Dr. Amélie Fradet-Turcotte, Ph.D., owns a Canada Research Chair in molecular virology and genomic instability and is an assistant professor in the Department of molecular biology, medical biochemistry and pathology of the Laval University School of Medicine. Recruited to the CHU de Québec research center in September 2015, she is a member of the St-Patrick Research Group in Fundamental Oncology and of Laval University’s Cancer Research Center (CRC).     

The main interest of Dr. Fradet-Turcotte’s laboratory is to understand how the mechanisms that safeguard genomic integrity in our cells are challenged during viral infections. Her laboratory uses a combination of molecular biology, biochemistry, and cellular biology to determine how the infection by DNA viruses such as the human papillomavirus (HPV) impacts the genomic integrity of the infected cell and to tackle how viruses usurp the DNA-damage machinery to promote the viral life cycle. Specifically, the work in her laboratory aims at elucidating the following questions: 1) What is the interplay between HPV and the DSB signaling and repair proteins, and how does it change during carcinogenesis? 2) How does HPV impact the functions of the reader of ubiquitylated chromatin in cancer cells? 3) What are the consequences of viral infection on DSB signaling and DNA repair pathway choice, and how does this affect the resistance of HPV+ cancer cells to current chemo- and radiotherapies?

Every day, many different types of DNA damage threaten the integrity of our genome. A failure to properly repair these alterations can result in the acquisition of hallmarks of cancer such as translocations and somatic mutations, or can lead to cell death. By using HPV as a model, the outcomes of these studies will not only improve our understanding of the mechanisms that safeguard genomic stability in our cells, but they will also have important implications for HPV-dependent cancer biology (cervical and oropharyngeal cancers), as well as for our understanding of the viral life cycle.

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32 entries « 2 of 4 »

Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J

High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.

Journal Article

Cell, 163 (6), pp. 1515-26, 2015, ISSN: 0092-8674.

Abstract | Links:

Gagnon D, Fradet-Turcotte A, Archambault J

A quantitative and high-throughput assay of human papillomavirus DNA replication.

Journal Article

Methods Mol Biol, 1249 , pp. 305-16, 2015, ISSN: 1064-3745.

Abstract | Links:

Lehoux M, Fradet-Turcotte A, Archambault J

Methods to assess the nucleocytoplasmic shuttling of the HPV E1 helicase and its effects on cellular proliferation and induction of a DNA damage response.

Journal Article

Methods Mol Biol, 1249 , pp. 67-80, 2015, ISSN: 1064-3745.

Abstract | Links:

Orthwein A, Fradet-Turcotte A, Noordermeer SM, Canny MD, Brun CM, Strecker J, Escribano-Diaz C, Durocher D

Mitosis inhibits DNA double-strand break repair to guard against telomere fusions.

Journal Article

Science, 344 (6180), pp. 189-93, 2014, ISSN: 0036-8075.

Abstract | Links:

Bohgaki M, Bohgaki T, El Ghamrasni S, Srikumar T, Maire G, Panier S, Fradet-Turcotte A, Stewart GS, Raught B, Hakem A, Hakem R

RNF168 ubiquitylates 53BP1 and controls its response to DNA double-strand breaks.

Journal Article

Proc Natl Acad Sci U S A, 110 (52), pp. 20982-7, 2013, ISSN: 0027-8424.

Abstract | Links:

Fradet-Turcotte A, Canny MD, Escribano-Diaz C, Orthwein A, Leung CC, Huang H, Landry MC, Kitevski-LeBlanc J, Noordermeer SM, Sicheri F, Durocher D

53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark.

Journal Article

Nature, 499 (7456), pp. 50-4, 2013, ISSN: 0028-0836.

Abstract | Links:

Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D

A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and BRCA1-CtIP controls DNA repair pathway choice.

Journal Article

Mol Cell, 49 (5), pp. 872-83, 2013, ISSN: 1097-2765.

Abstract | Links:

Panier S, Ichijima Y, Fradet-Turcotte A, Leung CC, Kaustov L, Arrowsmith CH, Durocher D

Tandem protein interaction modules organize the ubiquitin-dependent response to DNA double-strand breaks.

Journal Article

Mol Cell, 47 (3), pp. 383-95, 2012, ISSN: 1097-2765.

Abstract | Links:

Lehoux M, Fradet-Turcotte A, Lussier-Price M, Omichinski JG, Archambault J

Inhibition of human papillomavirus DNA replication by an E1-derived p80/UAF1-binding peptide.

Journal Article

J Virol, 86 (7), pp. 3486-500, 2012, ISSN: 0022-538X.

Abstract | Links:

Fradet-Turcotte A, Bergeron-Labrecque F, Moody CA, Lehoux M, Laimins LA, Archambault J

Nuclear accumulation of the papillomavirus E1 helicase blocks S-phase progression and triggers an ATM-dependent DNA damage response.

Journal Article

J Virol, 85 (17), pp. 8996-9012, 2011, ISSN: 0022-538X.

Abstract | Links:

32 entries « 2 of 4 »
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Active projects

  • Centre de recherche du CHU de Québec - Université Laval, Subvention, Centre hospitalier universitaire de Québec - Université Laval, Centres de recherche affiliés, from 2017-01-01 to 2099-12-31
  • Centre de recherche sur le cancer, Subvention, Institutionnel - BDR, BDR - Centres de recherche reconnus, from 1996-05-01 to 2022-06-13
  • Characterisation of PTM-associated mutations that alter signaling network in breast and ovarian cancers, Subvention, Société de recherche sur le cancer, Subvention de fonctionnement, from 2019-09-01 to 2021-08-31
  • Deciphering the impact of chromatin modifications on DNA repair processes., Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions à la découverte SD (individuelles et d'équipe), from 2016-04-01 to 2021-03-31
  • Defining the molecular basis of chemoradiation sensitivity in HPV+ head and neck cancers, Subvention, Instituts de recherche en santé du Canada, Subvention Projet, from 2017-04-01 to 2022-03-31
  • Engineering HPV oncoprotein E6 for inhibitors of cancer progression, Subvention, Société de recherche sur le cancer, from 2019-08-01 to 2021-07-31
  • Fonds Jeanne-et-Jean-Louis-Lévesque en soutien à la Chaire de recherche en virologie moléculaire et instabilité génomique, Subvention, Fondation de l'Université Laval, from 2017-03-01 to 2022-02-28

Recently finished projects

  • Discovery and validation of ubiquitin-associated mutations in the DNA-damage response pathway in breast cancer, Subvention, Société de recherche sur le cancer, Subvention de fonctionnement, from 2016-04-01 to 2019-08-31
  • Efficient and reliable shearing of chromatin for highthroughput analysis, Subvention, Conseil de recherches en sciences naturelles et génie Canada, Subventions d'outils et d'instruments de recherche (OIR), from 2019-03-28 to 2020-03-31
  • Programme de soutien aux nouveaux chercheurs de la Faculté de médecine, Subvention, Université Laval - Soutien à la recherche, Soutien facultaire aux professeurs chevronnés de la Faculté de médecine, from 2017-05-01 to 2019-04-30
Data provided by the Université Laval research projects registery