-04-01 to 2022-03-31
  • PROTEO, le regroupement québécois de recherche sur la fonction, l'ingénierie et les applications des protéines (PROTEO), Subvention, Fonds de recherche du Québec - Nature et technologies, Regroupements stratégiques NT, from 2015-04-01 to 2022-03-31
  • Regroupement québécois de recherche sur la fonction, la structure et l'ingénierie des protéines, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 2014-05-01 to 2023-04-03

Recently finished projects

  • Journée annuelle de la recherche en ophtalmologie , Subvention, Bausch Health, Canada Inc., from 2019-04-23 to 2020-03-31
  • Système FPLC pour la purification des protéines, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions d'outils et d'instruments de recherche (OIR), from 2019-03-28 to 2020-03-31
Data provided by the Université Laval research projects registery