Christian Salesse is a full-time researcher at the Centre de recherche du CHU de Québec – Laval University, and full professor in Laval University’s Department of Ophthalmology. He graduated in biophysics from Université du Québec à Trois-Rivières (UQTR). He was then a postdoctoral fellow at the Johannes-Gutenberg Universität in Mainz, Germany. He then became professor at the UQTR for 14 years until he moved on to work at Laval University, in 2002. He has been a fellow from Fonds de Recherche – Québec Santé (FRQS) from the Junior 1 to the National level. He has also been Adjunct Professor at Jilin University in Changchun, China since 1995. He was an invited professor at several universities in France and Portugal.

His expertise is in the field of visual phototransduction and visual cycle, and the proteins involved in these processes. These processes require the binding of most of these proteins to membranes. We therefore perform membrane binding measurements of proteins. We are using simple model membranes such as lipid monolayers and bilayers to characterize their membrane binding. Protein binding to monolayers of saturated and highly polyunsaturated neutral or negatively charged lipids, that are typical of photoreceptors, is characterized by performing measurements of maximum insertion pressure, fluorescence microscopy, ellipsometry, as well as infrared spectroscopy (PM-IRRAS which also allows to determine protein orientation). Steady state fluorescence spectroscopy and fluorescence lifetime are used to characterize protein binding to lipid bilayers. The proteins we are studying also often have enzymatic activities; we have thus developed methods to characterize the activity of some of these enzymes. Moreover, we use nuclear magnetic resonance to determine the structure of a protein involved in the visual cycle.

Hôpital Saint-Sacrement
1050, chemin Sainte-Foy
CUO-Recherche
Québec, Québec
Canada G1S 4L8
+1 418-682-7569, extension 87569
+1 418-682-8000
christian.salesse@crchude... christian.salesse@fmed.ul...

Latest news

78 entries « ‹ 1 of 8 › »

Gauthier ME, Roy S, Cantin L, Salesse C

Comparison between the enzymatic activity, structure and substrate binding of mouse and human lecithin retinol acyltransferase.

Journal Article

Biochem Biophys Res Commun, 519 (4), pp. 832-837, 2019, ISSN: 0006-291X.

Abstract | Links:

Bernier SC, Morency LP, Najmanovich R, Salesse C

Identification of an alternative translation initiation site in the sequence of the commonly used Glutathione S-Transferase tag.

Journal Article

J Biotechnol, 286 , pp. 14-16, 2018, ISSN: 0168-1656.

Abstract | Links:

Potvin-Fournier K, Valois-Paillard G, Gagnon MC, Lefevre T, Audet P, Cantin L, Paquin JF, Salesse C, Auger M

Novel approaches to probe the binding of recoverin to membranes.

Journal Article

Eur Biophys J, 47 (6), pp. 679-691, 2018, ISSN: 0175-7571.

Abstract | Links:

Cyr N, Smith TK, Boisselier E, Leroux LP, Kottarampatel AH, Davidsen A, Salesse C, Jardim A

The hydrophobic region of the Leishmania peroxin 14: requirements for association with a glycosome mimetic membrane.

Journal Article

Biochem J, 475 (2), pp. 511-529, 2018, ISSN: 0264-6021.

Abstract | Links:

Bernier SC, Cantin L, Salesse C

Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags.

Journal Article

Protein Expr Purif, 152 , pp. 92-106, 2018, ISSN: 1046-5928.

Abstract | Links:

Potvin-Fournier K, Valois-Paillard G, Lefevre T, Cantin L, Salesse C, Auger M

Membrane fluidity is a driving force for recoverin myristoyl immobilization in zwitterionic lipids.

Journal Article

Biochem Biophys Res Commun, 490 (4), pp. 1268-1273, 2017, ISSN: 0006-291X.

Abstract | Links:

Boisselier E, Demers E, Cantin L, Salesse C

How to gather useful and valuable information from protein binding measurements using Langmuir lipid monolayers.

Journal Article

Adv Colloid Interface Sci, 243 , pp. 60-76, 2017, ISSN: 0001-8686.

Abstract | Links:

Salesse C

[Physiology of the visual retinal signal: From phototransduction to the visual cycle].

Journal Article

J Fr Ophtalmol, 40 (3), pp. 239-250, 2017, ISSN: 0181-5512.

Abstract | Links:

Calvez P, Schmidt TF, Cantin L, Klinker K, Salesse C

Phosphatidylserine Allows Observation of the Calcium-Myristoyl Switch of Recoverin and Its Preferential Binding.

Journal Article

J Am Chem Soc, 138 (41), pp. 13533-13540, 2016, ISSN: 0002-7863.

Abstract | Links:

Potvin-Fournier K, Lefevre T, Picard-Lafond A, Marcotte C, Dufresne C, Cantin L, Salesse C, Auger M

Discriminating Lipid- from Protein-Calcium Binding To Understand the Interaction between Recoverin and Phosphatidylglycerol Model Membranes.

Journal Article

Biochemistry, 55 (24), pp. 3481-91, 2016, ISSN: 0006-2960.

Abstract | Links:

78 entries « ‹ 1 of 8 › »
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Active projects

  • Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
  • Déterminants moléculaires de la lécithine rétinol acyltransférase responsables de la dégénérescence des photorécepteurs, Subvention, Instituts de recherche en santé du Canada, Volet Projet: Concours pilotes, from 2016-07-01 to 2021-06-30
  • Implication de diverses protéines dans la fonction des photorécepteurs visuels, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2017-04-01 to 2022-03-31
  • PROTEO, le regroupement québécois de recherche sur la fonction, l'ingénierie et les applications des protéines (PROTEO), Subvention, Fonds de recherche du Québec - Nature et technologies, Regroupements stratégiques NT, from 2015-04-01 to 2021-03-31
  • Regroupement québécois de recherche sur la fonction, la structure et l'ingénierie des protéines, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 2014-05-01 to 2020-04-30

Recently finished projects

  • La recoverine (TagR) comme une nouvelle étiquette de fusion pour faciliter la solubilisation et la purification des protéines recombinantes, Subvention, Ministère de l'Économie, de la Science et de l'Innovation, Programme de soutien à la valorisation et au transfert PSVT - Soutien aux projets structurants (volets 2a, 2b, 2c), from 2018-01-10 to 2018-07-10
  • Système FPLC pour la purification des protéines, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions d'outils et d'instruments de recherche (OIR), from 2019-03-28 to 2020-03-31
Data provided by the Université Laval research projects registery